中国农业科学

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p-香豆酸通过加速伤口处聚酚软木脂和木质素的沉积促进马铃薯块茎愈伤

梁伟1,朱亚同1,柴秀伟1,孔蕊1,李斌山1,李永才1毕阳1*Dov Prusky1,2
  

  1. 1甘肃农业大学食品科学与工程学院,甘肃 兰州 730070;2Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Rishon LeZion 7505101, Israel
  • 收稿日期:2020-12-18 接受日期:2021-04-25 发布日期:2021-05-25

p-coumaric acid promoted wound healing of potato tubers by accelerating the deposition of suberin poly phenolic and lignin at wound sites

LIANG Wei1, ZHU YaTong1, CHAI XiuWei1, KONG Rui1, LI BinShan1, LI YongCai1, BI Yang1*, Dov Prusky1,2 #br#   

  1. 1College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;  2Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Rishon LeZion 7505101, Israel
  • Received:2020-12-18 Accepted:2021-04-25 Online:2021-05-25

摘要: 【目的】研究p-CA处理对马铃薯块茎伤口处聚酚软木脂和木质素积累的影响及相关机理,促进马铃薯储藏技术发展。【方法】用0.5 mmol?L-1 p-CA浸泡切半‘大西洋’马铃薯块茎10 min,于室温条件下避光贮存进行愈伤。测定损伤块茎在愈伤期间的失重率以及损伤接种硫色镰刀菌(Fusarium sulphureum)块茎的病情指数,观察伤口处聚酚软木脂和木质素的积累,测定伤口处苯丙烷代谢关键酶和过氧化物酶活性,以及苯丙烷代谢产物和H2O2含量。【结果】p-CA处理显著降低了损伤块茎的失重率和损伤接种块茎的病情指数,14天时处理块茎的失重率和病情指数分别低于对照38.46%和43.18%。p-CA处理加速了块茎伤口处聚酚软木脂和木质素的积累,14 d时处理块茎的聚酚软木脂和木质素的细胞层厚度分别高出对照26.43%和30.26%。p-CA处理还明显提高了块茎伤口处苯丙氨酸解氨酶、肉桂酸-4-羟化酶、4-香豆酰辅酶A连接酶和肉桂醇脱氢酶的活性,7 d时处理块茎的酶活性分别高出对照32.17%、23.51%、25.09%和23.08%。p-CA处理还促进了伤口处肉桂酸、p-香豆酸、咖啡酸、阿魏酸、芥子酸和总酚的合成,14 d时处理块茎的肉桂酸、p-香豆酸、咖啡酸和总酚含量分别高出对照34.26、38.29%、19.31%和41.04%,21 d时处理块茎的阿魏酸、芥子酸含量分别高出对照38.33%和20.47%。此外,p-CA处理促进了块茎伤口处肉桂醇、松柏醇、芥子醇和木质素的积累,14 d时处理块茎的肉桂醇和木质素含量分别高出对照36.93%和31.66%,21 d时处理块茎的松柏醇和芥子醇含量分别高出对照41.43%和34.05%。p-CA处理还提高了块茎伤口处H2O2含量和过氧化物酶活性,7 d时处理块茎的H2O2含量和过氧化物酶活性分别高出对照40.25%和27.01%。【结论】p-CA处理可通过激活苯丙烷代谢,提高H2O2含量和过氧化物酶活性,促进马铃薯块茎伤口处聚酚软木脂和木质素的积累,从而加速块茎愈伤。

关键词: p-CA, 马铃薯块茎, 愈伤, 苯丙烷代谢

Abstract: 【Objective】This study aim to investigate the effect and related mechanism of p-CA treatment on the accumulation of suberin polyphenolic and lignin at wound sites of potato tubers.【Method】The half cut potato tubers ‘cv. Atlantic’ were soaked with 0.5 mmol?L-1 p-CA for 10 min and stored at room temperature and protected from light for healing. The weight loss and the disease index of wounded tubers inoculated with Fusarium sulphureum were determined, the deposition of suberin polyphenolic and lignin at wounded sites were observed, the activity of the key enzymes of phenylpropane metabolism and peroxidase as well as the content of phenylpropane metabolism products and H2O2 at wounded sites were measured.【Result】The weight loss and the disease index of wounded tubers were significantly reduced by p-CA treatment, and were 38.46% and 43.18% lower than those of control on the 14 day of healing, respectively. p-CA treatment accelerated the deposition of suberin polyphenolic and lignin at wound sites of potato tubers, and the thickness of cell layers in treated tubers were 26.43% and 30.26% higher than the control on the 14 day of healing, respectively. p-CA treatment also significantly increased the activities of phenylalanine ammonia lyase, cinnamic acid-4-hydroxylase, 4-coumaryl coenzyme A ligase and cinnamyl alcohol dehydrogenase at wounde sites of tubers, with the enzyme activities of the treated tubers being 32.17%, 23.51%, 25.09% and 23.08% higher than the control on 7 day, respectively. p-CA treatment also promoted the synthesis of cinnamic acid, p-coumaric acid, caffeic acid, ferulic acid, sinapic acid and total phenolics, with the contents of cinnamic acid, p-coumaric acid, caffeic acid and total phenolics in the treated tubers on 14 day being 34.26%, 38.29%, 19.31% and 41.04% higher than the control respectively, and the contents of ferulic acid, sinapic acid in the treated tubers being 38.33% and 20.47% higher than the control on 21 day, respectively. In addition, p-CA treatment promoted the accumulation of cinnamyl alcohol, coniferyl alcohol, sinapis alcohol and lignin at wound sites, with the contents of cinnamyl alcohol and lignin in the treated tubers being 36.93% and 31.66% higher than the control on 14 day, respectively, and the contents of coniferyl alcohol and sinapis alcohol in the treated tubers were 41.43% and 34.05% higher than the control on 21 day, respectively. The p-CA treatment also increased the H2O2 content and peroxidase activity at the wound sites, with the H2O2 content and peroxidase activity of the treated tubers being 40.25% and 27.01% higher than the control on 7 day, respectively.【Conclusion】The p-CA treatment accelerated wound healing of potato tubers by activating phenylpropanoid metabolism, increasing H2O2 content and peroxidase activity, and promoting the accumulation of suberin polyphenolic and lignin in potato tuber wound sites. 

Key words: p-CA, potato tuber, wound healing, phenylpropanoid metabolism