中国农业科学 ›› 2006, Vol. 39 ›› Issue (02): 295-299 .

• 植物保护 • 上一篇    下一篇

利用分子标记解析小麦新种质YW243的抗锈病基因

刘燕,张增艳,辛志勇,林志珊,杜丽璞,徐惠君,刘 丽,陈孝,张改生   

  • 收稿日期:2005-08-20 修回日期:2005-12-04 出版日期:2006-02-10 发布日期:2006-02-10

Analysis on the Rust Resistance Genes of a New Wheat Germplasm YW243 by Molecular Markers

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  • Received:2005-08-20 Revised:2005-12-04 Online:2006-02-10 Published:2006-02-10

摘要: 【目的】对兼抗条锈病、秆锈病、叶锈病、黄矮病、白粉病等5种病害的小麦新种质YW243中抗锈病基因的组成和来源进行解析。【方法】通过系谱推断,利用了抗条锈病基因Yr9、YrX、Yr2、Yr1以及抗秆锈病基因Sr31的特异PCR标记和醇溶蛋白蛋白质电泳图谱,分析YW243及其部分相关亲本材料丰抗8号、丰抗13、陕7859中相关的抗条锈病基因和抗秆锈病基因的有无,解析YW243中抗锈病基因的组成。【结果】YW243含有抗条锈病基因Yr1、Yr2、Yr9、YrX,抗秆锈病基因Sr31、抗叶锈病基因Lr26。Yr1源于亲本陕7859或丰抗13,Yr2源于陕7859或丰抗8号或丰抗13;YrX源于丰抗13;Yr9 、Sr31和Lr26源于陕7859或丰抗8号中。【结论】解析了YW243中抗条锈病基因和抗秆锈病基因的组成,证明YW243是一个含有Yr1、Yr2、Yr9、YrX、Sr31等抗锈病基因的多基因聚合体,这些基因特异的SSR,STS特异标记可用于检测多基因聚合体YW243中目标基因,为在抗锈育种中利用、选择YW243的抗锈基因提供了快捷方法。

关键词: 小麦, YW243, 分子标记, 抗锈病基因

Abstract: 【Objective】 A new wheat line YW243 is a recently developed new germplasm with multi-resistance to wheat stripe rust, stem rust, leaf rust, wheat yellow dwarf and powdery mildew. In this study, the gene composition of the stripe and stem rust resistance of YW243 was analyzed. In order to clarify the sources of the rust resistance genes of YW243, YW243 and its parents, such as Fengkang 8, Fengkang 13, Shan 7859, Suwon 11 and Heine Hvede, were analyzed by the molecular markers of the resistance genes . 【Method】 The composition and the sources of the stripe and stem rust resistance genes of YW243 were analyzed by using PCR markers of Yr9, YrX, Yr2, Yr1, Sr31 and SDS-PAGE. 【Result】 YW243 possessed at least four stripe rust resistance genes of Yr1, Yr2, Yr9 and YrX, one stem rust resistance gene Sr31. Shan 7859 and Fengkang13 are the sources of Yr1 in YW243, Shan7859, Fengkang 8 and Fengkang13 are the sources of Yr2 in YW243, Fengkang 13 is the source of YrX of YW243. Fengkang 8 and Shan 7859 are the sources of Yr9 and Sr31 of YW243. 【Conclusion】YW243 is a good resistance germplasm for wheat breeding in the future.

Key words: Wheat (Triticum aestivum Linn.), YW243, Molecular marker, Rust resistance gene