中国农业科学 ›› 2022, Vol. 55 ›› Issue (12): 2311-2323.doi: 10.3864/j.issn.0578-1752.2022.12.004
吕志伟1(),杜康1,周治国1,赵文青1,胡伟1,赵建明2,朱素芹3,王友华1()
收稿日期:
2021-09-29
接受日期:
2021-12-15
出版日期:
2022-06-16
发布日期:
2022-06-23
通讯作者:
王友华
作者简介:
吕志伟,E-mail: 基金资助:
LÜ ZhiWei1(),DU Kang1,ZHOU ZhiGuo1,ZHAO WenQing1,HU Wei1,ZHAO JianMing2,ZHU SuQin3,WANG YouHua1()
Received:
2021-09-29
Accepted:
2021-12-15
Online:
2022-06-16
Published:
2022-06-23
Contact:
YouHua WANG
摘要:
【目的】 探寻可精确指示玉米穗位叶衰老进程的优良方法及适宜性指标,明确玉米穗位叶衰老进程,为玉米精确高效栽培技术发展提供理论参考。【方法】 于2020年春、夏两季在江苏大丰(33°12′ N,120°28′ E)稻麦原种场开展田间试验,以苏玉29(SY29)为材料,基于不同施氮量(0、67.5、136.5、205.5、274.5、343.5 kg N·hm-2)构建6个叶氮浓度差异群体(N1—N6)。采用Logistic模型对吐丝后12种与玉米穗位叶衰老相关的形态及生理指标的变化动态进行拟合,对比分析不同衰老相关指标指示衰老进程的精准性。【结果】 Logistic公式y=(a-d)/(1+(x/c)b)+d对12种叶衰老相关指标变化动态的拟合趋势、拟合度R2整体优于前人所用Logistic公式y=a/(1+e-b(x-c))。基于多指标分析,春、夏玉米穗位叶衰老启动分别发生在吐丝后18.8—29.1($\bar{x}$=25.8)d、17.5—28.2($\bar{x}$=23.9)d,快速衰老发生在吐丝后30.5—37.9($\bar{x}$=34.4)d、28.0—35.7($\bar{x}$=31.8)d,衰老终止发生在吐丝后40.6—54.1($\bar{x}$=45.9)d、38.3—47.0($\bar{x}$=42.1)d。春、夏玉米由吐丝至衰老启动的25.8 d、23.9 d内有效积温分别为392.9℃和477.6℃;春、夏玉米由衰老启动至衰老终止的20.1 d、18.2 d内有效积温分别为360.0℃和284.0℃。基于指标分类分析,相同衰老阶段内,生理指标所指示的衰老特征时间的正态分布σ值大于形态指标,春、夏玉米结果一致。丙二醛(MDA)、总叶绿素、叶绿素a、果糖、葡萄糖含量及蒸腾速率(Tr)所指示衰老特征时间的变异系数(CV)均小于5%;MDA、总叶绿素、果糖、葡萄糖含量及净光合速率(Pn)所指示衰老特征时间与各阶段时间均数的均方根误差(RMSE)均小于2。【结论】 Logistic公式y=(a-d)/(1+(x/c)b)+d可作为准确拟合叶衰老指标动态变化的数学工具。春、夏玉米穗位叶分别在吐丝后26 d、24 d左右开始衰老,于34 d、32 d左右衰老速度达到峰值,于46 d、42 d左右进入功能性死亡的末端衰老阶段,有效积温是影响玉米穗位叶衰老进程重要因素。MDA、总叶绿素、果糖、葡萄糖含量是表征玉米穗位叶衰老进程的优良指标。
吕志伟,杜康,周治国,赵文青,胡伟,赵建明,朱素芹,王友华. 玉米穗位叶衰老进程及其适宜指示性指标研究[J]. 中国农业科学, 2022, 55(12): 2311-2323.
