关键词: 五指山小型猪近交系, 白细胞抗原, 经典SLAⅠ3基因, RT-PCR

Abstract: To elucidate the structure of SLAⅠ3 alleles on inbred line of Wuzhishan pig（WZSP） population. RT-PCR products of the partial exon1, completed exon2 and partial exon3 of the SLA classical I class 3 gene were successed to amplify in 32 WZSPs. According to genealogy and amplification results, PCR products of 16 WZSPs were selected to clone and sequence. 10 different nucleotide sequences were obtained. The sequences data and their coden amino acids sequences were compared with all sequences of SLAⅠ3 gene in GenBank, respectively. It was found that these sequences were different in comparison with each other or with those of published sequences of SLAⅠ3 gene, but there were very few variation among them. The data of comparing the amino acids sequences indicated that most of these sites are conserved among the related molecules between porcine and human. Meanwhile, construction of a phylogenetic tree using the nucleotide sequences of those 23 alleles and 1 HLA A gene indicated that WZSP population own its unique resource.

Key words: Inbreding Wuzhishan pig, Leucocyte antigen, SLA classical I class 3 gene, RT-PCR