中国农业科学 ›› 2011, Vol. 44 ›› Issue (23): 4926-4932.doi: 10.3864/j.issn.0578-1752.2011.23.020

• 兽医 • 上一篇    下一篇

鼠李糖乳酸杆菌对Caco-2细胞抗氧化功能的影响

崔志文, 黄琴, 黄怡, 吴红照, 文静, 李卫芬   

  1. 1.浙江大学动物科学学院/教育部动物分子营养学重点实验室,杭州 310029
    2.广西大学动物科学技术学院,南宁 530005
  • 收稿日期:2010-11-17 出版日期:2011-12-01 发布日期:2011-05-23
  • 通讯作者: 通信作者李卫芬,Tel:0571-86986730;E-mail:wfli@zju.edu.cn
  • 作者简介:崔志文,E-mail:czw3502002@yahoo.com.cn
  • 基金资助:

    国家重点基础研究发展计划(2009CB118705)、浙江省重大科技专项(2006C12086)

Antioxidative Function of Lacbacillus rhamnosus to Caco-2 Cells

 CUI  Zhi-Wen, HUANG  Qin, HUANG  Yi, WU  Hong-Zhao, WEN  Jing, LI  Wei-Fen   

  1. 1.浙江大学动物科学学院/教育部动物分子营养学重点实验室,杭州 310029
    2.广西大学动物科学技术学院,南宁 530005
  • Received:2010-11-17 Online:2011-12-01 Published:2011-05-23

摘要: 【目的】研究鼠李糖乳酸杆菌(Lactobacillus rhamnosus)对氧化应激状态下Caco-2细胞抗氧化功能的影响。【方法】将培养的Caco-2细胞分为4组:对照组和氧化应激组(在培养液中加入100 µmol•L-1 H2O2),处理组Ⅰ和处理组Ⅱ在氧化应激条件下分别添加鼠李糖乳酸杆菌(约108 CFU•mL-1)和抗氧化剂特丁基对苯二酚(TBHQ) (2.75 µg•mL-1)各1 mL。Caco-2细胞培养至12和48 h时分别测定其上清液和裂解液的抗氧化活性。【结果】添加鼠李糖乳酸杆菌减轻了Caco-2细胞氧化受损,使细胞培养上清中的总抗氧化力(T-AOC)显著高于氧化应激组:12 h时显著提高了细胞培养上清谷胱甘肽过氧化物酶(GSH-Px)(P<0.01)、过氧化氢酶(CAT)(P<0.01)活力以及细胞裂解液中超氧化物酶(SOD)活力(P<0.01)和还原型谷胱甘肽(GSH)含量(P<0.05),48 h时显著提高了细胞培养上清液抗O2- (ASAFR) (P<0.01)、GSH-Px(P<0.01)、CAT(P<0.01)、SOD活力(P<0.01)和细胞裂解液中过氧化物酶(POD)活力(P<0.01),丙二醛(MDA)含量显著降低(P<0.01)。添加TBHQ组12 h时细胞上清中T-AOC(P<0.01)和CAT活性(P<0.01)及细胞裂解液中GSH含量(P<0.01)显著高于氧化应激组,而处理48 h后相应指标低于氧化应激组;此时,细胞培养上清液中MDA含量极显著降低(P<0.01),抗O2-、SOD、GSH-Px、POD活力和细胞裂解液中POD活力显著升高(P<0.05)。【结论】在体外条件下,鼠李糖乳酸杆菌可以提高氧化应激状态下Caco-2细胞的抗氧化功能。

关键词: Caco-2细胞, 鼠李糖乳酸杆菌, TBHQ, 抗氧化活性, 氧化应激

Abstract: 【Objective】This experiment was conducted to study the antioxidative function of Lactobacillus rhamnosus to Caco-2 cells under oxidative stress. 【Method】 Caco-2 cells were randomly divided into 4 groups, control group and oxidative stress group (100 µmol•L-1 H2O2), treatment groupsⅠandⅡ were added with 1 mL Lactobacillus rhamnosus (about 108 CFU•mL-1) and tertiary butylhydroquinone (TBHQ) (2.75 µg•mL-1 ) under oxidative stress, respectively. Antioxidative activities in culture supernatants and lysates of Caco-2 cells at 12 h and 48 h were measured. 【Result】 Compared to oxidative stress group, the total antioxidation capacity (T-AOC), the glutathione peroxidase (GSH-Px) (P<0.01), catalase (CAT) (P<0.01) activities in the cultured supernatants, and the superoxide dismutase (SOD) activity (P<0.01), and the glutathione (GSH) content (P<0.05) in the cells lysates at 12 h in treatment groupⅠincreased, and the increases of the antisuperoxide anion radical (O2-) (P<0.01), GSH-Px (P<0.01), CAT (P<0.01), SOD (P<0.01) activities in the cells culture supernatants and the peroxidase (POD) activity (P<0.01) and the decreases of malondialdehyde (MDA) content (P<0.01) in the cells lysates at 48 h were also observed. In treatment groupⅡ, the T-AOC (P<0.01), CAT activity (P<0.01) in the cells culture supernatants and the glutathione (GSH) content (P<0.01) in the cells lysates at 12 h increased significantly, but these values and the MDA content at 48 h decreased significantly (P<0.01). The anti-superoxide anion radical (O2-), SOD, GSH-Px, POD activities in the cells culture supernatants and the POD activity in the cells lysates at 48 h increased (P<0.05).【Conclusion】These results indicated that adding Lactobacillus rhamnosus could increase the antioxidative function of Caco-2 cells under oxidative stress under in vitro conditions.

Key words: Caco-2 cells, Lactobacillus rhamnosus, TBHQ, antioxidative activities, oxidative stress