白菜型冬油菜抗寒相关性状的遗传分析

doi:10.3864/j.issn.0578-1752.2016.21.003

中国农业科学 ›› 2016, Vol. 49 ›› Issue (21): 4085-4095.doi: 10.3864/j.issn.0578-1752.2016.21.003

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

白菜型冬油菜抗寒相关性状的遗传分析

刘林波¹，孙万仓¹，刘自刚¹，武军艳¹，方彦¹，李学才¹，曾秀存²，杨刚¹，董云³，陈奇¹，方园¹，袁金海¹

¹甘肃农业大学农学院/甘肃省油菜工程技术研究中心/甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室，兰州 730070
²河西学院，甘肃张掖734000
³甘肃省农业科学院，兰州 730070

收稿日期:2016-06-27 出版日期:2016-11-01 发布日期:2016-11-01
通讯作者: 孙万仓，E-mail：18293121851@163.com
作者简介:刘林波，E-mail：444560733@qq.com
基金资助:
国家自然科学基金（31460356, 31260334）、国家重点基础研究发展计划（973计划）（2011CB109300）、国家高技术研究发展计划（863计划）（2011AA10A104）、国家现代农业产业技术体系建设专项（CARS-13）、国家农业科技成果转化项目（2014G10000317）、甘肃省自然科学基金（145RJZG050）、油菜杂种优势利用技术与强优势杂交种创制（2016YFD0101300）

Genetic Analysis of Traits Related to Cold Resistance in Winter Rapeseed (Brassica rapa L.)

LIU Lin-bo¹, SUN Wan-cang¹, LIU Zi-gang¹, WU Jun-yan¹, FANG Yan¹, LI Xue-cai¹, ZENG Xiu-cun², YANG Gang¹, DONG yun³, CHEN Qi¹, FANG Yuan¹, YUAN Jin-hai¹

¹Agronomy College, Gansu Agricultural University/Rapeseed Engineering Research Center of Gansu Province/Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province , Lanzhou 730070
² Hexi University, Zhangye 734000, Gansu
³ Gansu Academy of Agricultural Sciences, Lanzhou 730070

Received:2016-06-27 Online:2016-11-01 Published:2016-11-01

摘要/Abstract

摘要： 【目的】了解白菜型冬油菜抗寒相关性状的遗传方式，为油菜抗寒性的遗传育种提供一定的理论依据。【方法】以6个抗寒性不同的白菜型冬油菜品种(系)为亲本材料，按照GriffingⅠ配制完全双列杂交组合，将亲本和F1种植后测定抗寒性相关的性状以获得试验数据。采用QGAStation软件中的加性-显性遗传模型和MINQUE（1）法，对白菜型冬油菜越冬率及11个理化性状的遗传方差分量比率、遗传相关性、亲本加性效应、组合显性效应和遗传率以及杂种优势进行分析。【结果】越冬率、POD活性、SOD活性、可溶性糖含量、相对电导率、叶绿素含量、蒸腾速率和气孔导度的遗传主要以加性效应为主，显性效应影响较小；CAT活性、丙二醛含量、可溶性蛋白含量和净光合速率则主要以显性效应为主。在基因型和表现型的相关分析上，越冬率与CAT活性、POD活性、SOD活性、可溶性蛋白含量、可溶性糖含量、叶绿素含量、蒸腾速率、气孔导度和净光合速率呈极显著正相关，与丙二醛含量和相对电导率则呈极显著负相关。陇油7号和陇油8号在越冬率、CAT活性、POD活性、SOD活性、可溶性蛋白含量、可溶性糖含量、叶绿素含量、蒸腾速率、气孔导度和净光合速率上的加性效应均呈正值，表明陇油7号和陇油8号可用作提高后代抗寒性的亲本。显性效应分析表明，陇油7号×GY和陇油7号×陇油8号是抗寒性强的组合。狭义遗传率以SOD活性、相对电导率、蒸腾速率和越冬率的表现最好，分别为74.63%、59.06%、57.49%和56.87%，CAT活性最低，为2.83%。CAT活性、可溶性蛋白含量和净光合速率3个抗寒相关性状具有很强的群体平均优势和群体超亲优势。【结论】越冬率、SOD活性、相对电导率和蒸腾速率适宜在早代选择，亲本评价分析指出，陇油7号的一般配合力高, 可作为冬油菜抗寒性改良的首选亲本。