LÜ ZhiWei,DU Kang,ZHOU ZhiGuo,ZHAO WenQing,HU Wei,ZHAO JianMing,ZHU SuQin,WANG YouHua. Research on Senescence Process and Suitable Indicators of Maize Ear Leaves[J]. Scientia Agricultura Sinica, 2022, 55(12): 2311-2323.
表1
土壤基础养分含量"
年份 Year | 有机质含量 Organic matter content (g·kg-1) | 全氮含量 Total nitrogen content (g·kg-1) | 碱解氮含量 Alkali-hydrolyzable nitrogen content (mg·kg-1) | 速效磷含量 Available phosphorus content (mg·kg-1) | 速效钾含量 Available potassium content (mg·kg-1) | |
---|---|---|---|---|---|---|
2020 | 春Spring | 16.01 | 1.06 | 51.07 | 28.33 | 193.87 |
夏Summer | 13.77 | 0.93 | 50.93 | 22.74 | 199.35 |
表2
试验处理及处理效果"
试验处理 Experimental treatment | 施氮量 Nitrogen rate (kg N·hm-2) | 穗位叶氮浓度(春玉米) Nitrogen concentration of ear leaves (spring maize) (%) | 穗位叶氮浓度(夏玉米) Nitrogen concentration of ear leaves (summer maize) (%) | ||||
---|---|---|---|---|---|---|---|
20 DAS | 30 DAS | 40 DAS | 20 DAS | 30 DAS | 40 DAS | ||
N1 | 0 | 3.00d | 1.83e | 0.91f | 3.10d | 1.75d | 0.83e |
N2 | 67.5 | 3.39c | 2.54d | 1.13e | 3.11d | 1.80d | 0.95d |
N3 | 136.5 | 3.41c | 3.04c | 1.46d | 3.59c | 2.50c | 1.05c |
N4 | 205.5 | 4.21b | 3.32b | 2.02c | 3.71bc | 2.51c | 1.09c |
N5 | 274.5 | 4.82a | 4.12a | 2.73b | 3.79b | 2.84b | 1.23b |
N6 | 343.5 | 4.82a | 3.97a | 2.92a | 4.33a | 3.14a | 1.46a |
表4
不同衰老相关指标对玉米穗位叶衰老进程指示的精确性"
指示性指标 Indicative indicator | 穗位叶衰老特征时间变异系数(春玉米) CV of ear leaves senescence characteristic time (spring maize) (%) | 穗位叶衰老特征时间变异系数(夏玉米) CV of ear leaves senescence characteristic time (summer maize) (%) | ||||
---|---|---|---|---|---|---|
启动Initiation | 快速Peak | 终止Termination | 启动Initiation | 快速Peak | 终止Termination | |
比叶重 Specific leaf weight | 6.389 | 4.099 | 2.874 | 4.124 | 3.302 | 3.231 |
SPAD值 SPAD value | 6.753 | 4.148 | 4.016 | 5.102 | 3.687 | 3.348 |
总叶绿素 Total chlorophyll | 3.863 | 3.368 | 2.327 | 4.363 | 3.648 | 3.549 |
叶绿素a Chlorophyll a | 3.573 | 3.101 | 2.692 | 4.439 | 3.820 | 3.674 |
叶绿素b Chlorophyll b | 6.127 | 4.589 | 4.057 | 4.742 | 3.381 | 4.005 |
氮浓度 Nitrogen concentration | 7.806 | 5.197 | 2.321 | 7.383 | 4.103 | 1.984 |
净光合速率 Pn | 7.686 | 5.159 | 3.248 | 3.406 | 1.709 | 1.029 |
蒸腾速率 Tr | 3.893 | 3.197 | 3.262 | 3.324 | 2.908 | 2.588 |
丙二醛 Malondialdehyde | 4.895 | 2.753 | 4.319 | 4.651 | 3.151 | 1.975 |
蔗糖 Sucrose | 7.923 | 5.025 | 3.365 | 4.008 | 3.146 | 2.909 |
果糖 Fructose | 2.731 | 3.044 | 3.731 | 4.339 | 3.218 | 2.999 |
葡萄糖 Glucose | 2.275 | 1.863 | 1.721 | 0.891 | 1.241 | 1.601 |
