球孢白僵菌与食诱剂联合应用对番茄上烟粉虱的防治研究

doi:10.3864/j.issn.0578-1752.2026.12.008

摘要/Abstract

摘要：

【目的】在室内与田间条件下系统评估球孢白僵菌（Beauveria bassiana）对烟粉虱（Bemisia tabaci）的致病力、食诱剂对烟粉虱的引诱率，以及两者联合应用对烟粉虱的综合防治效果，筛选高致病力球孢白僵菌菌株与高效食诱剂配方。【方法】室内条件下，采用浸叶法和接触法分别测定球孢白僵菌菌株对烟粉虱若虫和成虫的致病力，并采用血球计数板法测定各菌株产孢量。利用Y型管趋性装置分析烟粉虱对植物源挥发物的趋性；并通过组分、配比、浓度、溶剂和缓释载体试验确定复合食诱剂的最优配方。在田间构建球孢白僵菌-食诱剂联合装置，验证装置悬挂位置和密度对烟粉虱诱集效果的影响；通过室内笼罩试验和田间调查测定烟粉虱孢子携带量及种群的动态变化。【结果】在1×10⁸孢子/mL浓度条件下，XJWLMQ-1菌株对烟粉虱2龄若虫和成虫的校正死亡率分别为93.16%和79.17%，致死中时（LT₅₀）分别为3.78和7.12 d；在产孢培养基上产孢量达3.74×10⁸孢子/cm²。顺-3-己烯醛、芳樟醇和丁香酚按1﹕2﹕1比例组合形成的复合食诱剂诱集效果最佳，引诱率达90.0%。液体石蜡与正己烷混合溶剂、配合多孔硅胶棒作为缓释载体，表现出良好的持效性，在84 d时仍维持60.0%的引诱率；食诱剂装置悬挂于植株顶端位置诱捕数量显著高于中部及顶部以上20 cm处。笼罩试验表明，烟粉虱可通过接触联合应用装置获取并携带（1.50—4.34）×10⁴孢子/头。在14 d内，虫体孢子携带量随暴露时间延长持续增加，在第14天达到峰值，为1.40×10⁵孢子/头。连续两年田间试验显示，处理组的烟粉虱成虫峰值分别为16.40和15.60头/叶，较对照组分别下降36.3%和37.6%（P<0.05）。【结论】筛选出对烟粉虱若虫和成虫均具高致病力且产孢量高的球孢白僵菌XJWLMQ-1菌株，复配出对烟粉虱引诱率显著的食诱剂配方(顺-3-己烯醛﹕芳樟醇﹕丁香酚为1﹕2﹕1)，球孢白僵菌和食诱剂联合应用对烟粉虱具有良好的防控效果，可持续抑制烟粉虱种群增长，具备推广应用潜力。

关键词: 球孢白僵菌, 食诱剂, 烟粉虱, 综合防治

Abstract:

【Objective】The objective of this study was to systematically evaluate the pathogenicity of Beauveria bassiana to Bemisia tabaci, the attraction rate of food-based attractants to B. tabaci, and the synergistic efficacy of their combination against B. tabaci under both laboratory and field conditions, and to screen the highly pathogenic B. bassiana strains and optimized attractant formulations for effective whitefly control.【Method】Under controlled laboratory conditions, the pathogenicity of multiple B. bassiana strains against nymphal and adult stages of B. tabaci was assessed using leaf-dip and contact bioassays, respectively. Conidial production per unit area was quantified microscopically using a hemocytometer. Behavioral responses of B. tabaci to plant-derived volatile organic compounds (VOCs) were evaluated in a Y-tube olfactometer assay. An optimized food-based attractant formulation was developed through systematic screening of active components, empirical optimization of component ratios and concentrations, evaluation of solvent compatibility, and assessment of slow-release carrier efficacy. A prototype dual-function device—integrating the selected B. bassiana strain with the optimized attractant—was designed for field deployment. The influence of device hanging height and inter-unit spacing on whitefly attractive effect was rigorously tested under semi-field and field conditions. Finally, conidial acquisition by B. tabaci individuals and subsequent population-level impacts were quantified via standardized cage experiments and longitudinal field monitoring.【Result】At a concentration of 1×10⁸ conidia/mL, strain XJWLMQ-1 caused corrected mortalities of 93.16% and 79.17% against second-instar nymphs and adults of B. tabaci, respectively, with LT₅₀ values of 3.78 and 7.12 d. The conidial production of this strain reached 3.74×10⁸ conidia/cm² on sporulation medium. A compound attractant consisting of (Z)-3-hexenal, linalool, and eugenol at a ratio of 1﹕2﹕1 showed the highest attraction rate (90.0%). A mixed solvent of liquid paraffin and n-hexane combined with a porous silica gel rod as a slow-release carrier exhibited good persistence, maintaining an attraction rate of 60.0% after 84 d. Devices suspended at the top of tomato plants attracted significantly more whiteflies than those placed at the middle of plants or 20 cm above the canopy. Cage experiments showed that B. tabaci could acquire and carry (1.50-4.34) ×10⁴ conidia per adult through contact with the combined application device. Conidial loads carried by adults increased with exposure time and reached a peak of 1.40×10⁵ conidia per adult after 14 d. Two years of field trials demonstrated that the peak densities of B. tabaci adult in the treatment plots were 16.40 and 15.60 individuals per leaf, representing reductions of 36.3% and 37.6%, respectively, compared with the control (P<0.05).【Conclusion】A highly pathogenic B. bassiana strain XJWLMQ-1 against both nymphs and adults of B. tabaci was screened, and an efficient food attractant formulation consisting of (Z)-3-hexenal, linalool, and eugenol at a ratio of 1﹕2﹕1 was developed. The combined application of B. bassiana and the attractant effectively suppressed B. tabaci populations and showed strong potential for sustainable field application.

