关键词: 芸芥, RAPD技术, 遗传多样性

Abstract: 【Objective】The studies of genetic diversity of Yunjie (E. Mill.) accessions and relationships among them are essential for the utilization of them. 【Method】Polymorphism of entire genome of thirty cultivars from Europe, Africa and Asia representing different Yunjie-growing ecotypes was assessed by analyses of RAPD makers. 【Result】Yunjie have abundant polymorphism. Forty-seven out of seventy-four tested random decamer primers were able to identify 486 stable RAPD bands from these Yunjie cultivas. Of them 432 bands, or 89.0% of the total were polymophic. Every group of Yunjie accessions had their characteristic DNA bands. Cluster analysis by unweighted pair group method of arithmetic means（UPGMA）suggested that the 30 Yunjie genotypes could be divided into seven groups, Chinese Yunjie become a group independently and Spain Yunjie belongs to other six different groups. 【Conclusion】The study shows that Spain is richer in Yunjie germplasm diversity than other regions, which is in good agreement with the opinion that west Mediterranean is the origin center and diversity center of Yunjie. The result suggests that China is the second evaluation center of Yunjie.

Key words: Yunjie (Eruca Mill.), RAPD, Genetic diversity