不同基因型烤烟质体色素降解及与烤后烟叶挥发性降解物含量关系

doi:10.3864/j.issn.0578-1752.2012.16.014

摘要/Abstract

摘要： 【目的】研究不同基因型烤烟成熟和调制过程中色素降解及与烤后烟叶中性挥发性色素降解成分含量的关系。【方法】采用液相色谱方法测定豫中浓香型烟区9个不同基因型烤烟中部叶质体色素含量。【结果】在成熟和调制过程中烟叶的质体色素含量呈下降趋势，叶绿素的总降解量总体大于类胡萝卜素，且叶绿素a的降解量显著大于叶绿素b，在调制后烟叶中叶绿素b残留量较多，叶绿素在烟叶成熟期的降解量大于在调制期间的降解量。在类胡萝卜素中叶黄素含量和降解量最大，且在成熟期间的降解量大于调制期间的降解量，新黄质在成熟期的降解量大于调制期间的降解量，但β-胡萝卜素和紫黄质在调制期间的降解量大于成熟期间的降解量。不同基因型色素降解量不同，且与调制后烟叶色素降解类中性香气成分含量多呈显著正相关关系，与调制后烟叶色素含量无显著相关性或呈负相关关系。类胡萝卜素降解产物总量和许多重要香气成分与成熟期色素降解量的相关性大于与调制期降解量的相关性。【结论】烟叶成熟过程中叶绿素、叶黄素和新黄质的降解量大于调制期的降解量。烤后烟叶中挥发性色素降解香气成分含量与质体色素的降解量，特别是成熟期色素降解量有密切关系，提高烟叶成熟度对促进烟叶香气物质形成至关重要。

关键词: 烤烟, 基因型, 质体色素, 降解量, 降解产物

Abstract: 【Objective】 The research was conducted to investigate the changes in plastid pigment contents during middle leaf mature and curing, and the relationship of pigment degradation amount and rate to volatile pigment catabolite content in cured leaves. 【Method】HPLC method was used to measure the pigment and volatile aroma components in 9 genotypes of flue-cured tobacco grown in central Henan Province. 【Result】The results showed that the plastid pigment contents decreased during leaf maturing and curing, with the total degradation amount and rate of chlorophyll being greater than that of carotenoids, and that of chlorophyll a being significantly greater than that of chlorophyll b, resulting in relatively higher amount of residue of chlorophyll b in cured leaves. The degradation rate of chlorophyll during leaf maturing was greater than that during curing. Among the carotenoids, lutein had the highest content and greatest degradation amount, and that during leaf maturing was greater than that during curing. The degradation rate of neoxanthin during maturing was also greater that during curing, while it was the contrary for β-carotene and violaxanthin. Different genotypes had different pigment degradation amounts and had significantly positive correlations with the contents of volatile degraded aroma compounds in cured leaves, while there was no or negative correlations with the contents of pigment residue in cured leaves. Total amount of pigment-degraded aroma compounds and the contents of most important individual aroma components had greater positive correlations with degradation amount during maturing than that during curing. 【Conclusion】 The degradation amount of chlorophyll, luten and neoxanthin were greater than that during curing. Total amount of pigment degraded aroma compounds had a close relationship with the pigment degradation amount, especially that during maturing. Thus the increase of maturity of tobacco was pivotal in promoting the formation of aroma components in tobacco leaves.

Key words: flue-cured tobacco, genotype, plastid pigment, degradation amount, catabolite

史宏志, 顾少龙, 段卫东, 王建安, 钱华, 刘清华, 苏菲, 刘国顺. 不同基因型烤烟质体色素降解及与烤后烟叶挥发性降解物含量关系[J]. 中国农业科学, 2012, 45(16): 3346-3356.

SHI Hong-Zhi, GU Shao-Long, DUAN Wei-Dong, WANG Jian-An, QIAN Hua, LIU Qing-Hua, SU Fei, LIU Guo-Shun. Degradation of Plastid Pigment and Its Relationship with Volatile Catabolite Content in Cured Leaves of Different Genotypes of Flue-Cured Tobacco[J]. Scientia Agricultura Sinica, 2012, 45(16): 3346-3356.

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参考文献

[1]Tso T C. Production, Physiology and Biochemistry of Tobacco Plant. Beltsville, Maryland, USA: IDEALS Inc. 1990.

[2]王瑞新. 烟草化学. 北京: 中国农业出版社, 2003: 84-85.

Wang R X. Tobacco Chemistry. Beijing: China Agriculture Press, 2003:84-85.(in Chinese)

[3]金闻博, 戴亚, 横田拓. 烟草化学. 北京: 清华大学出版社, 2000: 45-46.