Key words: Beauveria bassiana, food attractant, Bemisia tabaci, combined control

王萌涵, 王登杰, 梁兴慧, 王恩东, 张博, 王海鸿. 球孢白僵菌与食诱剂联合应用对番茄上烟粉虱的防治研究[J]. 中国农业科学, 2026, 59(12): 2637-2655.

WANG MengHan, WANG DengJie, LIANG XingHui, WANG EnDong, ZHANG Bo, WANG HaiHong. Control of Bemisia tabaci on Tomato by the Combined Application of Beauveria bassiana and Food Attractants[J]. Scientia Agricultura Sinica, 2026, 59(12): 2637-2655.

图/表 14

表1

图1

表2

表3

图2

图3

图4

图5

表4

表5

图6

图7

图8

图9

参考文献 63

[1]	DE BARRO P J, LIU S S, BOYKIN L M, DINSDALE A B. Bemisia tabaci: A statement of species status. Annual Review of Entomology, 2011, 56: 1-19. doi: 10.1146/annurev-ento-112408-085504 pmid: 20690829
[2]	LIU S S, DE BARRO P J, XU J, LUAN J B, ZANG L S, RUAN Y M, WAN F H. Asymmetric mating interactions drive widespread invasion and displacement in a whitefly. Science, 2007, 318(5857): 1769-1772. doi: 10.1126/science.1149887
[3]	HOROWITZ A R, GHANIM M, RODITAKIS E, NAUEN R, ISHAAYA I. Insecticide resistance and its management in Bemisia tabaci species. Journal of Pest Science, 2020, 93(12): 893-910. doi: 10.1007/s10340-020-01210-0
[4]	张帆, 李姝, 肖达, 赵静, 王然, 郭晓军, 王甦. 中国设施蔬菜害虫天敌昆虫应用研究进展. 中国农业科学, 2015, 48(17): 3463-3476. doi: 10.3864/j.issn.0578-1752.2015.17.013.
	ZHANG F, LI S, XIAO D, ZHAO J, WANG R, GUO X J, WANG S. Progress in pest management by natural enemies in greenhouse vegetables in China. Scientia Agricultura Sinica, 2015, 48(17): 3463-3476. doi: 10.3864/j.issn.0578-1752.2015.17.013. (in Chinese)
[5]	王恩东, 闫红, 张博, 徐学农. 捕食螨在蔬菜生产中的应用. 中国生物防治学报, 2025, 41(6): 1494-1508. doi: 10.16409/j.cnki.2095-039x.2025.11.012
	WANG E D, YAN H, ZHANG B, XU X N. Application of predatory mite in vegetable production. Chinese Journal of Biological Control, 2025, 41(6): 1494-1508. (in Chinese) doi: 10.16409/j.cnki.2095-039x.2025.11.012
[6]	SKINNER M, PARKER B L, KIM J S. Role of entomopathogenic fungi// Integrated Pest Management. Amsterdam: Elsevier, 2014: 169-191.
[7]	SAIN S K, MONGA D, KUMAR R, NAGRALE D T, KRANTHI S, KRANTHI K R. Comparative effectiveness of bioassay methods in identifying the most virulent entomopathogenic fungal strains to control Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Egyptian Journal of Biological Pest Control, 2019, 29(1): 31. doi: 10.1186/s41938-019-0130-z
[8]	BOHATÁ A, FOLORUNSO E A, LENCOVÁ J, OSBORNE L S, MRAZ J. Control of sweet potato whitefly (Bemisia tabaci) using entomopathogenic fungi under optimal and suboptimal relative humidity conditions. Pest Management Science, 2024, 80(3): 1065-1075. doi: 10.1002/ps.v80.3
[9]	SAIN S K, MONGA D, KRANTHI S, HIREMANI N S, NAGRALE D T, KUMAR R, VERMA S K, PRASAD Y G. Evaluation of the bioefficacy and insecticide compatibility of entomopathogens for management of whitefly (Hemiptera: Aleyrodidae) on upland cotton under laboratory and polyhouse conditions. Neotropical Entomology, 2022, 51(4): 600-612. doi: 10.1007/s13744-022-00964-9 pmid: 35680781
[10]	EYAL J, MABUD M A, FISCHBEIN K L, WALTER J F, OSBORNE L S, LANDA Z. Assessment of Beauveria bassiana Nov. EO-1 strain, which produces a red pigment for microbial control. Applied Biochemistry and Biotechnology, 1994, 44: 65-80. doi: 10.1007/BF02921852
[11]	JARONSKI S T. Ecological factors in the inundative use of fungal entomopathogens. BioControl, 2010, 55(1): 159-185. doi: 10.1007/s10526-009-9248-3
[12]	EKESI S, MANIANIA N K, LWANDE W. Susceptibility of the legume flower thrips to Metarhizium anisopliae on different varieties of cowpea. BioControl, 2000, 45(1): 79-95. doi: 10.1023/A:1009927302916
[13]	HAJEK A E, LEGER R J. Interactions between fungal pathogens and insect hosts. Annual Review of Entomology, 1994, 39: 293-322. doi: 10.1146/ento.1994.39.issue-1
[14]	VEGA F E, DOWD P F, BARTELT R J. Dissemination of microbial agents using an autoinoculating device and several insect species as vectors. Biological Control, 1995, 5(4): 545-552. doi: 10.1006/bcon.1995.1064
[15]	OPISA S, DU PLESSIS H, AKUTSE K S, FIABOE K K M, EKESI S. Horizontal transmission of Metarhizium anisopliae between Spoladea recurvalis (Lepidoptera: Crambidae) adults and compatibility of the fungus with the attractant phenylacetaldehyde. Microbial Pathogenesis, 2019, 131: 197-204. doi: 10.1016/j.micpath.2019.04.010
