中国农业科学 ›› 2006, Vol. 39 ›› Issue (06): 1264-1271 .doi: 10.3864/j.issn.0578-1752.at-2005-6517

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

泰山螭霖鱼卵巢年周期中卵母细胞发育及其性腺类固醇激素作用

董玉兰,王树迎   

  • 收稿日期:2005-12-12 修回日期:1900-01-01 出版日期:2006-06-10 发布日期:2006-06-10

Study on the Oocyte Development and the Effect of Sex Steroid Hormone of Varicorhinus macrolepis on Reproductive Annual Cycle

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  • Received:2005-12-12 Revised:1900-01-01 Published:2006-06-10 Online:2006-06-10

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被引次数

18

摘要： 【目的】研究泰山螭霖鱼(Varicorhinus macrolepis)卵巢和卵母细胞在年周期中的发育变化规律，以及性激素在卵母细胞发育中的调控作用；【方法】利用解剖学、组织学、组织化学以及放射免疫的方法对泰山螭霖鱼卵巢和卵母细胞的解剖学、组织学、组织化学以及性激素水平在年周期中的变化规律进行了研究。【结果】2～4月份，卵巢由Ⅳ期发育至第v期，4月份进入繁殖期；4～6月份，为第v期；6月下旬～7月份为第vi期，是排卵后退化阶段；8月份，为Ⅱ期卵巢（休整期）；9月份～翌年的1月份为第Ⅲ期，以Ⅲ期卵巢过冬。泰山螭霖鱼血清17β-雌二醇(E2)自1月份开始升高，排卵前即3月份达到高峰（53.6 pg·ml-1左右），4月份排卵时降为20.22 pg·ml-1，一直持续到9月份，降至最低点，1.6667 pg·ml-1左右，之后维持在低水平，到1月份又开始上升。血清17α，20β－双羟孕酮（17α，20β-DHP）在4月份（接近排卵时）达到高峰，56.333 pg·ml-1，然后维持高水平（56.333～50.667 pg·ml-1）至排卵结束（6月份），11月份～翌年3月份均维持低水平（35～15 pg·ml-1）；【结论】泰山螭霖鱼为短期分批产卵类型，一年一个产卵期，E2和17α，20β-DHP对卵母细胞发育有重要的调控作用。

关键词: 泰山螭霖鱼, 卵巢, 卵母细胞, 性腺类固醇激

Abstract: 【Objective】To investigate the development of ovary and oocytes of Varicorhinus macrolepis, and the effect of sex hormone on development of ovary. 【Method】Anatomy, histology, histochemistry of the oocytes were studied and the changes of sexual steroid by Radioimmunoassay(RIA) in annual reproductive cycle were also studied in the study; 【Result】The results are as follows: (Ⅰ) from February to April, the ovary developed from phase Ⅳ to phase Ⅴ; (Ⅱ) from April to June, the ovary was in phase Ⅴ; (Ⅲ) from June to July, after the finish of ovulation, the ovary entered phase Ⅵ. (Ⅳ) in August, the ovary changed into phase Ⅱagain; (Ⅴ) from September to January next year, ovary was in Ⅲ period and spent cold in winter. The concentration of E2 went up to peak in March before ovulating (53.6 pg·ml-1 around), and began to drop to minimum point from April to September and maintained at low level until January, then began to rise to March. The concentration of 17α, 20β-DHP rose to the highest point in April, and maintained at high level to the end of ovulating. Then it decreased from July to November and maintained at low level until March of next year; 【Conclusion】The results indicated that Varicorhinus macrolepis is in a successive process, and spawning season only once a year. The modulation of E2 and 17α, 20β-DHP was sufficient to the oocytes development in Varicorhinus macrolepis.

Key words: Varicorhinus macrolepis, Ovary, Oocyte, Goandal steroid hormone

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