关键词: 冬油菜, 加性效应, 显性效应, 抗寒性, 杂种优势

Abstract: 【Objective】The objective of this paper is to understand genetic way and to provide a theoretical foundation of cold resistance traits in winter rapeseed (Brassica rapa L.). 【Method】 In this study, six winter rapeseed cultivars with different cold resistances were used as parent materials to make complete diallel cross combinations with the Griffing methodⅠ. The parents and F1 generations were planted to obtain the data of cold resistance traits. By using the additive-dominant model and MINQUE(1) method, the genetic variance, genetic correlation, parent additive effects, combination dominant effects and heritability, and heterosis of over wintering rate and 11 traits in winter rapeseed were analyzed.【Result】Over wintering rate, POD activity, SOD activity, SS content, relative electrolytic leakage, chlorophyll content, transpiration rate and stomatal conductance were mainly controlled by additive effects, and dominant effects were much less important. The dominant effects prevailed in the traits of CAT activity, MDA content, SP content and net photosynthetic rate. Over wintering rate was detected to be significantly and positively correlated with CAT activity, POD activity, SOD activity, SP content, SS content, chlorophyll content, transpiration rate, stomatal conductance and net photosynthetic rate, but negatively correlated with MDA content and relative electrolytic leakage. Longyou 7 and Longyou 8 had positively additive effects in the traits of overwintering rate, CAT activity, POD activity, SOD activity, SP content, SS content, chlorophyll content, transpiration rate, stomatal conductance and net photosynthetic rate, which indicated Longyou 7 and Longyou 8 could be used as parents that improved cold resistance of generations. The results of dominant effects analysis showed that Longyou 7×GY and Longyou 7×Longyou 8 had stronger cold resistance. As for the narrow heritabilities, SOD activity, relative electrolytic leakage, transpiration rate and overwintering rate were 74.63%, 59.06%, 57.49% and 56.87%, respectively, and CAT activity was the lowest (2.83%). CAT activity, SP content and net photosynthetic rate had stronger population mean heterosis and population over-parent heterosis. 【Conclusion】Overwintering rate, SOD activity, relative electrolytic leakage and transpiration rate were used in selection at early generation. Among the 6 elite breeding lines, Longyou 7 was recommended for using as direct parental line due to its higher general combining ability in cold resistance traits.

Key words: winter rapeseed, additive effect, dominant effect, cold resistance, heterosis

刘林波，孙万仓，刘自刚，武军艳，方彦，李学才，曾秀存，杨刚，董云，陈奇，方园，袁金海. 白菜型冬油菜抗寒相关性状的遗传分析[J]. 中国农业科学, 2016, 49(21): 4085-4095.

LIU Lin-bo, SUN Wan-cang, LIU Zi-gang, WU Jun-yan, FANG Yan, LI Xue-cai, ZENG Xiu-cun, YANG Gang, DONG yun, CHEN Qi, FANG Yuan, YUAN Jin-hai. Genetic Analysis of Traits Related to Cold Resistance in Winter Rapeseed (Brassica rapa L.)[J]. Scientia Agricultura Sinica, 2016, 49(21): 4085-4095.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2016.21.003

https://www.chinaagrisci.com/CN/Y2016/V49/I21/4085

参考文献

[1] 刘后利. 几种芸薹属油菜的起源和进化. 作物学报, 1984, 10(1): 9-18.

Liu H L. Origin and evolution of rapeseeds. Acta Agronomica Sinica, 1984, 10(1): 9-18. (in Chinese)

[2] 孙万仓, 马卫国, 雷建民, 刘秦, 杨仁义, 武军艳, 王学芳, 叶剑, 曾军, 张亚宏, 康艳丽, 郭秀娟, 魏文惠, 杨杰, 蒲媛媛, 曾潮武, 刘红霞. 冬油菜在西北旱寒区的适应性和北移的可行性研究. 中国农业科学, 2007, 40(12): 2716-2726.

Sun W C, Ma W G, Lei J M, Liu Q, Yang R Y, Wu J Y, Wang X F, Ye J, Zeng J, Zhang Y H, Kang Y L, Guo X J, Wei W H, Yang J, Pu Y Y, Zeng C W, Liu H X. Study on adaptation and introduction possibility of winter rapeseed to dry and cold areas in north-west china. Scientia Agricultura Sinica, 2007, 40(12): 2716-2726. (in Chinese)

[3] 孙万仓, 曾秀存, 刘自刚, 杨刚, 方彦, 武军艳, 李学才. 轮回选择对白菜型冬油菜抗寒性及经济性状的影响. 中国油料作物学报, 2015, 37(4): 443-452.

