中国农业科学 ›› 2006, Vol. 39 ›› Issue (12): 2603-2607 .doi: 10.3864/j.issn.0578-1752.at-2005-5710

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

水溶性灵芝肽在动物体外的抗氧化活性

何 慧,孙 颉,谢笔钧   

  1. 华中农业大学食品科技学院
  • 收稿日期:2005-07-14 修回日期:1900-01-01 出版日期:2006-12-10 发布日期:2006-12-10

The Antioxidative Activity of Ganoderma lucidum Peptides in Vitro Test

,,   

  1. 华中农业大学食品科技学院
  • Received:2005-07-14 Revised:1900-01-01 Published:2006-12-10 Online:2006-12-10

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19

摘要： 【目的】研究水溶性灵芝肽在动物体外的抗氧化活性。【方法】用丙二醛(MDA)试剂盒测定MDA含量，用分光光度法测定大鼠血清自氧化溶血及小鼠肝线粒体肿胀度。【结果】当水溶性灵芝肽的剂量为0.25 mg•ml-1时，对大鼠红细胞自氧化溶血的抑制率达62.33%，对溶血过程中MDA生成的抑制率达91.24%；在有或无自由基诱导剂（Fe2+或H2O2）时，0.35 mg•ml-1的水溶性灵芝肽均能极显著抑制小鼠肝匀浆中MDA的生成（P＜0.01），且抑制率大致相当，为60%左右；当水溶性灵芝肽剂量为1.00 mg•ml-1时，对线粒体肿胀度的抑制率为90.52%，对线粒体中MDA生成的抑制率达65.42%，且呈明显剂量-效应关系。【结论】水溶性灵芝肽在体外具有明显的抗氧化作用。

关键词: 灵芝, 肽, 抗氧化, 体外试验

Abstract: Abstract: The antioxidative activity of Ganoderma lucidum peptides (GLP) in vitro test was studied. The reagent kits were used to determine the content of malondialdehyde (MDA). The auto-hemolysis of mice red blood cells (RBC) and the swelling of mice liver mitochondria were observed by means of spectrophotometer. The results showed that the inhibiting rate of 0.25 mg·ml-1 GLP on the auto-hemolysis of mice RBC was 62.3% and the inhibiting rate on the MDA formation in mice RBC was 91.2%. 0.35 mg ml-1 GLP could extremely significantly prevent the MDA formation in mice liver homogenate ( p<0.01) with or without free radicals inducers (Fe2+or H2O2). They had the near inhibiting rate-around 60%. When 1.00 mg·ml-1 GLP was added, the inhibiting rate on the swelling of mice liver mitochondria was 90.5% and the inhibiting rate on the MDA formation in mice liver mitochondria was 65.4% in a dose-dependent manner. Conclusion: GLP was a good antioxidant in vitro test.

Key words: Ganoderma lucidum, peptides, antioxidation

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