中国农业科学 ›› 2006, Vol. 39 ›› Issue (12): 2597-2602 .

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

动物、环境及饲养员多重耐药大肠杆菌的PFGE分型研究

杨玲丽,陈杖榴,刘健华,曾振灵,马竣英,吴聪明   

  1. 华南农业大学兽医学院
  • 收稿日期:2005-09-12 修回日期:1900-01-01 出版日期:2006-12-10 发布日期:2006-12-10
  • 通讯作者: 刘健华 liujianhua

Genotyping of Multidrug-resistant E.coli Isolated from Animals, Farmers and Environment by Pulsed-field Gel Electrophoresis

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  1. 华南农业大学兽医学院
  • Received:2005-09-12 Revised:1900-01-01 Online:2006-12-10 Published:2006-12-10

摘要: 【目的】了解养殖场多重耐药大肠杆菌耐药性的传播机制,探讨人、动物与环境间耐药菌垂直克隆传播的可能性。【方法】采用脉冲场凝胶电泳(PFGE)分型方法对分离自养殖场动物、环境和饲养员的86株耐药谱相近多重耐药大肠杆菌进行DNA指纹图谱分型,比较菌株之间的亲缘关系。【结果】37株鸡场来源大肠杆菌可分为20种PFGE分型;49株猪场来源大肠杆菌可分为24种PFGE分型;同一动物、环境或饲养员采样点分离菌株PFGE指纹图谱相同者较多;同一养殖场不同动物个体、养殖场环境不同采样点、动物和环境以及饲养员和环境都存在PFGE指纹图谱完全相同菌株;鸡场和猪场都发现有属同一PFGE分型的来自动物、饲养员和环境的菌株;86株菌全部对环丙沙星、氨苄西林、链霉素、四环素、多西环素和复方新诺明耐药,相同PFGE分型的菌株其耐药谱型不一定相同。【结论】养殖场动物、环境及饲养员之间存在多重耐药大肠杆菌的克隆传播。

关键词: 大肠杆菌, 多重耐药, PFGE分型, 动物, 克隆传播

Abstract: To study the resistance transfer mechanism of E.coli in animal farms and the possibility of resistant clones vertical spread between animals and huamns, 86 multidrug-resistant E.coli isolates with similar resistance profiles were characterized by pulsed-field gel electrophoresis(PFGE) after XbaI digestion and drug susceptibility testing to investigate the epidemiologic relationship among the isolates from animals, environment and farmers. The results showed that twenty different PFGE types were found among 37 strains isolated from poultry farm and twenty-four were found among 49 strains isolated from swine farm. The PFGE patterns of strains isolated from the same animal or farmer or environment were often identical. Some E. coli isolates from different animals in the same farm have identical PFGE patterns and some isolates from animals or farmers were identical to some isolates from environments. The PFGE patterns of five E.coli strains, one from chicken, three from one farmer and one from environment were quite heterogeneous. Three E.coli isolates from pig, farmer and environment separately were also found belong to the same PFGE genetype. All isolates were resistant to ciprofloxacin, ampicillin, streptomycin, tetracycline, doxycycline and trimethoprim-sulfamethoxazole. Resistance patterns of isolates with identical PFGE genetype may different. And the same resistance profile may shared by isolates with different PFGE genetype. It is concluded that vertical transmission of resistant E. coli clones between farm animals, environment and farmers were commonly occurs. Resistance spectra and PFGE genetypes were not always uniform.

Key words: E.coli, Multidrug-resistant, PFGE genetype, Animals, Clonal spread