四川马铃薯晚疫病菌交配型、生理小种、甲霜灵敏感性 及mtDNA单倍型组成分析

doi:10.3864/j.issn.0578-1752.2013.04.007

摘要/Abstract

摘要： 【目的】对马铃薯晚疫病菌群体特征进行系统分析，为马铃薯晚疫病的防控提供科学依据。【方法】对2008—2011年采集自四川的马铃薯晚疫病菌的交配型、甲霜灵敏感性、生理小种及mtDNA单倍型进行分析。【结果】四川马铃薯晚疫病菌以A2交配型为主，占测定菌株的62.5%，A1交配型和自育型菌株的发生频率分别为18.8%和18.4%，A1交配型菌株集中在九龙县和普格县。192个菌株中共测定出55个生理小种，其中生理小种1.2.3.4.5.6.7.8.9.10.11发生频率最高，99.48%的供试菌株含有多个毒力基因。甲霜灵敏感性测定发现四川马铃薯晚疫病菌群体包含抗性、中抗和敏感菌株，分别占测定菌株的63.0%、22.0%和15.0%，敏感菌株分布于普格县、道孚县和九龙县。检测到四川马铃薯晚疫病菌有Ⅰa和Ⅱa两种单倍型，分别占测定菌株的97.4%和2.6%，为第二次全球迁移后出现的“新”群体，Ⅰa单倍型马铃薯晚疫病菌广泛分布于四川各马铃薯产区，其中存在A1交配型、A2交配型、自育型和未知交配型菌株。【结论】四川马铃薯晚疫病菌群体组成日趋复杂，亟待发掘新抗源和培育水平抗病品种，以及合理布局已有抗病品种和利用马铃薯晚疫病预警系统，科学防控马铃薯晚疫病。

关键词: 马铃薯晚疫病 , 交配型 , 生理小种 , 甲霜灵敏感性 , mtDNA单倍型 , 致病疫霉

Abstract: 【Objective】The objective of this study is to provide a scientific basis to control potato late blight through the systematical analysis of populations of Phytophthora infestans. 【Method】The mating types, metalaxyl sensitivity, physiological races and mtDNA haplotypes of P. infestans isolates collected in Sichuan during 2008 to 2011 were analyzed. 【Result】Most P. infestans isolates in Sichuan were A2 mating type with the frequency of 62.5%. The frequency of A1 mating type and self-fertility in the isolates were 18.8% and 18.4%, respectively. The isolates of A1 mating type were only detected in Jiulong and Puge Counties. Fifty-five races were identified among 192 isolates and race 1.2.3.4.5.6.7.8.9.10.11 were the dominant races in Sichuan. There were 99.48% isolates contained at least two virulence genes. The sensitivity test to metalaxyl showed that resistant, intermediate and sensitive isolates were detected in Sichuan with the frequency of 63.0%, 22.0% and 15.0%, respectively. The sensitive isolates distributed in Puge, Daofu and Jiulong Counties. Two mtDNA haplotypes,Ⅰa and Ⅱa, were detected from populations of P. infestans in Sichuan with the frequency of 97.4% and 2.6%, respectively, which belonged to the “new” populations, following the second global migration of the pathogen.Ⅰa haplotype distributed in all potato producing areas extensively, including A1 mating type, A2 mating type, self-fertility and unknown mating type isolates. 【Conclusion】The composition of populations of P. infestans is getting more complex in Sichuan. To control potato late blight scientifically it is necessary to explore new resistant germplasms, breed horizontal resistant varieties, dispose resistant varieties reasonably and take full advantage of the potato late blight forecasting system.

Key words: potato late blight, mating type, physiological race, metalaxyl sensitivity, mtDNA haplotype, Phytophthora infestans

李洪浩, 彭化贤, 席亚东, 王晓黎, 刘波微. 四川马铃薯晚疫病菌交配型、生理小种、甲霜灵敏感性及mtDNA单倍型组成分析[J]. 中国农业科学, 2013, 46(4): 728-736.

LI Hong-Hao, PENG Hua-Xian, XI Ya-Dong, WANG Xiao-Li, LIU Bo-Wei. Characteristics of Mating Types, Physiological Races, Metalaxyl Sensitivity and mtDNA Haplotypes of Phytophthora infestans in Sichuan Province[J]. Scientia Agricultura Sinica, 2013, 46(4): 728-736.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2013.04.007

https://www.chinaagrisci.com/CN/Y2013/V46/I4/728

参考文献

[1]Hooker W J. Compendium of Potato Disease. St, Paul, MN: American Phytopathology Society, 1981.

[2]Hohl H R, Iselin K. Strains of Phytophthora infestans with from Switzerland A2 mating type behaviour. Transaetions of the British Mycological Soeiety, 1984, 83(3): 529-530.

