最新录用：四倍体小麦胚大小性状QTL定位与分析

doi:10.3864/j.issn.0578-1752.220562

摘要/Abstract

摘要： 【目的】挖掘小麦胚大小性状相关的数量性状位点，解析胚大小与其他重要农艺性状之间的相关性，为胚相关性状QTL的精细定位及育种利用奠定基础。【方法】以四倍体小麦矮兰麦（Ailanmai）和野生二粒小麦（LM001）构建的121份F₈代重组自交系群体（AM群体）作为研究材料，将其分别种植于成都市温江区试验基地（2018、2019和2020年）、成都市崇州试验基地（2020年）和雅安市试验基地（2020年），调查5个环境下的胚长、胚宽、胚长/胚宽、胚长/粒长、胚宽/粒宽以及胚面积6个性状，结合基于小麦55K SNP芯片构建的遗传连锁图谱对上述6个性状进行QTL定位。【结果】胚大小性状呈近似正态分布，符合数量性状的遗传特征。QTL定位共检测到27个胚大小相关性状的QTL，其中，7个分别控制胚长和胚宽的QTL可解释11.88%—18.99%和21.77%—29.41%的表型变异，共检测到5个在多环境稳定表达的主效QTL：QEL.sicau-AM-3B、QEW.sicau-AM-2B、QEW/KW.sicau-AM-2B、QEL/EW.sicau-AM-2B-1和QEA.sicau-AM-2B，其贡献率分别为11.88%—21.74%、21.77%—33.29%、8.80%—24.92%、12.79%—31.13%和10.47%—20.67%。另外，上述胚相关的位点形成4个QTL簇，分别为1B控制胚长/粒长和胚长的QTL簇，2B控制胚宽、胚长/胚宽、胚宽/粒宽以及胚面积的QTL簇，3B控制胚长和胚面积的QTL簇，6B控制胚长/胚宽、胚宽/粒宽的QTL簇。确定胚大小与小麦籽粒大小具有显著正相关性，且发现胚长主效位点QEL.sicau-AM-3B与粒长主效位点共定位，但胚宽主效位点QEW.sicau-AM-2B独立于粒宽主效位点存在。在主效QTL所在物理区间鉴定获得4个可能参与胚大小调控的基因。【结论】鉴定到5个控制胚相关性状的稳定表达的主效QTL——QEL.sicau-AM-3B、QEW.sicau-AM-2B、QEW/KW.sicau-AM-2B、QEL/EW.sicau-AM-2B-1和QEA.sicau-AM-2B，其中，QEW.sicau-AM-2B可能为新的QTL。

关键词: 四倍体小麦, 胚长, 胚宽, 胚面积, QTL定位

Abstract: 【Objective】This study is to excavate embryo-related quantitative trait loci (QTL) with potential breeding value, to explore the genetic relationship between embryo and other agronomic traits in tetraploid wheat, and finally to aim at laying an important foundation for the fine mapping and breeding utilization of embryo-related traits in the future.【Method】A total of 121 F₈ recombinant inbred lines (RIL) constructed by crossing tetraploid durum wheat (Ailanmai) and wild emmer wheat (LM001) were used. This RIL population was planted in five different environments including Wenjiang (2018-2020), Chongzhou (2020), and Ya'an (2020) in Sichuan Province for phenotypic evaluation of embryo length (EL), embryo width (EW), embryo length/embryo width (EL/EW), embryo length/kernel length (EL/KL), embryo width/kernel width (EW/KW), and embryo area (EA). QTL mapping was performed based on a genetic linkage map constructed based on the wheat 55K SNP. 【Result】 The embryo size-related traits showed approximately normal distribution in the RIL population satisfying the genetic characteristics of quantitative traits. A total of 27 QTL for embryo size-related traits were detected in five environments over three years. Among them, seven ones controlling EL could contribute 11.88 % to 18.99% of phenotypic variation. Seven QTLs controlling EW could explain 21.77 to 29.41% of phenotypic variation. Five stable and major QTLs (QEL.sicau-AM-3B, QEW.sicau-AM-2B, QEW/KW.sicau-AM-2B, QEL/EW.sicau-AM-2B-1 and QEA.sicau-AM-2B) were identified, and they explained 11.88% to 18.99%, 21.77% to 29.41%, 8.80% to 24.92%, 12.79% to 25.69% and 10.47% to 15.22% of phenotypic variation, respectively. In addition, four QTL-rich regions were identified in the embryo size-related loci mentioned above. The QTL controlling EL/KL and EL was located on chromosome 1B, that for EW, EL/EW, EW/KW, and EA was located on 2B, that controlling EL and EA was on 3B, and that controlling EL/EW and EW/KW was on 6B. Embryo size was significantly and positively correlated with kernel size. Further, the major QTL for EL, QEL.sicau-AM-3B was co-located with that for kernel length identified previously, but that for EW QEW.sicau-AM-2B was independent of that for kernel width. Four genes likely involved in regulation of embryo size were identified in intervals where major QTL were mapped. 【Conclusion】Five stable and major QTLs were identified: QEL.sicau-AM-3B, QEW.sicau-AM-2B, QEW/KW.sicau-AM-2B, QEL/EW.sicau-AM-2B-1, QEA.sicau-AM-2B, among which QEW.sicau-AM-2B may be novel.

