白菜黑斑病抗性鉴定及QTL定位

doi:10.3864/j.issn.0578-1752.2013.24.011

中国农业科学 ›› 2013, Vol. 46 ›› Issue (24): 5173-5179.doi: 10.3864/j.issn.0578-1752.2013.24.011

白菜黑斑病抗性鉴定及QTL定位

陈莹1, 2, 柳李旺1, 谢学文2, 孟霖2, 刘博2, 王晓武2, 李宝聚2, 武剑2

1.南京农业大学园艺学院，南京 210095；
2.中国农业科学院蔬菜花卉研究所，北京 100081

收稿日期:2013-05-13 出版日期:2013-12-16 发布日期:2013-08-21
通讯作者: 武剑，Tel：010-82105971；E-mail：wujian@caas.cn。李宝聚，Tel：010-82109518；E-mail：libaoju@caas.cn
作者简介:陈莹，Tel：010-82105971；E-mail：2010104072@njau.edu.cn
基金资助:
中央级公益性科研院所基本科研业务费专项（1610032011011）、农业部园艺作物生物学与种质创制重点实验室资助

Evaluation and QTL Mapping for Resistance to Black Spot (Alternaria brassicicola) in Brassica rapa

CHEN Ying-1, 2 , LIU Li-Wang-1, XIE Xue-Wen-2, MENG Lin-2, LIU Bo-2, WANG Xiao-Wu-2, LI Bao-Ju-2, WU Jian-2

1.College of Horticulture, Nanjing Agriculture University, Nanjing 210095;
2.Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081

Received:2013-05-13 Online:2013-12-16 Published:2013-08-21

摘要/Abstract

摘要： 【目的】鉴定白菜类作物黑斑病抗性，同时进行白菜黑斑病抗病QTL定位研究，为白菜抗黑斑病品种改良提供理论依据。【方法】以喷雾法接种芸薹生链格孢（Alternaria brassicicola）分生孢子液于30 d苗龄的白菜离体叶片或植株，黑暗保湿处理后统计病情指数，比较在接种后不同接种部位和不同保湿天数抗性的差异。以优化后的方法鉴定白菜品种的黑斑病抗性，利用白菜重组自交系（RILs）群体进行黑斑病抗性QTL定位。【结果】优化了白菜黑斑病抗性鉴定方法，并利用该方法鉴定了70份白菜类作物品种的黑斑病抗性，筛选出14份抗黑斑病材料。同时利用白菜RILs群体，定位出两个黑斑病抗性QTL位点，分别位于A05 和A06染色体上。【结论】利用离体叶法对白菜接种芸薹生链格孢，并黑暗保湿处理5 d后进行病情调查可以有效鉴定不同白菜材料对黑斑病的抗性差异，且最大程度区分材料间的抗性水平。白菜类作物中缺少对黑斑病的高抗材料。利用白菜RILs群体，在白菜A05与A06号染色体上各定位出一个黑斑病抗性QTL位点。

关键词: 白菜 , 黑斑病 , 抗性鉴定 , 离体叶 , QTL定位

Abstract: 【Objective】The aim of this study is to evaluate Brassica rapa resistance to black spot disease (Alternaria brassicicola), and map QTL related to this trait. The result will provide theoreticalbasis for genetic improvement on resistance to black spot disease in B. rapa crops.【Method】Conidia of A. brassicicola was sprayed to 30-day-old B. rapa seedlings or detached leaves. Disease index was scored after seedlings or detached leaves being kept in dark and wet for three to six days to determine the number of days for inoculation to distinguish the difference in resistance among B. rapa accessions. Using the optimized method, 70 B. rapa accessions were screened for their resistance to black spot disease. Furthermore, a population with 122 recombinant inbred lines (RILs) was used to map QTL for black spot resistance in B. rapa. 【Result】 It was found that inoculation of detached leaves for five days could well distinguish the resistance to black spot disease in B. rapa. Fourteen from 70 B. rapa accessions were identified as resistant to black spot disease. Moreover, we mapped two QTLs related to resistance to black spot in B. rapa, one of which was identified in both two experiments. 【Conclusion】The study showed that different levels of resistance to black spot disease were clearly distinguished when the detached 3rd/4th true leaves of B. rapa being inoculated with A. brassicicola conidia in dark and wet for 5 days. No accession with high resistance to black spot disease was identified in this study. Two QTLs related to resistance to black spot were mapped on chromosomes A05 and A06, respectively.

Key words: Brassica rapa , black spot , identification for resistance , detached leaf , mapping QTL

陈莹1, 2, 柳李旺1, 谢学文2, 孟霖2, 刘博2, 王晓武2, 李宝聚2, 武剑2. 白菜黑斑病抗性鉴定及QTL定位[J]. 中国农业科学, 2013, 46(24): 5173-5179.

CHEN Ying-1, 2 , LIU Li-Wang-1, XIE Xue-Wen-2, MENG Lin-2, LIU Bo-2, WANG Xiao-Wu-2, LI Bao-Ju-2, WU Jian-2. Evaluation and QTL Mapping for Resistance to Black Spot (Alternaria brassicicola) in Brassica rapa[J]. Scientia Agricultura Sinica, 2013, 46(24): 5173-5179.

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白菜黑斑病抗性鉴定及QTL定位

Evaluation and QTL Mapping for Resistance to Black Spot (Alternaria brassicicola) in Brassica rapa

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