关键词: 小麦, EST-SSR, 遗传多样性

Abstract: EST-SSR marker is a new kind of SSR markers which is developed from the ESTs (expressed sequence tags). In this study, a total of 35 EST-SSR markers were used to analyze the genetic diversity and phylogeny of 96 wheat germplasms. All the primer sets produced clear and strong expected amplifications. 129 alleles were generated by the 35 loci, but most of the primer pairs produced 2 to 4 alleles. 87.6% of the 129 alleles showed polymorphic among the 96 wheat varieties. Primer pairs could be used to identify some varieties according to the specific PCR amplifications, to reveal the phylogeny between hexaploid wheat collections and their ancestral chromosome donors. Based on 0.745 similarity coefficient, synthesized double diploid varieties and hexaploid wheat were classified into different subgroups. Compared with SSR markers derived from genomic DNAs, EST-SSRs were parts of expressed genes. Therefore, when used in wheat genetic analysis, EST-SSR markers not only act as genetic markers, but also reveal differences in related gene expression.

Key words: Wheat, EST-SSR, Genetic diversity