Sun W C, Zeng X C, Liu Z G, Yang G, Fang Y, Wu J Y, Li X C. Effects of recurrent selection on cold tolerance and economic traits of Brassica rapa. Chinese Journal of Oil Crop Sciences,2015, 37(4): 443-452. (in Chinese)

[4] 沈金雄, 傅廷栋, 杨光圣, 马朝芝, 涂金星. 甘蓝型油菜杂种优势及产量性状的遗传改良. 中国油料作物学报, 2005, 27(1): 5-9.

Shen J X, Fu T D, Yang G S, Ma C Z, Tu J X. Analysis of heterosis reveals genetic improvement for yield traits in rapessed (Brassica napus L.). Chinese Journal of Oil Crop Sciences, 2005, 27(1): 5-9. (in Chinese)

[5] 邓武明, 阳小虎, 文凤君, 陈胜荣, 赵昌斌. 甘蓝型油菜产量性状的遗传及相关与通径分析. 中国油料作物学报, 2004, 25(4): 27-30.

Deng W M, Yang X H, Wen F J, Chen S R, Zhao C B. Inheritance model, correlation and path analysis of yield in rape (Brassica napus L.). Chinese Journal of Oil Crop Sciences,2004, 25(4): 27-30. (in Chinese)

[6] Ali M, Copeland L O, Elias S G, Kelly J D. Relationship between genetic distance and heterosis for yield and morphological traits in winter canola (Brassica napus L.). Theoretical and Applied Genetics, 1995, 91(1): 118-121.

[7] Pradhan A K, Sodhi Y S, Mukhopadhyay A, Pental D. Heterosis breeding in Indian mustard (Brassica juncea L.Czern & Coss): analysis of component characters contributing to heterosis for yield. Euphytica, 1993, 69(3): 219-229.

[8] Jiang J, Pu X, Zhang J, LI H, CHAI L, HUANG C, HU H, ZHENG B, NIU Y, JIANG L. Analysis on combining ability and heritability of the main agronomic characters for JA CMS in Brassica napus L.. Agricultural Science & Technology, 2014, 15(1): 39.

[9] 魏忠芬, 李德文, 王军, 张太平. 甘蓝型杂交油菜亲本含油量及主要农艺性状配合力与遗传力分析. 西北农业学报, 2014, 23(6): 102-108.

Wei Z F, Li D W, Wang J, Zhang T P. Analysis on combining ability and heritability of main agronomic traits and oil contents of hybrid parents in Brassica napus L.. Acta Agriculturae Boreali- occidentalis Sinica, 2014, 23(6): 102-108. (in Chinese)

[10] 姜磊, 陶诗顺, 张敏, 黄霞, 彭雅利. 甘蓝型油菜亲本主要农艺性状的配合力与遗传力分析. 西北农业学报, 2011, 3(2): 5.

Jiang L, Tao S S, Zhang M, Huang X, Peng Y L. Analysis on combining ability and genetic ability of main agronomic characters in Brassica Napus L.. Acta Agriculturae Boreali-occidentalis Sinica, 2011, 3(2): 5. (in Chinese)

[11] 刘定富, 刘后利. 甘蓝型油菜脂肪酸成分的基因作用形式和效应. 作物学报, 1990, 16(3): 193-199.

Liu D F, Liu H L. Gene action and effects of fatty acids in Brassica napus L.. Acta Agronomica Sinica, 1990, 16(3): 193-199. (in Chinese)

[12] 戚存扣, 盖钧镒, 章元明. 甘蓝型油菜芥酸含量的主基因+多基因遗传. 遗传学报, 2001, 28(2): 182-187.

Qi C K, Gai J Y, Zhang Y M. Major gene plus poly-gene inheritance of erucic acid content in Brassica napus L.. Acta Genetic Sinica, 2001, 28(2): 182-187. (in Chinese)

[13] Burns M J, Barnes S R, Bowman J G, Clarke M H E, Werner C P, Kearsey M J. QTL analysis of an intervarietal set of substitution lines in Brassica napus(i) Seed oil content and fatty acid composition. Heredity, 2003, 90(1): 39-48.

