中国农业科学 ›› 2005, Vol. 38 ›› Issue (12): 2566-2570 .

• 研究简报 • 上一篇    下一篇

决明属牧草60Co-γ射线辐射后代的若干特性研究

翁伯琦,徐国忠,郑向丽,应朝阳,黄毅斌   

  • 收稿日期:2005-05-30 修回日期:1900-01-01 出版日期:2005-12-10 发布日期:2005-12-10

Study on Several Characters of Chamaecrista spp. Irradiated by 60Co -γ

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  • Received:2005-05-30 Revised:1900-01-01 Online:2005-12-10 Published:2005-12-10

摘要: 利用6利用60Co-γ射线辐射豆科决明属5个牧草品种的种子,通过M1~M3代田间试验观察和筛选,选取5个变异株系后代进行M4代盆栽试验。结果表明,其农艺性状有不同程度的变异,其中2219、86134、92985辐射后代生物量分别比原种增加10.4%、42.8%、20.74%,植株的氨基酸营养成分也呈现较大程度的变化。RAPD分析表明,辐射后代与原种的DNA指纹图谱存在明显差异。

关键词: 辐射, 决明属, 牧草, RAPD分析

Abstract: The seeds of five Chamaecrista spp. pasturages were irradiated by 60Co-γ. Based on the observations of M1, M2, M3 generation in fiel experiment, five plants with bigger variations in agronomic character were elected for pot experiment M4. The results showed that there were some differences in agronomic characters among the five cultivars and their parents without irradiation. The biomass of 2219, 86134 and 92985 after irradiation increased by 10.4%, 42.8% and 20.74%, respectively. The nutrient elements of plants also changed greatly. On the basis of analysis by using random amplified polymorphic DNA (RAPD) markers, it was found that there were obvious differences in DNA fingerprinting between the progenies after irradiation by 60Co-γ and their parents.

Key words: Irradiation, Chammaecrista, Pasturage, RAPD analysis