中国农业科学 ›› 2013, Vol. 46 ›› Issue (4): 728-736.doi: 10.3864/j.issn.0578-1752.2013.04.007

• 植物保护 • 上一篇    下一篇

四川马铃薯晚疫病菌交配型、生理小种、甲霜灵敏感性 及mtDNA单倍型组成分析

 李洪浩, 彭化贤, 席亚东, 王晓黎, 刘波微   

  1. 四川省农业科学院植物保护研究所/农业部西南作物有害生物综合治理重点实验室,成都610066
  • 收稿日期:2012-09-04 出版日期:2013-02-15 发布日期:2012-10-08
  • 通讯作者: 通信作者刘波微,Tel:028-84590050;E-mail:liubowei2005@hotmail.com
  • 作者简介:李洪浩,Tel:028-84590050;E-mail:leehh071@126.com
  • 基金资助:

    四川省“十二五”育种公关项目(2011NZ0098-17)

Characteristics of Mating Types, Physiological Races, Metalaxyl Sensitivity and mtDNA Haplotypes of Phytophthora infestans in Sichuan Province

 LI  Hong-Hao, PENG  Hua-Xian, XI  Ya-Dong, WANG  Xiao-Li, LIU  Bo-Wei   

  1. Key Laboratory of Integrated Pest Management on Crops in Southwest, Ministry of Agriculture, Institute of Plant Protection, Sichuan Academy of Agricultural Science, Chengdu 610066
  • Received:2012-09-04 Online:2013-02-15 Published:2012-10-08

摘要: 【目的】对马铃薯晚疫病菌群体特征进行系统分析,为马铃薯晚疫病的防控提供科学依据。【方法】对2008—2011年采集自四川的马铃薯晚疫病菌的交配型、甲霜灵敏感性、生理小种及mtDNA单倍型进行分析。【结果】四川马铃薯晚疫病菌以A2交配型为主,占测定菌株的62.5%,A1交配型和自育型菌株的发生频率分别为18.8%和18.4%,A1交配型菌株集中在九龙县和普格县。192个菌株中共测定出55个生理小种,其中生理小种1.2.3.4.5.6.7.8.9.10.11发生频率最高,99.48%的供试菌株含有多个毒力基因。甲霜灵敏感性测定发现四川马铃薯晚疫病菌群体包含抗性、中抗和敏感菌株,分别占测定菌株的63.0%、22.0%和15.0%,敏感菌株分布于普格县、道孚县和九龙县。检测到四川马铃薯晚疫病菌有Ⅰa和Ⅱa两种单倍型,分别占测定菌株的97.4%和2.6%,为第二次全球迁移后出现的“新”群体,Ⅰa单倍型马铃薯晚疫病菌广泛分布于四川各马铃薯产区,其中存在A1交配型、A2交配型、自育型和未知交配型菌株。【结论】四川马铃薯晚疫病菌群体组成日趋复杂,亟待发掘新抗源和培育水平抗病品种,以及合理布局已有抗病品种和利用马铃薯晚疫病预警系统,科学防控马铃薯晚疫病。

关键词: 马铃薯晚疫病 , 交配型 , 生理小种 , 甲霜灵敏感性 , mtDNA单倍型 , 致病疫霉

Abstract: 【Objective】The objective of this study is to provide a scientific basis to control potato late blight through the systematical analysis of populations of Phytophthora infestans. 【Method】The mating types, metalaxyl sensitivity, physiological races and mtDNA haplotypes of P. infestans isolates collected in Sichuan during 2008 to 2011 were analyzed. 【Result】Most P. infestans isolates in Sichuan were A2 mating type with the frequency of 62.5%. The frequency of A1 mating type and self-fertility in the isolates were 18.8% and 18.4%, respectively. The isolates of A1 mating type were only detected in Jiulong and Puge Counties. Fifty-five races were identified among 192 isolates and race 1.2.3.4.5.6.7.8.9.10.11 were the dominant races in Sichuan. There were 99.48% isolates contained at least two virulence genes. The sensitivity test to metalaxyl showed that resistant, intermediate and sensitive isolates were detected in Sichuan with the frequency of 63.0%, 22.0% and 15.0%, respectively. The sensitive isolates distributed in Puge, Daofu and Jiulong Counties. Two mtDNA haplotypes,Ⅰa and Ⅱa, were detected from populations of P. infestans in Sichuan with the frequency of 97.4% and 2.6%, respectively, which belonged to the “new” populations, following the second global migration of the pathogen.Ⅰa haplotype distributed in all potato producing areas extensively, including A1 mating type, A2 mating type, self-fertility and unknown mating type isolates. 【Conclusion】The composition of populations of P. infestans is getting more complex in Sichuan. To control potato late blight scientifically it is necessary to explore new resistant germplasms, breed horizontal resistant varieties, dispose resistant varieties reasonably and take full advantage of the potato late blight forecasting system.

Key words: potato late blight, mating type, physiological race, metalaxyl sensitivity, mtDNA haplotype, Phytophthora infestans