表5
不同衰老相关指标对玉米穗位叶衰老进程指示的准确性"
指示性指标 Indicative indicator | 穗位叶衰老特征时间均方根误差(春玉米) RMSE of ear leaves senescence characteristic time (spring maize) | 穗位叶衰老特征时间均方根误差(夏玉米) RMSE of ear leaves senescence characteristic time (summer maize) | ||||
---|---|---|---|---|---|---|
启动Initiation | 快速Peak | 终止Termination | 启动Initiation | 快速Peak | 终止Termination | |
比叶重 Specific leaf weight | 2.342 | 1.623 | 2.890 | 1.506 | 0.965 | 2.365 |
SPAD值 SPAD value | 1.624 | 1.587 | 2.554 | 1.108 | 1.799 | 3.337 |
总叶绿素 Total chlorophyll | 0.946 | 1.188 | 1.530 | 1.954 | 1.321 | 1.792 |
叶绿素a Chlorophyll a | 0.917 | 1.280 | 3.913 | 2.122 | 1.259 | 2.348 |
叶绿素b Chlorophyll b | 1.719 | 2.035 | 1.965 | 2.742 | 2.194 | 1.594 |
氮浓度 Nitrogen concentration | 5.003 | 2.012 | 2.535 | 4.491 | 2.168 | 1.694 |
净光合速率 Pn | 1.897 | 1.719 | 1.811 | 0.812 | 0.716 | 0.448 |
蒸腾速率 Tr | 4.563 | 1.010 | 6.398 | 2.619 | 0.904 | 2.875 |
丙二醛 Malondialdehyde | 1.675 | 1.018 | 1.952 | 1.205 | 0.937 | 0.846 |
蔗糖 Sucrose | 2.267 | 2.085 | 2.708 | 1.129 | 0.926 | 2.246 |
果糖 Fructose | 0.707 | 0.983 | 1.567 | 0.959 | 1.142 | 1.329 |
葡萄糖 Glucose | 0.940 | 0.635 | 1.004 | 0.903 | 0.391 | 0.694 |
[1] |
FOWLER D B. Crop nitrogen demand and grain protein concentration of spring and winter wheat. Agronomy Journal, 2003, 95(2): 260-265.
doi: 10.2134/agronj2003.2600 |
[2] |
HOU P, GAO Q, XIE R Z, LI S K, MENG Q F, KIRKBY E A, RÖMHELD V, MÜLLER T, ZHANG F S, CUI Z L, CHEN X P. Grain yields in relation to N requirement: Optimizing nitrogen management for spring maize grown in China. Field Crops Research, 2012, 129: 1-6.
doi: 10.1016/j.fcr.2012.01.006 |
[3] |
KOSGEY J R, MOOT D J, FLETCHER A L, MCKENZIE B A. Dry matter accumulation and post silking N economy of stay green maize (Zea mays L.) hybrids. European Journal of Agronomy, 2013, 51: 43-52.
doi: 10.1016/j.eja.2013.07.001 |
[4] |
NOVOA R, LOOMIS R S. Nitrogen and plant production. Plant and Soil, 1981, 58(1/3): 177-204.
doi: 10.1007/BF02180053 |
[5] |
MASCLAUX C, VALADIER M H, BRUGIÈRE N, MOROT- GAUDRY J F, HIREL B. Characterization of the sink/source transition in tobacco (Nicotiana tabacum L.) shoots in relation to nitrogen management and leaf senescence. Planta, 2000, 211: 510-518.
doi: 10.1007/s004250000310 |
[6] |
ZHAO D, DERKX A P, LIU D C, BUCHNER P, HAWKESFORD M J. Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat. Plant Biology, 2015, 17: 904-913.
