中国农业科学 ›› 2004, Vol. 37 ›› Issue (11): 1699-1704 .

• 研究简报 • 上一篇    下一篇

龙须草生态型的划分及其遗传差异的AFLP分析

姚家玲,洪柳,张友德   

  1. 华中农业大学 生命科学技术学院
  • 收稿日期:2003-02-28 修回日期:2003-10-29 出版日期:2004-11-20 发布日期:2004-11-20
  • 通讯作者: 姚家玲

Genetic Diversity and Classification of Ecotypes of Eulaliopsis binata via Morphological Traits and AFLP Markers

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  1. 华中农业大学 生命科学技术学院
  • Received:2003-02-28 Revised:2003-10-29 Online:2004-11-20 Published:2004-11-20

摘要: 利用AFLP分子标记技术,结合形态学特征观察和叶片纤维素等成分含量的测定,将来自4个省的9个不同居群的龙须草划分为4种生态型:窄叶类型(湖北)、浓绿叶类型(广西)、红秆类型(陕西)和宽叶类型(河南);来自同一地区的不同居群之间,遗传差异很小,聚为同一种生态型;同一居群内不同无融合生殖个体之间未检测到差异;分析了不同生态型之间的遗传差异和亲缘关系;讨论了AFLP技术在无融合生殖植物的分类和遗传学研究中的应用前景。

关键词: 龙须草, 生态型, AFLP, 无融合生殖

Abstract: AFLP markers and morphological observation were used to identify the ecotypes of Eulaliopsis binata, which was reported as an apomictic species in Poaceae. The result showed that there is a large diversity among different ecotypes but a little within each population. By cluster analysis based on AFLP data, four ecotypes were identified as follows: slender-leaf ecotype (Hubei), deep-green-leaf ecotype(Guangxi), red-haulm ecotype (Shaanxi) and wide-leaf ecotype (Henan). The utilizations of AFLP markers in classification and further investigation of apomictic plants were discussed as well.

Key words: Eulaliopsis binat, Ecotype, AFLP, Apomixis