Jin W B, Dai Y, Heng T. Tobacco Chemistry. Beijing: Tsinghua University Press, 2000: 45-46. (in Chinese)

[4]史宏志, 刘国顺, 杨惠娟，姬小明. 北京:中国农业出版社, 2011.

Shi H Z, Liu G S, Yang H J, Ji X M. Tobacco Aroma. Beijing：Chinese Agricultural Press,1998. (in Chinese)

[5]Week W W. Chemistry of tobacco constituents influencing flavor and aroma. Recent Advance in Tobacco Science, 1985, (11): 175-200.

[6]杨虹琦, 周冀衡, 罗泽民, 杨述元.烟叶质体色素代谢与香味物质形成关系的研究.西南农业大学学报: 自然科学版, 2004, 26(5):640-644.

Yang H Q, Zhou J H, Luo Z M, Yang S Y. Study on chromoplast pigments and its degrading products of the flue-cured tobacco from different producing regions in China. Journal of Southwest Agricultural University: Natural Science, 2004, 26(5): 640-644. (in Chinese)

[7]许自成, 张婷, 马国华, 马京民, 程昌新, 黄平俊. 不同调控措施对烤烟质体色素及其降解产物的影响. 河南农业大学学报, 2006, 40(1): 15-17, 26.

Xu Z C, Zhang T, Ma G H, Ma J M, Cheng C X, Huang P J. Effect of different agronomic measures on chromoplast pigment and its degraded products in flue-cured tobacco leaf. Journal of Henan Agricultural University, 2006, 40(1): 15-17, 26. (in Chinese)

[8]韦凤杰, 王芳, 刘国顺, 李亚娟, 郭桥燕. 烤烟叶片发育过程中类胡萝卜素的组分含量变化研究. 河南农业大学学报, 2006, 40(6): 588-591.

Wei F J, Wang F, Liu G S, Li Y J, Guo Q Y. Research on metabolism of flue-cured tobacco leaf carotenoids during its growth development. Journal of Henan Agricultural University, 2006, 40(6): 588-591. (in Chinese)

[9]史宏志, 韩锦峰, 刘卫群, 范艺宽, 刘清华. 氮素营养对烤烟脂类化合物含量和脂氧酶活性的影响.中国烟草学报, 1997, 3(4):41-48.

Shi H Z, Han J F, Liu W Q, Fan Y K, Liu Q H. Effects of nitrogen nutrition on lipid content and lipoxygenese activity in flue-cured tobacco leaves. Acta Tabacaria Sinica, 1997, 3(4):41-48. (in Chinese)

[10]赵进恒, 赵铭钦, 韩富根, 李元实, 金洪石, 刘友杰, 宋鹏飞, 张迪. 水肥耦合对烤烟质体色素及其降解产物的影响. 华北农学报，2010, 25 (2): 216-220.

Zhao J H, Zhao M Q, Han F G, Li Y S, Jin H S, Liu Y J, Song P F, Zhang D. Effect of water and fertilizer coupling on chromoplast pigment and its degraded products in flue-cured tobacco leaf. Acta Agriculturae Boreali-Sinica, 2010, 25 (2): 216-220. (in Chinese)

[11]常爱霞, 贾兴华, 郝廷亮, 徐玉田, 毕建立, 厉昌坤,王允白.特香型烤烟挥发性致香物质的测定与分析. 中国烟草科学, 2002(1): 1-5.

Chang A X, Jia X H, Hao T L, Xu Y T, Bi J L, Li C K, Wang Y B. Identification and analysis of volatile aroma and flavor constituents of flue-cured tobacco with special flavoring aroma. Chinese Tobacco Science, 2002(1): 1-5. (in Chinese)

[12]史宏志，李志，刘国顺, 王道支，祖朝龙，王达洲，杨永锋. 皖南不同质地土壤烤后烟叶中性香气成分含量及焦甜香风格的差异. 土壤，2009, 41 (6): 980-985.

Shi H Z, Li Z, Liu G S, Wang D Z, Zu C L, Wang D Z, Yang Y F. Differences in contents of neutral aroma components and sensory evaluation of sweet aroma in soils with different textures in south Anhui. Soils, 2009, 41(6): 980-985. (in Chinese)

[13]王新发, 杨铁钊, 殷全玉, 张小全, 刘培玉, 任庆成. 氮用量对烟叶质体色素及中性香气基础物质的影响. 华北农学报, 2010, 25 (1 ): 185-189.

