基于AFLP分子标记的核桃核心种质的构建

doi:10.3864/j.issn.0578-1752.2013.23.015

摘要/Abstract

摘要： 【目的】构建核桃核心种质以便更好地保存、评价和利用丰富的核桃种质资源。【方法】利用AFLP分子标记，对131份核桃原始种质采用逐步聚类法建立候选核心种质，比较不同候选核心种质的多态性位点数、多态性位点百分率，结合形态学指标及地理来源，最终确定核桃核心种质，并进行评价。【结果】建立的核桃核心种质保留了原始种质10%的样品，分别为河北的天桥1号、陕西的西洛2号和西林1号、山西的晋龙1号、山东的丰辉、辽宁的辽宁8号和辽73013、新疆的温185、河南的绿波、北京的北京746、美国引进品种维纳、日本引进品种清香和朝鲜的品种安边1号。根据遗传多样性评价结果，与原始种质相比，核心种质的多态性位点保留率为75.4%。【结论】构建的核桃核心种质能较大程度地代表原始种质的遗传信息，符合核心种质要求。

关键词: 核桃 , 核心种质 , AFLP , 遗传多样性

Abstract: 【Objective】 Walnut is an important economic tree species in China, and its germplasm resources are very rich. There were many problems in the processing of germplasm preservation such as large area occupied, higher management costs, which brought great difficulties to the conservation, utilization and evaluation. Therefore, construction of walnut core collection is very important for preservation, study and application of Juglans regia L. germplasm resources.【Method】 Based on AFLP markers, candidate core collections were constructed by using proportional strategy and UPGMA clustering sampling method within subgroups after dividing 131 Juglans regia germplasm into 45 subgroups. The core collection was eventually identified by comparison of the genetic diversity parameters of these candidate core collections, such as the number of polymorphic loci, percentage of polymorphic loci, and morphological indicators. 【Result】The core collections including Tianqiao1 from Hebei province, Xiluo2 and Xilin1 from Shaanxi province, Jinglong1 from Shanxi province, Fenghui from Shandong province, Liaoning8 and Liao73013 from Liaoning province, Wen185 from Xinjiang, Lvbo from Henan province, Beijing746 from Beijing, Vina from America, Qingxiang from Japan, Anbian1 from North Korea, reserved 10% samples of original collection. The retention rate of polymorphic loci was 75.4% in the core collection.【Conclusion】The core collection which was constructed in this study can largely represent the genetic information of the original germplasm, and it was matched with the requirements of the core collection.

Key words: Juglans regia L. , core collection , amplified fragment length polymorphism (AFLP) , genetic diversity

王红霞1, 赵书岗2, 高仪3, 玄立春4, 张志华1. 基于AFLP分子标记的核桃核心种质的构建[J]. 中国农业科学, 2013, 46(23): 4985-4995.

WANG Hong-Xia-1, ZHAO Shu-Gang-2, GAO Yi-3, XUAN Li-Chun-4, ZHANG Zhi-Hua-1. A Construction of the Core-Collection of Juglans regia L. Based on AFLP Molecular Markers[J]. Scientia Agricultura Sinica, 2013, 46(23): 4985-4995.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2013.23.015

https://www.chinaagrisci.com/CN/Y2013/V46/I23/4985

参考文献

[1]Frankle O H. Genetic perspectives of genmplasm conservation//Arber W, Illmensee K, Peacock W J, Starlinger P. Genetic Manipulation: Impact on Man and Society. Cambridge: Cambridge University Press, 1984: 161-170.

[2]Brown A H D. Core collection: A practical approach to genetic resources management. Genome, 1989, 31: 818-824.

[3]邱丽娟, 曹永生, 常汝镇, 周新安, 王国勋, 孙建英, 谢华, 张博, 李向华, 许占有, 刘立宏. 中国大豆(Glycine max)核心种质构建. 中国农业科学, 2003, 36(12): 1442-1449.

Qiu L J, Cao Y S, Chang R Z, Zhou X A, Wang G X, Sun J Y, Xie H, Zhang B, Li X H, Xu Z Y, Liu L H. Establishment of Chinese soybean (G.max) core collectionⅠ. sampling strategy. Scientia Agricultura Sinica, 2003, 36(12): 1442-1449. (in Chinese)

[4]Spagnoletti Zeuli P L, Qualset C O. Evaluation of five strategies for obtaining a core subset from a large genetic resource collection of durum wheat. Theoretical and Applied Genetics, 1993, 87: 295-304.

[5]Diwan N, Mcintosh M S, Bauchan G R. Methods of developing a core collection of annual Medicago species. Theoretical and Applied Genetics, 1995, 90: 755-761.