[16]	刘胜, 刘召, 王帅宇, 张起恺, 雷仲仁, 王海鸿. 球孢白僵菌与食诱剂对西花蓟马的联合作用. 中国生物防治学报, 2023, 39(2): 297-304. doi: 10.16409/j.cnki.2095-039x.2022.03.017
	LIU S, LIU Z, WANG S Y, ZHANG Q K, LEI Z R, WANG H H. Combined use of Beauveria bassiana and food lure for control of Frankliniella occidentalis. Chinese Journal of Biological Control, 2023, 39(2): 297-304. (in Chinese)
[17]	PARADZA V M, KHAMIS F M, YUSUF A A, SUBRAMANIAN S, AKUTSE K S. Efficacy of Metarhizium anisopliae and (E)-2-hexenal combination using autodissemination technology for the management of the adult greenhouse whitefly, Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae). Frontiers in Insect Science, 2022, 2: 991336. doi: 10.3389/finsc.2022.991336
[18]	YASUDA K. Auto-infection system for the sweet potato weevil, Cylas formicarius (Fabricius) (Coleoptera: Curculionidae) with entomopathogenic fungi, Beauveria bassiana using a modified sex pheromone trap in the field. Applied Entomology and Zoology, 1999, 34(4): 501-505. doi: 10.1303/aez.34.501
[19]	BENVENUTI C, BARZANTI G P, MARIANELLI L, PEVERIERI G S, PAOLI F, BOSIO G, VENANZIO D, GIACOMETTO E, ROVERSI P F. A new device for auto-disseminating entomopathogenic fungi against Popillia japonica: A study case. Bulletin of Insectology, 2019, 72(2): 219-225.
[20]	蔡晓明, 李兆群, 潘洪生, 陆宴辉. 植食性害虫食诱剂的研究与应用. 中国生物防治学报, 2018, 34(1): 8-35. doi: 10.16409/j.cnki.2095-039x.2018.01.002
	CAI X M, LI Z Q, PAN H S, LU Y H. Research and application of food-based attractants of herbivorous insect pests. Chinese Journal of Biological Control, 2018, 34(1): 8-35. (in Chinese) doi: 10.16409/j.cnki.2095-039x.2018.01.002
[21]	曹凤勤, 刘万学, 范中南, 万方浩, 程立生. B型烟粉虱对三种寄主植物及其挥发物的行为反应. 昆虫学报, 2008, 51(8): 830-838.
	CAO F Q, LIU W X, FAN Z N, WAN F H, CHENG L S. Behavioural responses of Bemisia tabaci B-biotype to three host plants and their volatiles. Acta Entomologica Sinica, 2008, 51(8): 830-838. (in Chinese)
[22]	陈文斌. 烟粉虱对三种寄主植物的选择性及植株挥发物对其选择行为的影响[D]. 扬州: 扬州大学, 2021.
	CHEN W B. The selectivity of Bemisia tabaci on three host plants and the influence of plant volatiles on its selection behavior[D]. Yangzhou: Yangzhou University, 2021. (in Chinese)
[23]	李姝, 赵静, 张晓曼, 王文君, 罗晨, 王甦. 烟粉虱MED隐种对13种植物挥发性物质的行为反应. 植物保护学报, 2016, 43(1): 105-110.
	LI S, ZHAO J, ZHANG X M, WANG W J, LUO C, WANG S. Behavioral responses of Bemisia tabaci MED to 13 plant volatiles. Journal of Plant Protection, 2016, 43(1): 105-110. (in Chinese)
[24]	CHEN G, SU Q, SHI X, LIU X, PENG Z, ZHENG H, XIE W, XU B, WANG S, WU Q, ZHOU X, ZHANG Y. Odor, not performance, dictates Bemisia tabaci’s selection between healthy and virus infected plants. Frontiers in Physiology, 2017, 8: 146.
[25]	杨苗苗, 李英梅, 张淑莲, 洪波, 王晶玲, 陈志杰. 烟粉虱MED隐种对植物挥发物的趋性行为. 中国生物防治学报, 2018, 34(5): 663-669. doi: 10.16409/j.cnki.2095-039x.2018.05.003
	YANG M M, LI Y M, ZHANG S L, HONG B, WANG J L, CHEN Z J. Behavioral responses of Bemisia tabaci MED cryptic species to plant volatiles. Chinese Journal of Biological Control, 2018, 34(5): 663-669. (in Chinese)
[26]	GAFFKE A M, MILLER N W, SHARMA A, ALLAN S A. Attraction of sweet potato whitefly Bemisia tabaci (Hemiptera: Aleyrodidae), and two generalist predators to green leaf volatile compounds. Insects, 2024, 15(10): 750. doi: 10.3390/insects15100750
[27]	LI Y F, ZHONG S T, QIN Y C, ZHANG S Q, GAO Z L, DANG Z H, PAN W L. Identification of plant chemicals attracting and repelling whiteflies. Arthropod-Plant Interactions, 2014, 8: 183-190. doi: 10.1007/s11829-014-9302-7
[28]	FURLONG E. The Trojan ant: Infecting aphids with the fungus V. lecani using the ant Lasius niger[D]. Wye: Imperial College of Science, Technology and Medicine, 2002.
[29]	SREI N, GUERTIN C, LAVALLÉE R, LAJOIE M È, BROUSSEAU C, BERGEVIN R, MILLER F, MCMILLIN K, TRUDEL R. Microbial control of the emerald ash borer (Coleoptera: Buprestidae) using Beauveria bassiana (Hypocreales: Cordycipitaceae) by the means of an autodissemination device. Journal of Economic Entomology, 2020, 113(6): 2657-2665. doi: 10.1093/jee/toaa226
[30]	王登杰, 臧连生, 张烨, 王海鸿, 雷仲仁. 球孢白僵菌对烟粉虱后代生命表参数的亚致死影响. 中国农业科学, 2014, 47(18): 3588-3595. doi: 10.3864/j.issn.0578-1752.2014.18.007.
	WANG D J, ZANG L S, ZHANG Y, WANG H H, LEI Z R. Sublethal effects of Beauveria bassiana Balsamo on life table parameters of subsequent generations of Bemisia tabaci Gennadius. Scientia Agricultura Sinica, 2014, 47(18): 3588-3595. doi: 10.3864/j.issn.0578-1752.2014.18.007. (in Chinese)