[14] 杨宁宁, 孙万仓, 刘自刚, 史鹏辉, 方彦, 武军艳, 曾秀存, 孔德晶, 鲁美宏, 王月. 北方冬油菜抗寒性的形态与生理机制. 中国农业科学, 2013, 47(3): 452-461.

Yang N N, Sun W C, Liu Z G, Shi P H, Fang Y, Wu J Y, Zeng X C, Kong D J, Lu M H, Wang Y. Morphological characters and physiological mechanisms of cold resistance of winter rapeseed in Northern China. Scientia Agricultura Sinica, 2013, 47(3): 452-461. (in Chinese)

[15] 刘自刚, 孙万仓, 杨宁宁, 王月, 何丽, 赵彩霞, 史鹏飞, 杨刚, 李学才, 武军艳, 方彦, 曾秀存. 冬前低温胁迫下白菜型冬油菜抗寒性的形态及生理特征. 中国农业科学, 2013, 46(22): 4679-4687.

Liu Z G, Sun W C, Yang N N, Wang Y, He L, Zhao C X, Shi P F, Yang G, Li X C, Wu J Y, Fang Y, Zeng X C. Morphology and physiological characteristics of cultivars with different levels of cold-resistance in winter rapeseed (Brassica campestris L.) during cold acclimation. Scientia Agricultura Sinica, 2013, 46(22): 4679-4687. (in Chinese)

[16] 史鹏辉, 孙万仓, 赵彩霞. 低温下抗氧化酶活性与冬油菜根细胞结冰关系的初步研究. 西北植物学报, 2013, 33(2): 329-335.

Shi P H, Sun W C, Zhao C X. Preliminary study on the relation of antioxidant enzyme activities at low temperature and the ice formation in root cells of winter rapeseed. Acta Botanica Boreali-occidentalis Sinica, 2013, 33(2): 329-335. (in Chinese)

[17] 刘自刚, 孙万仓, 方彦, 李学才, 杨宁宁, 武军艳, 曾秀存, 王月. 夜间低温对白菜型冬油菜光合机构的影响. 中国农业科学, 2015, 48(4): 672-682.

Liu Z G, Sun W C, Fang Y, Li X C, Yang N N, Wu J Y, Zeng X C, Wang Y. Effects of low nocturnal temperature on photosynthetic apparatus of winter rapeseed (Brassica campestris L.). Scientia Agricultura Sinica, 2015, 48(4): 672-682. (in Chinese)

[18] 孔德晶, 王月, 孙万仓, 曾秀存, 方彦. 鲁美宏, 杨宁宁. 北方白菜型冬油菜F₂主要生理生化特性的变异与抗寒性相关分析. 草业学报, 2014, 23(4): 79-86.

Kong D J, Wang Y, Sun W C, Zeng X C, Fang Y, Lu M H, Yang N N. Analysis of variation in physio-biochemical characteristics and cold resistance in winter rapeseed F₂ populations. Acta Prataculturae Sinica, 2014, 23(4): 79-86. (in Chinese)

[19] 朱惠霞, 孙万仓, 邓斌, 燕妮, 武军艳, 范惠玲, 叶剑, 曾军, 刘雅丽, 张亚宏. 白菜型冬油菜品种的抗寒性及其生理生化特性. 西北农业学报, 2007, 16(4): 34-38.

Zhu H X, Sun W C, Deng B, Yan N, Wu J Y, Fan H L, Ye J, Zeng J, Liu Y L, Zhang Y H. Study on cold hardiness and its physiological and biochemical characteristics of winter turnip rape (Brassica campetris). Acta Agriculturae Boreali-occidentalis Sinica, 2007, 16(4): 34-38. (in Chinese)

[20] 蒲媛媛, 孙万仓. 白菜型冬油菜抗寒性与生理生化特性关系. 分子植物育种, 2010, 8(2): 335-339.

Pu Y Y, Sun W C. The Relationship between cold resistance of winter turnip rape varieties and its physiological characteristics. Molecular Plant Breeding, 2010, 8(2): 335-339. (in Chinese)

[21] 常硕其, 邓启云, 罗祎, 陈小龙. 超级杂交稻及其亲本的耐冷性研究. 杂交水稻, 2015, 30(1): 51-57.

