关键词: 山羊, 兔, 异种克隆, 连续核移植

Abstract: The experiments of serial nuclear transfer were conducted by using the blastomere of inter-species original or serial cloned morula as donor and rabbit oocyte as recipient. Finally serial I and serial II cloned embryos were obtained. Of these, Ⅰserial reconstructed embryos 58 andⅡserial reconstructed embryos 14 .The rate of fusion was 79.5% and 70% respectively and there was no significant difference between them(P>0.05). However, the rate of cleavage respectively was 75.9% and 28.6% and difference between them was very significant (P<0.05). Eventually there was noⅡserial blastocyst obtained, but the rate of Ⅰserial blastocyst was 10.2%.

Key words: Goat, Rabbit, Inter-species clone, Serial nuclear transfer