[31]	VICENTINI S, FARIA M, DE OLIVEIRA M R V. Screening of Beauveria bassiana (Deuteromycotina: Hyphomycetes) isolates against nymphs of Bemisia tabaci biotype B (Hemiptera: Aleyrodidae) with description of a new bioassay method. Neotropical Entomology, 2001, 30(1): 97-103. doi: 10.1590/S1519-566X2001000100015
[32]	DIMBI S, MANIANIA N K, LUX S A, EKESI S, MUEKE J K. Pathogenicity of Metarhizium anisopliae (Metsch.) Sorokin and Beauveria bassiana (Balsamo) Vuillemin to three adult fruit fly species: Ceratitis capitata (Weidemann), C. rosa var. fasciventris Karsch and C. cosyra (Walker) (Diptera: Tephritidae). Mycopathologia. 2003, 156: 375-382. doi: 10.1023/B:MYCO.0000003579.48647.16
[33]	侯茂林, 文吉辉, 卢伟. 烟粉虱成虫在日光温室内的分布和日活动规律. 生态学报, 2006, 26(5): 1431-1437.
	HOU M L, WEN J H, LU W. Distribution and diurnal activity patterns of Bemisia tabaci adults in solar greenhouses. Acta Ecologica Sinica, 2006, 26(5): 1431-1437. (in Chinese)
[34]	梁兴慧. 两种蓟马的日活动规律及其对植物挥发物的趋性研究[D]. 北京: 中国农业科学院, 2010.
	LIANG X H. Study on the diurnal activity patterns of two thrip species and their tropism to plant volatiles[D]. Beijing: Chinese Academy of Agricultural Sciences, 2010. (in Chinese)
[35]	MIGIRO L N, MANIANIA N K, CHABI-OLAYE A, VANDENBERG J. Pathogenicity of entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana (Hypocreales: Clavicipitaceae) isolates to the adult pea leafminer (Diptera: Agromyzidae) and prospects of an autoinoculation device for infection in the field. Environmental Entomology, 2010, 39(2): 468-475. doi: 10.1603/EN09359
[36]	SAIN S K, KRANTHI S, KRANTHI K R, MONGA D, PAUL D, PRASAD Y G. Diversity study of Beauveria bassiana species for finding the most virulent strain to manage Bemisia tabaci in cotton. Applied Microbiology and Biotechnology, 2024, 108: 364. doi: 10.1007/s00253-024-13188-1
[37]	陈中琴, 梁文龙, 黄丽萍, 沈斌斌. 29株虫生真菌的鉴定及对烟粉虱的毒力. 华南农业大学学报, 2020, 41(4): 57-67.
	CHEN Z Q, LIANG W L, HUANG L P, SHEN B B. Identification of 29 strains of entomopathogenic fungi and their toxicity to Bemisia tabaci. Journal of South China Agricultural University, 2020, 41(4): 57-67. (in Chinese)
[38]	赵梦. 57株昆虫病原真菌致病力和虫体产孢量研究[D]. 杨凌: 西北农林科技大学, 2018.
	ZHAO M. Study of entomopathogeniticy and in vivo conidiation of 57 strains of the entomopathogenic fungi[D]. Yangling: Northwest A&F University, 2018. (in Chinese)
[39]	王海鸿, 王萌涵, 王登杰, 梁兴慧. 球孢白僵菌XJWLMQ-1及其在生物防治中的应用: CN118126847B[P]. (2024-07-23) [2026-02-13].
	WANG H H, WANG M H, WANG D J, LIANG X H. Beauveria bassiana XJWLMQ-1 and its application in biological control: CN118126847B[P]. (2024-07-23) [2026-02-13]. (in Chinese)
[40]	JOHNY S, KYEI-POKU G, GAUTHIER D, FRANKENHUYZEN K, KRELL P J. Characterization and virulence of Beauveria spp. recovered from emerald ash borer in southwestern Ontario, Canada. Journal of Invertebrate Pathology, 2012, 111(1): 41-49. doi: 10.1016/j.jip.2012.05.008
[41]	刘美竹. 九种寄主植物挥发物对橘小实蝇雌虫的诱集作用[D]. 昆明: 云南农业大学, 2023.
	LIU M Z. The attraction of nine host plant volatiles to female Bactrocera dorsalis (Hendel)[D]. Kunming: Yunnan Agricultural University, 2023. (in Chinese)
[42]	宋晓兵, 彭埃天, 崔一平, 凌金锋, 程保平, 陈霞. 九里香挥发物对柑橘木虱的引诱效果及混配筛选试验. 植物保护学报, 2019, 46(3): 589-594.
	SONG X B, PENG A T, CUI Y P, LING J F, CHENG B P, CHEN X. Trapping effects of Murraya exotica volatiles on Asian citrus psyllid Diaphorina citri and mixed screening test. Journal of Plant Protection, 2019, 46(3): 589-594. (in Chinese)
[43]	BRUCE T J A, WADHAMS L J, WOODCOCK C M. Insect host location: A volatile situation. Trends in Plant Science, 2005, 10(6): 269-274. doi: 10.1016/j.tplants.2005.04.003 pmid: 15949760
[44]	BRUCE T J A, PICKETT J A. Perception of plant volatile blends by herbivorous insects - Finding the right mix. Phytochemistry, 2011, 72(13): 1605-1611. doi: 10.1016/j.phytochem.2011.04.011
[45]	CONCHOU L, LUCAS P, MESLIN C, PROFFIT M, STAUDT M, RENOU M. Insect odorscapes: From plant volatiles to natural olfactory scenes. Frontiers in Physiology, 2019, 10: 972. doi: 10.3389/fphys.2019.00972 pmid: 31427985
[46]	WEBSTER B, CARDÉ R T. Use of habitat odour by host-seeking insects. Biological Reviews, 2017, 92(2): 1241-1249. doi: 10.1111/brv.2017.92.issue-2