doi: 10.1111/plb.12296 |
[7] |
LIM P O, KIM H J, NAM H G. Leaf senescence. Annual Review of Plant Biology, 2007, 58: 115-136.
doi: 10.1146/annurev.arplant.57.032905.105316 |
[8] |
LIU T N, GU L M, DONG S T, ZHANG J W, LIU P, ZHAO B. Optimum leaf removal increases canopy apparent photosynthesis, 13C-photosynthate distribution and grain yield of maize crops grown at high density. Field Crops Research, 2015, 170: 32-39.
doi: 10.1016/j.fcr.2014.09.015 |
[9] |
陈传永, 王荣焕, 赵久然, 徐田军, 王元东, 刘秀芝, 刘春阁, 裴志超, 成广雷, 陈国平. 不同生育时期遮光对玉米籽粒灌浆特性及产量的影响. 作物学报, 2014, 40(9): 1650-1657.
doi: 10.3724/SP.J.1006.2014.01650 |
CHEN C J, WANG R H, ZHAO J R, XU T J, WANG Y D, LIU X Z, LIU C G, PEI Z C, CHENG G L, CHEN G P. Effects of shading on grain-filling properties and yield of maize at different growth stages. Acta Agronomica Sinica, 2014, 40(9): 1650-1657. (in Chinese)
doi: 10.3724/SP.J.1006.2014.01650 |
|
[10] | 王晓慧, 张磊, 刘双利, 曹玉军, 魏雯雯, 刘春光, 王永军, 边少锋, 王立春. 不同熟期春玉米品种的籽粒灌浆特性. 中国农业科学, 2014, 47(18): 3557-3565. |
WANG X H, ZHANG L, LIU S L, CAO Y J, WEI W W, LIU C G, WANG Y J, BIAN S F, WANG L C. Grain filling characteristics of maize hybrids differing in maturities. Scientia Agricultura Sinica, 2014, 47(18): 3557-3565. (in Chinese) | |
[11] |
刘开昌, 董树亭, 赵海军, 王庆成, 李宗新, 刘霞, 张慧. 我国玉米自交系叶片保绿性及其与产量的关系. 作物学报, 2009, 35(9): 1662-1671.
doi: 10.3724/SP.J.1006.2009.01662 |
LIU K C, DONG S T, ZHAO H J, WANG Q C, LI Z X, LIU X, ZHANG H. Leaf stay-green traits in Chinese maize inbred lines and their relationship with grain yield. Acta Agronomica Sinica, 2009, 35(9): 1662-1671. (in Chinese)
doi: 10.3724/SP.J.1006.2009.01662 |
|
[12] | 赵延明, 张海艳. 基于叶片面积玉米叶片保绿度开花后衰减特性初步研究. 中国农学通报, 2009, 25(12): 91-94. |
ZHAO Y M, ZHANG H Y. The primary studies on reducing characteristics of leaf stay-green based on leaf area after flowering in maize. Chinese Agricultural Science Bulletin, 2009, 25(12): 91-94. (in Chinese) | |
[13] |
BIRCH C J, HAMMER G L, RICKERT K G. Improved methods for predicting individual leaf area and leaf senescence in maize (Zea mays L.). Australian Journal of Agricultural Research, 1998, 49: 249-262.
doi: 10.1071/A97010 |
[14] |
吕国锋, 范金平, 张伯桥, 高德荣, 王慧, 刘业宇, 吴素兰, 程凯, 王秀娥. 小麦旗叶衰老过程不同数学模型拟合比较及衰老特征分析. 作物学报, 2019, 45(1): 144-152.
doi: 10.3724/SP.J.1006.2019.81014 |
LÜ G F, FAN J P, ZHANG B Q, GAO D R, WANG H, LIU Y Y, WU S L, CHENG K, WANG X E. Comparison of different mathematical models describing flag leaf senescence process of wheat and characteristics of leaf senescence process. Acta Agronomica Sinica, 2019, 45(1): 144-152. (in Chinese)
doi: 10.3724/SP.J.1006.2019.81014 |
|
[15] |
VAN OOSTEROM E J, JAYACHANDRAN R, BIDINGER F R. Diallel analysis of the stay-green trait and its components in sorghum. Crop Science, 1996, 36(3): 549-555.
