关键词: 人工合成小麦, 圆锥小麦, 节节麦, LMW-GS基因, 序列分析

Abstract: RSP is a synthetic hexaploid wheat (Triticum turgidum-Aegilops tauschii) between a tetraploid wheat (T. turgidum L.) and an Ae. tauschii accession with highly tolerant to sprouting, which is of importance to maintain wheat flour making quality. LMW-GSs together with HMW-GSs, control wheat flour making quality directly. To investigate the LMW-GSs from RSP, using PCR amplification of the genomic DNA, three novel LMW-GS genes (i.e. LMWRSP-1, LMWRSP-2 and LMWRSP-3) were isolated from 'RSP', among which LMWRSP-2 (GenBank accession number: AY676682) is a pseudogene due to one stop code in its coding regions. The coding regions of LMWRSP-1 (GenBank Accession number: AY676681) and LMWRSP-3 (GenBank accession number: AY676683) are 825 and 915 bp, respectively. They encode two LMW glutenin subunits with 274 and 304 amino acid residues, respectively. Multiple sequence alignment analysis indicated that LMWRSP-1 and LMWRSP-3 have the highest similarity scores to the known LMW-GS genes encoded by Glu-A3 and Glu-D3, respectively.

Key words: Synthetic hexaploid wheat, Triticum turgidum, Aegilops tauschii, LMW-GS gene, Sequence analyses