[47]	HEIL M. Herbivore-induced plant volatiles: Targets, perception and unanswered questions. New Phytologist, 2014, 204(2): 297-306. doi: 10.1111/nph.2014.204.issue-2
[48]	DUDAREVA N, NEGRE F, NAGEGOWDA D A, ORLOVA I. Plant volatiles: Recent advances and future perspectives. Critical Reviews in Plant Sciences, 2006, 25(5): 417-440. doi: 10.1080/07352680600899973
[49]	李欣华, 王登杰, 雷仲仁, 王海鸿. 单头饲养和群体饲养的西花蓟马实验种群生命表比较. 中国农业科学, 2021, 54(5): 959-968. doi: 10.3864/j.issn.0578-1752.2021.05.008.
	LI X H, WANG D J, LEI Z R, WANG H H. Comparison of life tables for experimental populations of individual-rearing and group-rearing Frankliniella occidentalis. Scientia Agricultura Sinica, 2021, 54(5): 959-968. doi: 10.3864/j.issn.0578-1752.2021.05.008. (in Chinese)
[50]	BYRNE D N, BELLOWS T S. Whitefly biology. Annual Review of Entomology, 1991, 36: 431-457. doi: 10.1146/ento.1991.36.issue-1
[51]	陈景芸. 五种斑潜蝇形态特征比较研究及重要种的遗传结构分析[D]. 扬州: 扬州大学, 2019.
	CHEN J Y. Comparative study on morphological characteristics of five Liriomyza species and genetic structure analysis of important species[D]. Yangzhou: Yangzhou University, 2019. (in Chinese)
[52]	MCINNIS D O. Mediterranean fruit fly (Diptera: Tephritidae): Directional selection for large and small pupal size. Annals of the Entomological Society of America, 1987, 80(3): 333-338. doi: 10.1093/aesa/80.3.333
[53]	胡敦孝, 吴杏霞. 烟粉虱和温室白粉虱的区别. 植物保护, 2001, 27(5): 15-18.
	HU D X, WU X X. Differentiation between Bemisia tabaci and Trialeurodes vaporariorum. Plant Protection, 2001, 27(5): 15-18. (in Chinese)
[54]	SAYED S, AL-OTAIBI S, EL-SHEHAWI A, ELARNAOUTY S A, EL-SHAZLY S, GABER A, IBRAHIM R. Field evaluation of native fungus, Beauveria bassiana (Bals.) Vuillemin against some piercing-sucking insects on the grapevine. Pakistan Journal of Biological Sciences, 2021, 24: 158-164. doi: 10.3923/pjbs.2021.158.164
[55]	WRAIGHT S P, CARRUTHERS R I, JARONSKI S T, BRADLEY C A, GARZA C J, GALAINI-WRAIGHT S. Evaluation of the entomopathogenic fungi Beauveria bassiana and Paecilomyces fumosoroseus for microbial control of the silverleaf whitefly, Bemisia argentifolii. Biological Control, 2000, 17(3): 203-217. doi: 10.1006/bcon.1999.0799
[56]	CAFARCHIA C, PELLEGRINO R, ROMANO V, FRIULI M, DEMITRI C, POMBI M, BENELLI G, OTRANTO D. Delivery and effectiveness of entomopathogenic fungi for mosquito and tick control: Current knowledge and research challenges. Acta Tropica, 2022, 234: 106627. doi: 10.1016/j.actatropica.2022.106627
[57]	FRANCARDI V, BENVENUTI C, BARZANTI G P, ROVERSI P F. Autocontamination trap with entomopathogenic fungi: A possible strategy in the control of Rhynchophorus ferrugineus (Olivier) (Coleoptera Curculionidae). Journal of Zoology, 2013, 96(4): 57-67.
[58]	MANIANIA N K. A low-cost contamination device for infecting adult tsetse flies, Glossina spp., with the entomopathogenic fungus Metarhizium anisopliae in the field. Biocontrol Science & Technology, 2002, 12(1): 59-66.
[59]	雷仲仁, 吴圣勇, 王海鸿. 我国蔬菜害虫生物防治研究进展. 植物保护, 2016, 42(1): 1-6, 25.
	LEI Z R, WU S Y, WANG H H. Progresses in biological control of vegetable insect pests in China. Plant Protection, 2016, 42(1): 1-6, 25. (in Chinese)
[60]	王恩东, 吴圣勇, 吕佳乐, 姜晓环, 马兆义, 刘亚杰, 刘振州, 赵宏玉, 吴朔, 吴霞, 徐学农. 释放巴氏新小绥螨防治温室大棚番茄上的烟粉虱. 植物保护, 2020, 46(4): 234-238.
	WANG E D, WU S Y, LÜ J L, JIANG X H, MA Z Y, LIU Y J, LIU Z Z, ZHAO H Y, WU S, WU X, XU X N. Control of whitefly Bemisia tabaci on tomato plants in greenhouse by releasing Neoseiulus barkeri. Plant Protection, 2020, 46(4): 234-238. (in Chinese)
[61]	李姝, 劳水兵, 王甦, 郭晓军, 张帆. 东亚小花蝽和丽蚜小蜂对烟粉虱的协同控制效果研究. 环境昆虫学报, 2014, 36(6): 978-982.
	LI S, LAO S B, WANG S, GUO X J, ZHANG F. Control effect of Orius sauteri collaborated with Encarsia formosa on Bemisia tabaci in the greenhouse. Journal of Environmental Entomology, 2014, 36(6): 978-982. (in Chinese)
[62]	王恩东, 张博, 赵佩佩, 徐学农, 闫红, 郝学敏. 捕食螨与爪哇虫草菌联合防治粉虱的方法: CN119563485A[P]. (2025-03-07) [2026- 02-13].
	WANG E D, ZHANG B, ZHAO P P, XU X N, YAN H, HAO X M. Control of whitefly by combination of predatory mites and Cordyceps javanica: CN119563485A[P]. (2025-03-07) [2026-02-13]. (in Chinese)
[63]	SUI L, SHI N, LI Q Y, ZHAO Y, ZHANG Z K, LU Y. Improved control of Rhopalosiphum maidis and Acyrthosiphon pisum, using the predatory natural enemy Harmonia axyridis carrying entomopathogenic fungi. Pest Management Science, 2025, 81(7): 3786-3795. doi: 10.1002/ps.v81.7