Chang S Q, Deng Q Y, Luo Y, Chen X L. Studies on cold tolerance of super hybrid rice and its parents. Hybrid Rice, 2015, 30(1): 51-57. (in Chinese)

[22] 贺荣华, 左佳, 李丁, 谢灵灵, 韩小霞, 高婧, 舒服, 李祺, 曹孟良. 水稻苗期抗寒性的杂种优势分析. 生命科学研究, 2011, 15(4): 356-358.

He R H, Zuo J, Li D, Xie L L, Han X X, Gao J, Shu F, Li Q, Cao M L. Analysis on the heterosis of cold tolerance at seedling stage in rice. Life Scencei Research, 2011, 15(4): 356-358. (in Chinese)

[23] 裴玉贺, 王小丽, 张恩盈, 宋希云. 玉米抗寒生理指标的遗传效应分析. 植物生理学报, 2011, 47(3): 293-297.

Pei Y H, Wang X L, Zhang E Y, Song X Y. Genetic analysis of physiological indices related to cold resistance in maize. Plant Physiology Journal, 2011, 47(3): 293-297. (in Chinese)

[24] 朱军. 数量性状的一般遗传模型和新的分析方法. 浙江农业大学学报, 1994, 20: 551-559.

Zhu J. General genetic models and new analysis methods for quantitative traits. Journal of Zhejiang Agricultura Univercity, 1994, 20: 551-559. (in Chinese)

[25] 邹奇. 植物生理学实验指导. 北京: 中国农业出版社, 2000.

Zou Q. Instruction in Plant Physiological Experiment. Beijing: China Agriculture Press, 2000. (in Chinese)

[26] 朱军. 遗传模型分析方法. 中国农业出版社, 1997.

Zhu J. New Analysis Methods for Genetic Models. Beijing: China Agriculture Press, 1997. (in Chinese)

[27] 朱军, 季道藩, 许馥华. 作物品种间杂种优势遗传分析的新方法. 遗传学报, 1993, 20(3): 262-271.

Zhu J, Ji D F, Xu F H. A genetic approach for analyzing intra-cultivar heterosis in crops. Acta Genetic Sinica, 1993, 20(3): 262-271. (in Chinese)

[28] 郭海林, 高雅丹, 薛丹丹, 陈宣, 刘建秀. 结缕草属植物抗寒性的遗传分析. 草业学报, 2009, 18(3): 53.

Guo H L, Gao Y D, Xue D D, Chen X, Liu J X. Genetic analysis of cold tolerance of zoysia grass. Acta Prataculturae Sinica, 2009, 18(3): 53. (in Chinese)

[29] 王鹏良, 徐洋, 吕智鹏, 王海燕, 覃子海, 刘建秀, 王秀娥. 假俭草杂种F₁抗寒性遗传分析. 草业学报, 2011, 20(2): 290-294.

Wang P L, Xu Y, Lü Z P, Wang H Y, Qin Z H, Liu J X, Wang X E. Genetic analysis for cold tolerance of F₁ hybrids in Eremochloa ophiuroides. Acta Prataculturae Sinica, 2011, 20(2): 290-294. (in Chinese)

[30] 张扬勇, 靳哲, 方智远, 刘玉梅, 杨丽梅, 庄木, 孙培田. 结球甘蓝抗寒性配合力分析及优良抗寒组合选育. 中国蔬菜, 2011(14): 23-27.

Zhang Y Y, Jin Z, Fang Z Y, Liu Y M, Yang L M, Zhuang M, Sun P T. Combining ability analysis of cold-resistance characteristic and elite combination breeding in cabbage (Brassica oleracea var. capitata L.). China Vegetables, 2011(14): 23-27. (in Chinese)

[31] 吴吉祥, 王国建, 朱军, 许馥华, 季道藩. 陆地棉种子性状直接效应和母体效应的遗传分析. 作物学报, 1995, 21(6): 659-664.

Wu J X, Wang G J, Zhu J, Xu F H, Ji D F. Genetic analysis on direct and maternal effects of seed traits in upland cotton (Gossypium hirsutum L.). Acta Agronomica Sinica, 1995, 21(6): 659-664. (in Chinese)

[32] 李少钦, 王健胜, 张文学, 李殿荣, 郑磊, 田建华. 甘蓝型油菜优良亲本对杂种后代产量性状的遗传效应分析. 中国油料物学报, 2011, 33(6): 545-549.