doi: 10.2135/cropsci1996.0011183X003600030002x |
[16] |
KASSAHUN B, BIDINGER F R, HASH C T, KURUVINASHETTI M S. Stay-green expression in early generation sorghum [Sorghum bicolor (L.) Moench] QTL introgression lines. Euphytica, 2010, 172: 351-362.
doi: 10.1007/s10681-009-0108-0 |
[17] | MAHALAKSHMI V, BIDINGER F R. Evaluation of stay-green sorghum germplasm lines at ICRISAT. Crop Science, 2002, 42(3): 965-974. |
[18] | 薛晖, 贾丽, 龚月桦, 刘赢洲, 吴养会. 冬小麦叶片持绿能力及其衰老特征研究. 西北植物学报, 2010, 30(2): 336-343. |
XUE H, JIA L, GONG Y H, LIU Y Z, WU Y H. Study on the stay- green capacity and leaf senescence of winter wheat. Acta Botanica Boreali-Occidentalia Sinica, 2010, 30(2): 336-343. (in Chinese) | |
[19] |
LIM P O, WOO H R, NAM H. Molecular genetics of leaf senescence in arabidopsis. Trends in Plant Science, 2003, 8(6): 272-278.
doi: 10.1016/S1360-1385(03)00103-1 |
[20] |
BUCHANAN-WOLLASTON V. The molecular biology of leaf senescence. Journal of Experimental Botany, 1997, 48(2): 181-199.
doi: 10.1093/jxb/48.2.181 |
[21] |
MCMASTER G S, WILHELM W W. Is soil temperature better than air temperature for predicting winter wheat phenology? Agronomy Journal, 1998, 90(5): 602-607.
doi: 10.2134/agronj1998.00021962009000050006x |
[22] | 许东恒, 石玉海, 孙宁, 张林, 曹铁华, 王立春, 赵洪祥. 氮肥运筹对玉米叶片光合速率、比叶重和SPAD值的影响. 玉米科学, 2010, 18(6): 102-106. |
XU D H, SHI Y H, SUN N, ZHANG L, CAO T H, WANG L C, ZHAO H X. Effects of nitrogen application on photosynthetic rate, specific leaf weight and SPAD value in leaf of maize. Journal of Maize Sciences, 2010, 18(6): 102-106. (in Chinese) | |
[23] | 李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000: 186-191. |
LI H S. Principles and Techniques of Plant Physiological Biochemical Experiment. Beijing: Higher Education Press, 2000: 186-191. (in Chinese) | |
[24] | 李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000: 134-137. |
LI H S. Principles and Techniques of Plant Physiological Biochemical Experiment. Beijing: Higher Education Press, 2000: 134-137. (in Chinese) | |
[25] |
LIU J R, MA Y N, LYU F J, CHEN J, ZHOU Z G, WANG Y H, ABUDUREZIKE A, OOSTERHUIS D M. Changes of sucrose metabolism in leaf subtending to cotton boll under cool temperature due to late planting. Field Crops Research, 2013, 144: 200-211.
doi: 10.1016/j.fcr.2013.02.003 |
[26] | 刘鹏, 胡昌浩, 董树亭, 王空军, 张吉旺, 张保仁. 甜质型与普通型玉米籽粒发育过程中糖代谢相关酶活性的比较. 中国农业科学, 2005, 38(1): 52-58. |
LIU P, HU C H, DONG S T, WANG K J, ZHANG J W, ZHANG B R. Comparison of enzymes activity associated with sucrose metabolism in the developing grains between sweet corn and normal corn. Sicientia Agricultura Sinica, 2005, 38(1): 52-58. (in Chinese) | |
[27] |
HENDRIX D L. Rapid extraction and analysis of nonstuctural carbohydrates in plant tissues. Crop Science, 2010, 33(6): 1306-1311.