菌株编号Strain ID	寄主来源Host source	采集地Collection location
LNTL-1	亚洲玉米螟Ostrinia nubilalis	辽宁铁岭Tieling, Liaoning
LNTL-3	亚洲玉米螟Ostrinia nubilalis	辽宁铁岭Tieling, Liaoning
LNTL-5	亚洲玉米螟Ostrinia nubilalis	辽宁铁岭Tieling, Liaoning
JXNC-4	褐飞虱Nilaparvata lugens	江西南昌Nanchang, Jiangxi
JXNC-5	褐飞虱Nilaparvata lugens	江西南昌Nanchang, Jiangxi
JXNC-11	褐飞虱Nilaparvata lugens	江西南昌Nanchang, Jiangxi
JXNC-12	褐飞虱Nilaparvata lugens	江西南昌Nanchang, Jiangxi
HNLY-46	温室白粉虱Trialeurodes vaporariorum	河南洛阳Luoyang, Henan
HNLY-47	温室白粉虱Trialeurodes vaporariorum	河南洛阳Luoyang, Henan
JLGZL-1	亚洲玉米螟Ostrinia nubilalis	吉林公主岭Gongzhuling, Jilin
JLGZL-2	亚洲玉米螟Ostrinia nubilalis	吉林公主岭Gongzhuling, Jilin
JLGZL-3	亚洲玉米螟Ostrinia nubilalis	吉林公主岭Gongzhuling, Jilin
JLGZL-9	亚洲玉米螟Ostrinia nubilalis	吉林公主岭Gongzhuling, Jilin
SDDZ-13	亚洲玉米螟Ostrinia nubilalis	山东德州Dezhou, Shandong
SDDZ-14	亚洲玉米螟Ostrinia nubilalis	山东德州Dezhou, Shandong
SDDZ-20	亚洲玉米螟Ostrinia nubilalis	山东德州Dezhou, Shandong
SDDZ-21	亚洲玉米螟Ostrinia nubilalis	山东德州Dezhou, Shandong
XJWLMQ-1	亚洲玉米螟Ostrinia nubilalis	新疆乌鲁木齐Urumqi, Xinjiang
XJWLMQ-33	亚洲玉米螟Ostrinia nubilalis	新疆乌鲁木齐Urumqi, Xinjiang
XJWLMQ-36	亚洲玉米螟Ostrinia nubilalis	新疆乌鲁木齐Urumqi, Xinjiang