Key words: tetraploid wheat, embryo length, embryo width, embryo area, QTL mapping

陈吉浩, 周界光, 曲翔汝, 王素容, 唐华苹, 蒋云, 唐力为, 兰秀锦, 魏育明, 周景忠, 马建. 最新录用：四倍体小麦胚大小性状QTL定位与分析[J]. 中国农业科学, 0, (): 1-.

CHEN JiHao, ZHOU JieGuang, QU XiangRu, WANG SuRong, TANG HuaPing, JIANG Yun, TANG LiWei, LAN XiuJin, WEI YuMING, ZHOU JingZhong, MA Jian. Mapping and Analysis of QTL for Embryo Size-Related Traits in Tetraploid Wheat[J]. Scientia Agricultura Sinica, 0, (): 1-.

[1]	贾晓昀, 王士杰, 朱继杰, 赵红霞, 李妙, 王国印. 陆地棉高密度遗传图谱的构建及产量相关性状的QTL定位[J]. 中国农业科学, 2023, 56(4): 587-598.
[2]	陈吉浩, 周界光, 曲翔汝, 王素容, 唐华苹, 蒋云, 唐力为, $\boxed{\hbox{兰秀锦}}$, 魏育明, 周景忠, 马建. 四倍体小麦胚大小性状QTL定位与分析[J]. 中国农业科学, 2023, 56(2): 203-216.
[3]	刘进,胡佳晓,马小定,陈武,勒思,Jo Sumin,崔迪,周慧颖,张立娜,Shin Dongjin,黎毛毛,韩龙植,余丽琴. 水稻RIL群体高密度遗传图谱的构建及苗期耐热性QTL定位[J]. 中国农业科学, 2022, 55(22): 4327-4341.
[4]	邹林翰,周新颖,张泽源,蔚睿,袁梦,宋晓朋,简俊涛,张传量,韩德俊,宋全昊. 小麦周8425B×小偃81重组自交系群体千粒重相关性状的QTL定位及单倍型分析[J]. 中国农业科学, 2022, 55(18): 3473-3483.
[5]	常立国,何坤辉,刘建超. 多环境下玉米保绿相关性状遗传位点的挖掘[J]. 中国农业科学, 2022, 55(16): 3071-3081.
[6]	张亚东,梁文化,赫磊,赵春芳,朱镇,陈涛,赵庆勇,赵凌,姚姝,周丽慧,路凯,王才林. 水稻RIL群体高密度遗传图谱构建及粒型QTL定位[J]. 中国农业科学, 2021, 54(24): 5163-5176.
[7]	王彦,范保杰,曹志敏,张志肖,苏秋竹,王珅,王学清,彭秀国,梅丽,武玉华,刘少兴,田胜民,徐俊杰,蒋春志,王伟娟,刘长友,田静. 基于新遗传连锁图谱的豇豆抗豆象QTL定位[J]. 中国农业科学, 2021, 54(22): 4740-4749.
[8]	渠可心,韩露,谢建国,潘文婧,张泽鑫,辛大伟,刘春燕,陈庆山,齐照明. 基于RIL和CSSL群体定位大豆脂肪酸组分QTL[J]. 中国农业科学, 2021, 54(15): 3168-3182.
[9]	张健,杨靖,王豪,李冬秀,杨瑰丽,黄翠红,周丹华,郭涛,陈志强,王慧. 基于高密度遗传图谱定位水稻籽粒大小相关性状QTL[J]. 中国农业科学, 2020, 53(2): 225-238.
[10]	王琴,刘泽厚,万洪深,魏会廷,龙海,李涛,邓光兵,李俊,杨武云. 川麦42和川农16抗穗发芽QTL定位及聚合效应分析[J]. 中国农业科学, 2020, 53(17): 3421-3431.
[11]	牛志红,宋晓飞,李晓丽,郭晓雨,何书强,贺栾劲芝,冯志红,孙成振,闫立英. 黄瓜单性结实性状遗传与QTL定位[J]. 中国农业科学, 2020, 53(1): 160-171.
[12]	陈静静, 刘谢香, 于莉莉, 卢一鹏, 张嗣天, 张昊辰, 关荣霞, 邱丽娟. 利用BSA法发掘野生大豆种子硬实性相关QTL[J]. 中国农业科学, 2019, 52(13): 2208-2219.
[13]	王兵伟, 覃嘉明, 时成俏, 郑加兴, 覃永嫒, 黄安霞. 一个高抗玉米南方锈病基因的QTL定位及遗传分析[J]. 中国农业科学, 2019, 52(12): 2033-2041.
[14]	丁膺宾, 张莉珍, 许睿, 王艳艳, 郑晓明, 张丽芳, 程云连, 吴凡, 杨庆文, 乔卫华, 兰进好. 基于染色体片段置换系的野生稻粒长QTL——qGL12的精细定位[J]. 中国农业科学, 2018, 51(18): 3435-3444.
[15]	马亚茹，冯建灿，刘崇怀，樊秀彩，孙海生，姜建福，张颖. 葡萄无核性状的SSR新分子标记开发及应用[J]. 中国农业科学, 2018, 51(13): 2622-2630.

最新录用：四倍体小麦胚大小性状QTL定位与分析

Mapping and Analysis of QTL for Embryo Size-Related Traits in Tetraploid Wheat

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0