doi: 10.2135/cropsci1993.0011183X003300060037x |
[28] | 李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000: 260-261. |
LI H S. Principles and Techniques of Plant Physiological Biochemical Experiment. Beijing: Higher Education Press, 2000: 260-261. (in Chinese) | |
[29] |
NOODEN L D, GUIAMET J J, JOHN I. Senescence mechanisms. Physiologia Plantarum, 2010, 101(4): 746-753.
doi: 10.1111/j.1399-3054.1997.tb01059.x |
[30] | 薛生梁, 刘明春, 张惠玲. 河西走廊玉米生态气候分析与适生种植气候区划. 中国农业气象, 2003, 24(2): 12-15. |
XUE S L, LIU M C, ZHANG H L. Analysis and division of ecological and climatic conditions for maize in the Hexi Corridor. Chinese Journal of Agrometeorology, 2003, 24(2): 12-15. (in Chinese) | |
[31] | 李向岭, 李从锋, 侯玉虹, 侯海鹏, 葛均筑, 赵明. 不同播期夏玉米产量性能动态指标及其生态效应. 中国农业科学, 2012, 45(6): 1074-1083. |
LI X L, LI C F, HOU Y H, HOU H P, GE J Z, ZHAO M. Dynamic characteristics of summer maize yield performance in different planting dates and its effect of ecological factors. Scientia Agricultura Sinica, 2012, 45(6): 1074-1083. (in Chinese) | |
[32] |
NING P, FRITSCHI F B, LI C J. Temporal dynamics of post-silking nitrogen fluxes and their effects on grain yield in maize under low to high nitrogen inputs. Field Crops Research, 2017, 204: 249-259.
doi: 10.1016/j.fcr.2017.01.022 |
[33] | 刘春晓, 赵海军, 董树亭, 王庆成, 李宗新, 刘开昌. 玉米不同基因型双列杂交后代抽丝后氮素代谢特性. 中国农业科学, 2014, 47(1): 33-42. |
LIU C X, ZHAO H J, DONG S T, WANG Q C, LI Z X, LIU K C. Study on characteristics of nitrogen metabolism in diallel cross generation of different maize genotypes after silking. Scientia Agricultura Sinica, 2014, 47(1): 33-42. (in Chinese) | |
[34] |
ZHANG Z S, LI G, GAO H Y, ZHANG L T, YANG C, LIU P, MENG Q W. Characterization of photosynthetic performance during senescence in stay-green and quick-leaf-senescence Zea mays L. inbred lines. Plos One, 2012, 7(8): e42936.
doi: 10.1371/journal.pone.0042936 |
[35] | WU L C, LI M N, TIAN L, WANG S X, WU L J, KU L X, ZHNAG J, SONG X H, LIU H P, CHEN Y H. Global transcriptome analysis of the maize (Zea mays L.) inbred line 08LF during leaf senescence initiated by pollination-prevention. Plos One, 2017, 12(10): e185838. |
[36] |
THOMAS H, OUGHAM H. The stay-green trait. Journal of Experimental Botany, 2014, 65(14): 3889-3900.
doi: 10.1093/jxb/eru037 |
[37] | MAE T, THOMAS H, GAY A P, MAKINO A, HIDEMA J. Leaf development in lolium temulentum: photosynthesis and photosynthetic proteins in leaves senescing under different irradiances. Plant and Cell Physiology, 1993, 34(3): 391-399. |
[38] |
SMART C M, HOSKEN S E, THOMAS H, GREAVES J A, BLAIR B G, SCHUCH W. The timing of maize leaf senescence and characterisation of senescence-related cDNAs. Physiologia Plantarum, 1995, 93(4): 673-682.