[6]Zewdie Y, Tong N, Bosland P. Establishing a core collection of Capsicum using a cluster analysis with enlightened selection of accessions. Genetic Resources and Crop Evolution, 2004, 51: 147-151.

[7]魏兴华, 汤圣祥, 余汉勇, 江云珠. 浙江粳稻地方品种核心样品的构建方法. 作物学报, 2001, 27(3): 324-328.

Wei X H, Tang S X, Yu H Y, Jiang Y Z. Methods of developing a core collection for Zhejiang traditional Japonica rice germplasm. Acta Agronomica Sinica, 2001, 27(3): 324-328. (in Chinese)

[8]张秀荣, 郭庆元, 赵应忠, 冯祥运, 周明德, Hodgkin T. 中国芝麻资源核心收集品研究. 中国农业科学, 1998, 31(3): 49-55.

Zhang X R, Guo Q Y, Zhao Y Z, Feng X Y, Zhou M D, Hodgkin T. Establishment of sesame germplasm core collection in China. Scientia Agricultura Sinica, 1998, 31(3): 49-55. (in Chinese)

[9]Hokanson S C, Szewc-Mcfadden A K, Lamboy W F, McFerson J R. Microsatellite(SSR)markers reveal genetic identities, genetic diversity and relationships in Malus×domestica Borkh. core subset collection. Theoretical and Applied Genetics, 1998, 97: 671-683.

[10]刘遵春, 张春雨, 张艳敏, 张小燕, 吴传金, 王海波, 石俊, 陈学森. 利用数量性状构建新疆野苹果核心种质的方法. 中国农业科学, 2010, 43(2): 358-370.

Liu Z C, Zhang C Y, Zhang Y M, Zhang X Y, Wu C J, Wang H B, Shi J, Chen X S. Study on method of constructing core collection of Malus sieversii based on quantitative traits. Scientia Agricultura Sinica, 2010, 43(2): 358-370. (in Chinese)

[11]李银霞, 安丽君, 姜全, 赵剑波, 李天红. 桃（Prunus persica (L.)Batsch）品种核心种质的构建与评价. 中国农业大学学报, 2007, 12(5): 22-28.

Li Y X, An L J, Jiang Q, Zhao J B, Li T H. Establishment and evaluation of the core collection of peach (Prunus persica (L.) Batsch.)cultivars. Journal of China Agricultural University, 2007, 12(5): 22-28. (in Chinese)

[12]张靖国, 胡红菊, 田瑞, 陈启亮, 杨晓平. 中国砂梨初级核心种质的构建. 湖北农业科学, 2011, 50(8): 1590-1592.

Zhang J G, Hu H J, Tian R, Chen Q L, Yang X P. Establishment of primary core collection of Pyrus pyrifolia germplasms in China. Hubei Agricultural Sciences, 2011, 50(8): 1590-1592. (in Chinese)

[13]刘勇, 孙中海, 刘德春, 吴波, 周群. 利用分子标记技术选择柚类核心种质资源. 果树学报, 2006, 23(3): 339-345.

Liu Y, Sun Z H, Liu D C, Wu B, Zhou Q. Screening the core collection of pomelo germplasm based on molecular marker. Journal of Fruit Science, 2006, 23(3): 339-345. (in Chinese)

[14]高志红, 章镇, 韩振海, 房经贵. 中国果梅核心种质的构建与检测. 中国农业科学, 2005, 38(2): 363 -368.

Gao Z H, Zhang Z, Han Z H, Fang J G. Development and evaluation of core collection of Japanese apricot germplasms in China. Scientia Agricultura Sinica, 2005, 38(2): 363-368. (in Chinese)

[15]郭大龙, 刘崇怀, 张君玉, 张国海. 葡萄核心种质的构建. 中国农业科学, 2012, 45(6): 1135-1143.

Guo D L, Liu C H, Zhang J Y, Zhang G H. Construction of grape core collections. Scientia Agricultura Sinica, 2012, 45(6): 1135-1143. (in Chinese)

[16]崔艳华, 邱丽娟, 常汝镇, 吕文河. 植物核心种质研究进展. 植物遗传资源学报, 2003, 4(3): 279-284.

Cui Y H, Qiu L J, Chang R Z, Lü W H. Advances in research on core collection of plant germplasm resources. Journal of Plant Genetic Resources, 2003, 4(3): 279-284. (in Chinese)

[17]Fjiellstrom R G, Parfitt D E. RFLP inheritance and linkage in walnut. Theoretical and Applied Genetics, 1994, 89: 665-670.