Wang X F, Yang T Z, Yin Q Y, Zhang X Q, Liu P Y, Ren Q C. Effects of nitrogen level on chromoplast pigment and neutral aroma components in the leaves of flue-cured tobacco. Acta Agriculturae Boreali-Sinica, 2010, 25 (1): 185-189. (in Chinese)

[14]王芳，凌爱芬，刘国顺，舒俊生，李兰周，张正阳，杨超，李霞. 饼肥对烤烟不同发育时期类胡萝卜素及其主要产物的影响. 中国烟草学报，2007, 13(5): 44-49.

Wang F, Ling A F, Liu G S, Shu J S, Li L Z, Zhang Z Y, Yang C, Li X. Effects of cake fertilizer on content of carotenoids and major carotenoid degrading products in different phases of flue-cured tobacco, Acta Tabacaria Sinica, 2007, 13(5):44-49. (in Chinese)

[15]刘洪华，赵铭钦，王付峰，张学杰，吕中显，张迎东. 有机无机配施对烤烟质体色素及降解产物的影响. 中国烟草科学, 2010, 31(5): 38-42.

Liu H H, Zhao M Q, Wang F F, Zhang X J, Lu Z X, Zhang Y D. Effects of combined application of organic and chemical fertilizers on plastid pigment and its degraded products in flue-cured tobacco, Chinese Tobacco Science, 2010,31(5): 38-42. (in Chinese)

[16]杨虹琦, 周冀衡, 杨述元, 王勇, 周清明, 罗泽民. 不同产区烤烟中主要潜香型物质对评吸质量影响的研究.湖南农业大学学报: 自然科学版, 2005, 31(1): 11-14.

Yang H Q, Zhou J H,Yang S Y, Wang Y, Zhou Q M，Luo Z M. Effect on chiefly latent fragrant substance on panel test flue-cured tobacco from different producing area. Journal of Hunan Agricultural University: Natural Sciences, 2005, 31(1): 11-14. (in Chinese)

[17]Roberts D L, Rode W A. Isolation and identification of flavor components of burley tobacco. Tobacco Science, 1972, 16: 107-112.

[18]过伟民, 尹启生, 宋纪真, 蔡宪杰, 王信民, 王太运, 马要好. 烤烟质体色素含量的品种间差异及其与感官质量的关系.烟草科技, 2009(8): 53-59.

Guo W M, Yin Q S, Song J Z, Cai X J, Wang X M, Wang T Y, Ma Y H. Difference of plastid pigment content among tobacco cultivars and its relationship with smoking quality. Tobacco Science& Technology, 2009(8):53-59.(in Chinese)

[19]过伟民, 李伟观, 刘　阳, 宋纪真, 蔡宪杰, 王信民, 尹启生.烤烟类胡萝卜素含量与香气质量的关系. 烟草科技, 2010(1): 51-55.

Guo W M, Li W G, Liu Y, Song J Z, Cai X J, Wang X M, Yin Q S. Relationship between carotenoid content and aromatic quality and quantity of flue-cured tobacco. Tobacco Science & Technology, 2010(1): 51-55. (in Chinese)

[20]Lloyd R A. Flue-cured tobacco flavor .I. Essence and essential oil components. Tobacco Science,1976, 20: 40-48.

[21]Burton H R, Kasperbauer M J. Changes in chemical composition of tobacco laminar during senescence and curing .1.Plastid pigments .Journal of Agricultural and Food Chemistry, 1985, 33: 879-883.

[22]Whitefield D M, Rowan K S .Changes in the chlorophylls and carotenoids of leaves of tobacco during senescence. Phytochemistry, 1974, 13: 77-83.

[23]Court W A, Hendel J G. Changes in leaf pigments during senescence of flue-cured tobacco. Canadian Journal of Plant Science, 1984, 64: 229-232.

[24]景延秋, 宫长荣, 张月华, 刘晓萍, 李炎强. 烟草香味物质分析研究进展.中国烟草科学, 2005, (2): 44-48.

Jing Y Q, Gong C R, Zhang Y H, Liu X P, Li Y Q. Research progress of aroma compositions of tobacco .Chinese Tobacco Science, 2005, (2): 44-48. (in Chinese)

[25]宋朝鹏，武圣江，高远，许自成，张卫建，宫长荣.烤烟密集烘烤变黄期类胡萝卜素及其降解香气成分的变化. 中国农业科学, 2010, 43(20): 4246-4254.

Song C P, Wu S J, Gao Y, Xu Z C, Zhang W J, Gong C R. The degradation mechanism of carotenoids in flue-cured tobacco and the changes of the related aroma components in the yellowing stage during the bulk curing process. Scientia Agricultura Sinica, 2010, 43(20): 4246-4254. (in Chinese)