doi: 10.1111/j.1399-3054.1995.tb05116.x |
[1] | 柴海燕,贾娇,白雪,孟玲敏,张伟,金嵘,吴宏斌,苏前富. 吉林省玉米穗腐病致病镰孢菌的鉴定与部分菌株对杀菌剂的敏感性[J]. 中国农业科学, 2023, 56(1): 64-78. |
[2] | 赵政鑫,王晓云,田雅洁,王锐,彭青,蔡焕杰. 未来气候条件下秸秆还田和氮肥种类对夏玉米产量及土壤氨挥发的影响[J]. 中国农业科学, 2023, 56(1): 104-117. |
[3] | 李周帅,董远,李婷,冯志前,段迎新,杨明羡,徐淑兔,张兴华,薛吉全. 基于杂交种群体的玉米产量及其配合力的全基因组关联分析[J]. 中国农业科学, 2022, 55(9): 1695-1709. |
[4] | 熊伟仡,徐开未,刘明鹏,肖华,裴丽珍,彭丹丹,陈远学. 不同氮用量对四川春玉米光合特性、氮利用效率及产量的影响[J]. 中国农业科学, 2022, 55(9): 1735-1748. |
[5] | 李易玲,彭西红,陈平,杜青,任俊波,杨雪丽,雷鹿,雍太文,杨文钰. 减量施氮对套作玉米大豆叶片持绿、光合特性和系统产量的影响[J]. 中国农业科学, 2022, 55(9): 1749-1762. |
[6] | 马小艳,杨瑜,黄冬琳,王朝辉,高亚军,李永刚,吕辉. 小麦化肥减施与不同轮作方式的周年养分平衡及经济效益分析[J]. 中国农业科学, 2022, 55(8): 1589-1603. |
[7] | 李前,秦裕波,尹彩侠,孔丽丽,王蒙,侯云鹏,孙博,赵胤凯,徐晨,刘志全. 滴灌施肥模式对玉米产量、养分吸收及经济效益的影响[J]. 中国农业科学, 2022, 55(8): 1604-1616. |
[8] | 张家桦,杨恒山,张玉芹,李从锋,张瑞富,邰继承,周阳晨. 不同滴灌模式对东北春播玉米籽粒淀粉积累及淀粉相关酶活性的影响[J]. 中国农业科学, 2022, 55(7): 1332-1345. |
[9] | 谭先明,张佳伟,王仲林,谌俊旭,杨峰,杨文钰. 基于PLS的不同水氮条件下带状套作玉米产量预测[J]. 中国农业科学, 2022, 55(6): 1127-1138. |
[10] | 冯宣军, 潘立腾, 熊浩, 汪青军, 李静威, 张雪梅, 胡尔良, 林海建, 郑洪建, 卢艳丽. 南方地区120份甜、糯玉米自交系重要目标性状和育种潜力分析[J]. 中国农业科学, 2022, 55(5): 856-873. |
[11] | 刘苗,刘朋召,师祖姣,王小利,王瑞,李军. 氮磷配施下夏玉米临界氮浓度稀释曲线的构建与氮营养诊断[J]. 中国农业科学, 2022, 55(5): 932-947. |
[12] | 乔远,杨欢,雒金麟,汪思娴,梁蓝月,陈新平,张务帅. 西北地区玉米生产投入及生态环境风险评价[J]. 中国农业科学, 2022, 55(5): 962-976. |
[13] | 黄兆福, 李璐璐, 侯梁宇, 高尚, 明博, 谢瑞芝, 侯鹏, 王克如, 薛军, 李少昆. 不同种植区玉米生理成熟后田间站秆脱水的积温需求[J]. 中国农业科学, 2022, 55(4): 680-691. |
[14] | 石习, 宁丽华, 葛敏, 邬奇, 赵涵. 玉米氮状况相关生物标记物的筛选和应用[J]. 中国农业科学, 2022, 55(3): 438-450. |
[15] | 张建军, 党翼, 赵刚, 王磊, 樊廷录, 李尚中. 覆膜时期和施氮量对陇东旱塬玉米产量和水氮利用效率的影响[J]. 中国农业科学, 2022, 55(3): 479-490. |
|