关键词: 干扰素（IFN）, 成熟蛋白, 原核表达, 真核表达, 生物学活性

Abstract: the IFN genes, which encode goose interferon γ mature protein, were cloned from total RNA was derived from PHA-stimulated-cultured (PBMCs) of goose by reverse transcription polymerrase chain reaction (RT-PCR). The amplified products of PCR were cloned into pMD18-T vector and sequenced. Compared with the published goose IFN-γ genes sequence, the homology of nucleotide sequence were 100.00%. The fragments of IFN-γ were subcloned into plasmid pET30a and transfer plasmid pMelBacA, pBlueBacHis2A, respectively. the purified recombinant baculovirus were obtained. goose interferon γ mature proteins were successfully expressed in the prokaryotic and eukaryotic expression systems, respectively. And prepared polyclonal antibodies against the products of the prokaryotic expression. There have the common antigenic between the products of prokaryotic and eukaryotic expression systems. One of the biological activity of recombinant mature goose IFN-γ is the ability of activating macrophage of goose to generate nitric oxide(NO) was detected in vitro.

Key words: Interferon(IFN), mature protein, prokaryotic expression, eukaryotic expression, biological activity