[18]Nicese F P, Hormaza J I, Mcgranahan G H. Molecular characterization and genetic relatedness among walnut (Juglans regia L.) genotype based on RAPD markers. Euphytica, 1998, 101: 199-206.

[19]Potter D, Gao F Y, Aiello G, Leslie C, Granahan G M. Intersimple sequence repeat markers for fingerprinting and determining genetic relationships of walnut(Juglans regia L.) cultivars. Journal of the American Society for Horticultural Science, 2002, 127(1): 75-81.

[20]Fornari B, Cannata F, Spada M, Malvolti M E. Allozyme analysis of genetic diversity and differentiation in European and Asiatic walnut Juglans regia L.) populations. Forest Genetics, 1999, 6(2): 115-127.

[21]Fornari B, Malvolti M E, Taurchini D, Fineschi S, Beritognolo I, Maccaglia E, Cannata F. Isozyme and organellar DNA analysis of genetic diversity in natural/naturalised European and Asiatic walnut (Juglans regia L.) populations. Acta Horticulturae, 2001, 544: 167-178.

[22]王滑, 郝俊民, 王宝庆, 裴东. 中国核桃8个天然居群遗传多样性分析. 林业科学, 2007, 43(7): 120-124.

Wang H, Hao J M, Wang B Q, Pei D. SSR analysis of genetic diversity of eight natural walnut populations in China. Scientia Silvae Sinicae, 2007, 43(7): 120-124. (in Chinese)

[23]Foroni I, Woeste K, Monti L M, Rao R. Identification of ‘Sorrento’walnut using simple sequence repeats (SSRs). Genetic Resources and Crop Evolution, 2007, 54: 1081-1094.

[24]Ciarmiello L F, Piccirillo P, Pontecorvo G, Luca A D, Kafantaris I, Woodrow P. A PCR based SNPs marker for specific characterization of English walnut (Juglans regia L.) cultivars. Molecular Biology Reports, 2011, 38(2): 1237-1249.

[25]Wang H, Zhao S, Zhang Z, Gao Y, Zhao Y, Fang J, He F. Genetic relationship and diversity of eight Juglans species in China estimated through AFLP analysis. Acta Horticulturae (ISHS), 2010, 861: 143-150.

[26]王红霞, 赵书岗, 高仪, 张志华, 玄立春. 普通核桃遗传多样性的AFLP分析. 中国农业科学, 2011, 44(7): 1434-1442.

Wang H X, Zhao S G, Gao Y, Zhang Z H, Xuan L C. Genetic diversity of Juglans regia L. cultivars revealed by AFLP analysis. Scientia Agricultura Sinica, 2011, 44(7): 1434-1442. (in Chinese)

[27]李自超, 张洪亮, 曾亚文, 杨忠义, 申时全, 孙传清, 王象坤. 云南地方稻种资源核心种质取样方案研究. 中国农业科学, 2000, 33(5): 1-7.

Li Z C, Zhang H L, Zeng Y W, Yang Z Y, Shen S Q, Sun C Q, Wang X K. Study on sampling schemes of core collection of local varieties of rice in Yunnan, China. Scientia Agricultura Sinica, 2000, 33(5): 1-7. (in Chinese)

[28]Tohme J, Gonzales D O, Beebe S, Duque M C. AFLP analysis of gene pools of a wild bean core collection. Crop Science, 1996, 36(5): 1375-1384.

[29]郗荣庭, 张毅萍. 中国果树志•核桃卷. 北京: 中国林业出版社, 1996: 94-171.

Xi R T, Zhang Y P. Flora of China Fruits Walnut. Beijing: China

Forestry Press, 1996: 94-171. (in Chinese)

[30]明军, 张启翔, 兰彦平. 梅花品种资源核心种质构建. 北京林业大学学报, 2005, 27(2): 65-69.

Ming J, Zhang Q X, Lan Y P. Core collection of Prunus mume Sieb.et Zucc. Journal of Beijing Forestry University, 2005, 27(2): 65-69. (in Chinese)

[31]Polignano G B, Uggenti P, Sxippa G. Diversity analysis and core collection formation in Bari faba bean germplasm. Plant Genetic Resources Newsletter, 2001, 125: 33-38.

[32]何余堂, 涂金星, 傅廷栋, 李殿荣, 陈宝元. 陕西省白菜型油菜核心种质的初步构建. 中国油料作物学报, 2002, 24(1): 6-9.

He Y T, Tu J X, Fu T D, Li D R, Chen B Y. Preliminary development of core collection of Brassica campestris in Shaanxi province. Chinese Journal of Oil Crop Sciences, 2002, 24(1): 6-9. (in Chinese)