关键词: 家蚕, eIF2α, 荧光差异显示, 生物信息学分析, 酪氨酸硫酸盐化

Abstract: 【Objective】Clone full cDNA sequence of Bombyx mori eIF2α, analyse cite mutation between susceptible and resistant strain infected by Bombyx mori densovirus-Z (BmDNV-Z). 【Method】Using fluorescent differential display (FDD) technique, a special band named G13-1b650 was obtained in highly susceptible strain HUABA infected by Bombyx mori densovirus-Z (BmDNV-Z). Then electrically extended and further confirmed by using specific primers, a full cDNA sequence of Bombyx mori eIF2α（BmeIF2α）gene was cloned. 【Result】The full length of the obtained cDNA sequence was 1 100 bp (GenBank accession number: DQ073458). Its open reading frame (ORF) includes 999 bp and encodes 332 amino acid residues. The deduced amino acid sequence showed 94.3% identity to eIF2α of Spodoptera frugiperda. It contains one putative conservative phosphorylation site (S（51）R（52）R（52）R（54）R（56）K（60）R（63）), three phosphorylation sites of Protein kinase C, five phosphorylation sites of Tyrosinase, nine phosphorylation sites of Tyrosinase Ⅱ, three phosphorylation sites of protein kinase depended on cAMP or cGMP, two tyrosine sulphation sites and one glycosylation site. 【Conclusion】BmeIF2α in highly susceptible strain contains one tyrosine sulphation site by analyzing sequences from 113-127, H V A E L L H Y E T S E Q S E. While in resistant strains, a C372 to T mutation which leads Serine126 mutaton to Leucine results in the absence of the tyrosine sulphation site in the sequences from 113-127.

Key words: Bombyx mori, eIF2α, FDD, Bioinformatical analysis, Tyrosine-sulfated