[3]Fry W E, Goodwin S B, Dyer A T, Matuszak J M, Drenth A, Tooley P W, Sujkowski L S, Koh Y J, Cohen B A, Spielman L J, Deahl K L, Inglis D A, Sandlan K P. Historical and recent migrations of Phytophthora infestans: chronology, pathways, and implications. Plant Disease, 1993, 77(7): 653-661.

[4]Drenth A, Turkensteen L J, Govers F. The occurrence of the A2 mating type of Phytophthora infestans in the Netherlands; significance and consequences. Netherlands Journal of Plant Pathology, 1993, 99(Suppl. 3): 57-67.

[5]Malcolmson J F. Phytophthora infestans A2 compatibility type recorded in great Britain. Transactions of the British Mycological Society, 1985, 85(3): 531.

[6]Shaw D S, Fyfe A M, Hibberd P G, Abdel-Sattar M A. Occurrence of the rare A2 mating type of Phytophthora infestans on imported Egyptian potatoes and the production of sexual progeny with A1 mating types from the UK. Plant Pathology, 1985, 34(4): 552-556.

[7]Singh B P, Roy S, Bhattacharyya S K. Occurrence of the A2 mating type of Phytophthora infestans in India. Potato Research, 1994, 37: 227-231.

[8]黄河, 程汉清, 徐天宇, 徐大雅, 霍守祥. 我国北部马铃薯晚疫病菌生理小种的发生和变化. 植物病理学报, 1981, 11(1): 45-49.

Huang H, Cheng H Q, Xu T Y, Xu D Y, Huo S X. The occurrence and alteration of physiological races of Phytophthora infestans in northern region of China. Acta Phytopathologica Sinica, 1981, 11(1): 45-49. (in Chinese)

[9]韩彦卿, 秦宇轩, 朱杰华, 王鹤, 耿硕, 吴婧莲, 杨志辉. 2006-2008年中国部分地区马铃薯晚疫病菌生理小种的分布. 中国农业科学, 2010, 43(17): 3684-3690.

Han Y Q, Qin Y X, Zhu J H, Wang H, Geng S, Wu J L, Yang Z H. Distribution of physiological races of Phytophthora infestans on potato collected in the investigated areas of China from 2006 to 2008. Scientia Agricultura Sinica, 2010, 43(17): 3684-3690. (in Chinese)

[10]刘波微, 彭化贤, 席亚东, 何俊蓉. 四川马铃薯晚疫病菌生理小种鉴定及品种抗病评价. 西南农业学报, 2010, 23(3): 747-751.

Liu B W, Peng H X, Xi Y D, He J R. Identification of physiological race with Phytophthora infestans and resistant assessment of variety. Southwest China Journal of Agricultural Sciences, 2010, 23(3): 747-751. (in Chinese)

[11]毕朝位, 杜喜翠, 车兴壁, 马金成, 王中康. 重庆地区马铃薯晚疫病菌 (Phytophthora infestans)对甲霜灵抗性及抗性水平测定. 中国马铃薯, 2002, 16(2): 70-73.

Bi C W, Du X C, Che X B, Ma J C, Wang Z K. Resistance and resistant level of Phytophthora infestans to metalaxyl in Chongqing. Chinese Potato Journal, 2002, 16(2): 70-73. (in Chinese)

[12]Guo L, Zhu X Q, Hu C H, Ristaino J B. Genetic structure of Phytophthora infestans populations in China indicates multiple migration events. Phytopathology, 2010, 100(10): 997-1006.

[13]Vargas A M, Quesada Ocampo L M, Céspedes M C, Carreño N, González A, Rojas A, Zuluaga A P, Myers K, Fry W E, Jiménez P, Bernal A J, Restrepo S. Characterization of Phytophthora infestans populations in Colombia: first report of the A2 mating type. Phytopathology, 2009, 99(1): 82-88.

[14]赵志坚, 王淑芬, 李成云, 李先平, 何云昆. 云南省马铃薯晚疫病菌交配型分布及发生频率. 西南农业学报, 2001, 14(4): 55-57.

Zhao Z J, Wang S F, Li C Y, Li X P, He Y K. Distribution and occurrence frequency of mating type of Phytophthora infestans from potato in Yunnan. Southwest China Journal of Agricultural Sciences, 2001, 14(4): 55-57. (in Chinese)

[15]Forbes G A. Laboratory manual for Phytophthora infestans work at CIP-Quito, CIP, 2001: 1-41.

[16]Black W, Mastenbrock C, Mills W R, Peterson L C. A proposal for an international nomenclature of races of Phytophthora infestans and of genes controlling immunity in Solanum demissum derivaties. Enphytica, 1953, 2(3): 173-179.