处理 Treatment	顺-3-己烯醛﹕芳樟醇﹕丁香酚 (Z)-3-hexenal﹕Linalool﹕Eugenol	处理 Treatment	顺-3-己烯醛﹕芳樟醇﹕丁香酚 (Z)-3-hexenal﹕Linalool﹕Eugenol
B-1	1﹕1﹕1	B-6	3﹕1﹕1
B-2	1﹕2﹕1	B-7	1﹕3﹕3
B-3	1﹕1﹕3	B-8	2﹕1﹕1
B-4	2﹕2﹕1	B-9	1﹕2﹕3
B-5	1﹕1﹕2

处理 Treatment	形式 Form	食诱剂成分 Ingredient of food attractants	成分配比 Proportion of ingredients
处理1 Treatment 1	Y-1+4+5加粘虫板 Y-1+4+5 with yellow sticky trap	顺-3-己烯醛+芳樟醇+丁香酚 (Z)-3-hexenal+linalool+eugenol	1﹕2﹕1
处理2 Treatment 2	Y-1+5加粘虫板 Y-1+5 with yellow sticky trap	顺-3-己烯醛+丁香酚 (Z)-3-hexenal+eugenol	1﹕1
处理3 Treatment 3	Y-4+5加粘虫板 Y-4+5 with yellow sticky trap	芳樟醇+丁香酚 Linalool+eugenol	1﹕1
处理4 Treatment 4	Y-1+2+3+5加粘虫板 Y-1+2+3+5 with yellow sticky trap	顺-3-己烯醛+顺-3-己烯乙酸酯+对乙基苯乙酮+丁香酚 (Z)-3-hexenal+(Z)-hexenyl acetate+4-ethylacetophenone+eugenol	1﹕1﹕1﹕1
处理5 Treatment 5	Y-1+2+4加粘虫板 Y-1+2+4 with yellow sticky trap	顺-3-己烯醛+顺-3-己烯乙酸酯+芳樟醇 (Z)-3-hexenal+(Z)-hexenyl acetate+linalool	1﹕1﹕1
对照Control	粘虫板Yellow sticky trap	—	—

