中国农业科学 ›› 2018, Vol. 51 ›› Issue (15): 2980-2989.doi: 10.3864/j.issn.0578-1752.2018.15.013

• 食品科学与工程 • 上一篇    下一篇

采后O3处理对采前CPPU处理猕猴桃果实乙烯代谢的影响

苏苗1,罗安伟1,李琳1,李圆圆1,白俊青1,李锐1,方沂蒙1,宋俊奇1,蔺志颖1,刘占德2

 
  

  1. 1西北农林科技大学食品科学与工程学院,陕西杨凌 712100;2西北农林科技大学园艺学院,陕西杨凌 712100
  • 收稿日期:2018-03-09 出版日期:2018-08-01 发布日期:2018-08-01
  • 通讯作者: 罗安伟,E-mail:luoanwei@nwsuaf.edu.cn
  • 作者简介:苏苗,E-mail:745396849@qq.com
  • 基金资助:
    陕西省农业科技创新与攻关项目(2015NY051)、陕西省重点研发计划农业领域重点项目(2018ZDXM-NY-056)、杨凌示范区产学研用协同创新重大项目(2018CXY-04)

Effect of Postharvest O3 Treatment on Ethylene Metabolism of Kiwifruit Preharvest Treated with CPPU

SU Miao1, LUO Anwei1, LI Lin1, LI Yuanyuan1, BAI Junqing1, LI Rui1, FANG Yimeng1, SONG Junqi1, LIN Zhiying1, Liu Zhande2   

  1. 1College of food science and Engineering Northwest A&F University, Yangling 712100, Shaanxi; 2College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi
  • Received:2018-03-09 Online:2018-08-01 Published:2018-08-01

摘要: 【目的】探究臭氧(ozone,O3)是否能有效减轻膨大剂(N-2-氯-4-吡啶基苯-N’-苯基脲,CPPU)对猕猴桃带来的负面影响,为市场上使用CPPU猕猴桃的贮藏提供信息。【方法】以采前盛花期28d使用20 mg·L-1 CPPU处理和对照用清水蘸果处理的秦美猕猴桃为试验材料,研究不同浓度的O3处理(0、10、40、70 mg·m-3)对贮藏期间猕猴桃果实乙烯代谢过程中的蛋氨酸(methionine,Met)、S-腺苷蛋氨酸(S-adenosyl methionine,SAM)、1-氨基环丙烷-1-羧酸(1-aminocyclopropane-1-carboxylic acid,ACC)含量及其相关代谢酶1-氨基环丙烷-1-羧酸合成酶(ACC synthase,ACS)和1-氨基环丙烷-1-羧酸氧化酶(ACC oxidase,ACO)活性的影响。【结果】CK组(未使用CPPU也未使用O3处理的为CPPU对照组CK)在贮藏过程中Met、SAM含量以及SAM合成酶、ACS、ACO活性的下降速率均低于CK1组(使用CPPU但未使用O3处理的为臭氧处理对照组CK1);贮藏60 d时,CK1和各O3处理(10、40、70 mg·m-3)的Met含量分别为1.36、2.62、4.41和2.60 mg·(100 g)-1,O3处理显著高于CK1(P<0.05);CK1和40 mg·m-3 O3处理的SAM含量分别为15.48 mg·(100 g)-1和20.73 mg·(100 g)-1,具有显著性差异(P<0.05),而CK1和10 mg·m-3 O3处理组、70 mg·m-3 O3处理组无显著性差异(P>0.05);CK1和各O3处理(10、40、70 mg·m-3)的ACC含量分别为0.068、0.059、0.038和0.055 nmol·g-1,40、70 mg·m-3 O3处理与CK1具有显著性差异(P<0.05);CK1和各O3处理(10、40、70 mg·m-3)的ACS活性分别为0.084、0.069、0.054和0.080 nmol·(g·h)-1;ACO活性的峰值分别为0.062、0.046、0.029和0.051 nmol·(g·h)-1,O3处理和CK1之间存在显著性差异(P<0.05);CK1和各O3处理(10、40、70 mg·m-3)的乙烯的峰值分别为18.42、15.99、9.86、11.69 μL·kg-1·h-1;呼吸高峰分别是18.77、16.15、12.24、15.48 mg·kg-1·h-1。【结论】CPPU增加了猕猴桃乙烯释放量,加速了果实软化,对猕猴桃贮藏带来负面影响,O3处理能有效抑制猕猴桃的乙烯代谢,延缓果实软化,因此O3处理能有效减缓因使用CPPU而导致的猕猴桃后熟软化进程。