Li S Q, Wang J S, Zhang W X, Li D R, Zheng L, Tian J H. Genetic analysis for hybrid yield traits using elite parents of Brassica napus L.. Chinese Journal of Oil Crop Sciences, 2011, 33(6): 545-549. (in Chinese)

白菜型冬油菜抗寒相关性状的遗传分析

Genetic Analysis of Traits Related to Cold Resistance in Winter Rapeseed (Brassica rapa L.)

RichHTML

PDF

赞

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	朱佩佩,罗燚佳,向雯,张明磊,张剑侠. 抗寒无核葡萄杂种胚挽救及分子标记辅助选择[J]. 中国农业科学, 2021, 54(6): 1218-1228.
[2]	任涛,郭丽璇,张丽梅,杨旭坤,廖世鹏,张洋洋,李小坤,丛日环,鲁剑巍. 我国冬油菜典型种植区域土壤养分现状分析[J]. 中国农业科学, 2020, 53(8): 1606-1616.
[3]	徐春梅,邹娅,刘自刚,米文博,徐明霞,董小云,曹小东,郑国强,方新玲. 白菜型冬油菜萌动种子低温春化的生理生化特征[J]. 中国农业科学, 2020, 53(5): 929-941.
[4]	曹小东,刘自刚,米文博,徐春梅,邹娅,徐明霞,郑国强,方新玲,崔小茹,董小云,米超,陈其鲜. 甘蓝型冬油菜北移种植的适应性分析[J]. 中国农业科学, 2020, 53(20): 4164-4176.
[5]	蒋倩红,陆志峰,赵海燕,郭俊杰,刘文波,凌宁,郭世伟. 长江中下游冬油菜产区有机无机肥配施下减氮增效潜力分析[J]. 中国农业科学, 2020, 53(14): 2907-2918.
[6]	卜容燕,程燚,王慧,韩上,胡荣根,余忠,李敏,程文龙,孙义祥,王家宝,武际. 安徽省冬油菜的施肥现状及肥料利用率[J]. 中国农业科学, 2020, 53(10): 2034-2044.
[7]	蒲媛媛,赵玉红,武军艳,刘丽君,白静,马骊,牛早霞,金姣姣,方彦,李学才,孙万仓. 北方强冬性甘蓝型冬油菜品种(系)抗寒性评价[J]. 中国农业科学, 2019, 52(19): 3291-3308.
[8]	刘秋霞, 任涛, 张亚伟, 廖世鹏, 李小坤, 丛日环, 鲁剑巍. 华中区域直播冬油菜临界氮浓度稀释曲线的建立与应用[J]. 中国农业科学, 2019, 52(16): 2835-2844.
[9]	赵雅楠,骆强伟,王跃进. 利用胚挽救技术创制无核抗寒葡萄新种质[J]. 中国农业科学, 2018, 51(21): 4119-4130.
[10]	朱芸，徐华丽，张洋洋，任涛，丛日环，鲁剑巍. 长江流域农民习惯施肥与推荐施肥的冬油菜产量与养分效率差异分析－基于大样本田间试验[J]. 中国农业科学, 2018, 51(15): 2948-2957.
[11]	张亚伟，刘秋霞，朱丹丹，樊小林，任涛，张丽梅，李小坤，丛日环，鲁剑巍. 油菜专用控释尿素用量对冬油菜产量和氮素吸收的影响[J]. 中国农业科学, 2018, 51(1): 139-148.
[12]	焦美玲，弓瑞娟，钱稷，张钢. 电阻抗断层成像评价丰花月季抗寒性[J]. 中国农业科学, 2017, 50(7): 1302-1316.
[13]	高梦烛，赵亚林，闫青地，张海丽，王凤茹，董金皋. 拟南芥PMRP基因在叶绿体淀粉粒积累和抗寒性中的作用[J]. 中国农业科学, 2016, 49(5): 832-839.
[14]	任涛，鲁剑巍. 中国冬油菜氮素养分管理策略[J]. 中国农业科学, 2016, 49(18): 3506-3521.
[15]	刘涛，鲁剑巍，任涛，汪威，王振，王少华. 适宜氮水平下冬油菜苗期不同叶位叶片光合氮分配特征[J]. 中国农业科学, 2016, 49(18): 3532-3541.