处理 Treatment	投放位置 Position	1 d诱集数量 Number of traps (1 d)	3 d诱集数量 Number of traps (3 d)	7 d诱集数量 Number of traps (7 d)
处理1 Treatment 1	植株中部Middle of plant	32.98±2.69b	131.28±6.74b	418.36±17.62b
	番茄顶端Top of tomato plant	42.98±2.78a	165.33±6.95a	438.50±17.51a
	番茄植株顶端20 cm处 20 cm above the top of tomato plant	25.33±3.76c	111.62±7.95c	368.45±11.75c
处理2 Treatment 2	植株中部Middle of plant	28.67±1.82b	114.33±5.12b	311.51±12.43b
	番茄顶端Top of tomato plant	36.67±2.63a	127.33±4.92a	323.18±11.21a
	番茄植株顶端20 cm处 20 cm above the top of tomato plant	23.67±1.36c	105.33±3.46c	300.14±10.90c
处理3 Treatment 3	植株中部Middle of plant	26.87±2.42b	103.21±4.81b	305.62±12.52b
	番茄顶端Top of tomato plant	32.24±2.87a	119.43±4.21a	313.24±13.25a
	番茄植株顶端20 cm处 20 cm above the top of tomato plant	18.67±2.76c	98.23±5.46c	280.61±11.90c

处理 Treatment	投放距离 Distance	1 d诱集数量 Number of traps (1 d)	3 d诱集数量 Number of traps (3 d)	7 d诱集数量 Number of traps (7 d)
处理1 Treatment 1	间隔10 m 10 m interval	54.63±2.81a	172.33±6.75a	435.00±16.62a
	间隔7 m 7 m interval	52.54±2.68a	170.35±5.68a	419.48±17.68a
	间隔4 m 4 m interval	51.56±2.16a	164.48±6.54a	421.36±17.59a
处理2 Treatment 2	间隔10 m 10 m interval	35.17±1.63a	117.23±5.25a	333.00±15.23a
	间隔7 m 7 m interval	33.67±1.16a	121.35±3.67a	335.00±15.57a
	间隔4 m 4 m interval	35.62±1.32a	114.33±4.54a	321.32±12.32a