[17]Griffith G W, Shaw D S. Polymorphisms in Phytophthora infestans: four mitochondrial haplotypes are detected after PCR amplification of DNA from pure cultures or from host lesions. Applied and Environmental Microbiology, 1998, 64(10): 4007-4014.

[18]Nishimura R, Sato K, Lee W H, Singh U P, Chang T T, Suryaningsih E, Suwonakenee S, Lumyong P, Chamswarng C, Tang W H, Shrestha S K, Kato M, Fujii N, Akino S, Kondo N, Kobayashi K, Ogoshi A. Distribution of Phytophthora infestans Populations in seven Asian countries. Annals of the Phytopathology Society of Japan, 1999, 65: 163-170.

[19]Sedegui M, Carroll R B, Morehart A L, Evans T A, Kim S H, Lakhdar R, Arifi A. Genetic structure of the Phytophthora infestans population in Morocco. Plant Disease, 2000, 84(2): 173-176.

[20]Blandón-Díaz J U, Widmark A K, Hannukkala A, Andersson B, Högberg N, Yuen J E. Phenotypic variation within a clonal lineage of Phytophthora infestans infecting both tomato and potato in Nicaragua. Phytopathology, 2012, 102(3): 323-330.

[21]黄河. 同宗配合的马铃薯晚疫病菌曾在中国被发现. 植物病理学报, 2002, 32(4): 347-350.

Huang H. Homothallic Phytophthora infestans was found in China. Acta Phytopathologica Sinica, 2002, 32(4): 347-350. (in Chinese)

[22]朱杰华, 张志铭, 李玉琴. 马铃薯晚疫病菌 (Phytophthora infestans)A2交配型的分布. 植物病理学报, 2000, 30(4): 375.

Zhu J H, Zhang Z M, Li Y Q. Distribution of the A2 mating type of potato late blight pathogen (Phytophthora infestans). Acta Phytopathologica Sinica, 2000, 30(4): 375. (in Chinese)

[23]Smart C D, Mayton H, Mizubuti E S G, Willmann M R, Fry W E. Environmental and genetic factors influencing self-fertility in Phytophthora infestans. Phytopathology, 2000, 90(9): 987-994.

[24]杨艳丽, 胡先奇, 鲁绍凤, 罗文富, Ryu K Y, 肖浪涛. 云南省马铃薯晚疫病菌生理小种的组成与分布. 华中农业大学学报, 2007, 26(3): 297-301.

Yang Y L, Hu X Q, Lu S F, Luo W F, Ryu K Y, Xiao L T. Composition and distribution of physiological race of Phytophthora infestans in Yunnan Province. Journal of Huazhong Agricultural University, 2007, 26(3): 297-301. (in Chinese)

[25]成兰芳, 王生荣. 甘肃马铃薯晚疫病菌毒力类型及分布初探. 西北农业学报, 2011, 20(11): 179-182.

Cheng L F, Wang S R. The virulence type and distribution of Phytophthora infestans in Gansu Province. Acta Agriculturae Boreali-occidentalis Sinica, 2011, 20(11): 179-182. (in Chinese)

[26]Goodwin S B, Sujkowski L S, Fry W E. Widespread distribution and probable origin of resistance to metalaxyl in clonal genotypes of Phytophthora infestans in the United States and Western Canada. Phytopathology, 1996, 86(7): 793-800.

[27]Shattock R C. Studies on the inheritance of resistance to metalaxyl in Phytophthora infestans. Plant Pathology, 1988, 37: 4-11.

[28]赵志坚, 曹继芬, 李灿辉, 孙道旺, 杨明英, 王军. 云南致病疫霉交配型、甲霜灵敏感性、mtDNA单倍型及其群体演替研究. 中国农业科学, 2007, 40(4): 727-734.

Zhao Z J, Cao J F, Li C H, Sun D W, Yang M Y, Wang J. Characteristics of mating type, metalaxyl sensitivity, mtDNA haplotype and succession of Phytophthora infestans populations in Yunnan, 2000 to 2003. Scientia Agricultura Sinica, 2007, 40(4): 727-734. (in Chinese)

[29]Guo J, van der Lee T, Qu D Y, Yao Y Q, Gong X F, Liang D L, Xie K Y, Wang X W, Govers F. Phytophthora infestans isolates from Northern China show high virulence diversity but low genotypic diversity. Plant Biology, 2009, 11(1): 57-67.

[30]连延浩, 叶广继, 王舰. 青海马铃薯晚疫病菌线粒体DNA单倍型鉴定及分析. 植物病理学报, 2012, 42(2): 167-168.

Lian Y H, Ye G J, Wang J. Identification and analysis of mitochondrial haplotypes on potato Phytophthora infestans in Qinghai area. Acta Phytopathologica Sinica, 2012, 40(2): 164-168. (in Chinese)