关键词: 臭氧, 膨大剂, 猕猴桃, 乙烯代谢, 呼吸速率

Abstract: 【Objective】 The objective of this study was to investigate whether or not the ozone (ozone, O3) could effectively reduce the negative effects of the swelling agent (N-2-chloro-4-pyridine benzene-N'- phenyl urea, CPPU) on kiwifruit, which would provide useful information for using of CPPU on the market for the storage.【Method】First, the Qinmei kiwifruit was treated with 20 mg·L-1 CPPU in the growing season, then treated with 10, 40, 70 mg·m-3 ozone respectively in postharvest. The material contents in kiwifruit of methionine (methionine, Met), S-adenosyl methionine (S-adenosyl methionine, SAM), 1-aminocyclopropane-1- carboxylic acid (1-aminocyclopropane-1-carboxylic acid, ACC) and the related metabolic enzyme activity of ACC synthase (ACC synthase, ACS) and ACC oxidase (ACC oxidase, ACO) were studied. 【Result】The decreasing rate of Met, SAM and activity of SAM synthase, ACS, ACO under CK (without the use of CPPU and O3 treatment as CPPU control CK group) during storage was lower than that under CK1 (using CPPU without using O3 treatment for ozone treatment control CK1 group). At 60 days after kiwifruit storage, the contents of Met under CK1 and each treatment group (10, 40 and 70 mg·m-3 ozone) were 1.36, 2.62, 4.41 and 2.60 mg·(100 g)-1, respectively. The contents under O3 treatment was significantly higher than that under CK1 (P<0.05). The contents of SAM under CK1 and 40 mg·m-3 O3 group were 15.48 mg·(100 g)-1 and 20.73 mg·(100 g)-1, respectively (P<0.05), but the contents under CK1 10 mg·m-3 O3 treatment, and 70 mg·m-3 O3 treatment no significant difference (P>0.05). The ACC contents under CK1 and each treatment group (10, 40 and 70 mg·m-3 ozone) were 0.068, 0.059, 0.038 and 0.055 nmol·g-1, respectively. There were significant differences (P<0.05) between 40, 70 mg·m-3 O3 treatments and CK1. The ACS activities in CK1 group and each treatment group (10, 40and 70 mg·m-3) were 0.084, 0.069, 0.054 and 0.080 nmol·(g·h)-1, respectively. The peak of ACO activity was 0.062, 0.046, 0.029 and 0.051 nmol·(g·h)-1, respectively. There was significant difference between the treatment and the CK1 (P<0.05). The peak values of ethylene in CK1 and each treatment (10, 40, and 70 mg·m-3 ozone) were 18.42, 15.99, 9.86 and 11.69 μL·kg-1·h-1, respectively, and the peak of respiration was 18.77, 16.15, 12.24 and 15.48 mg·kg-1·h-1, respectively.【Conclusion】CPPU increased the kiwifruit ethylene production and accelerated fruit softening, had a negative impact on kiwifruit storage, while O3 could effectively inhibit the ethylene metabolism of kiwifruit. O3 treatment could effectively slow down the kiwifruit ripening due to the use of CPPU.

Key words: ozone, CPPU, kiwifruit, ethylene metabolism, respiration rate