硫胺素和硫水平对山羊人工瘤胃代谢和微生物菌群的影响

doi:10.3864/j.issn.0578-1752.2012.08.015

摘要/Abstract

摘要： 【目的】探讨在不同硫水平条件下，添加硫胺素（维生素B1）对人工瘤胃内营养物质消化代谢的影响。【方法】按2×2因子试验设计，因子一为不同的氮硫比（高硫水平，即N﹕S=5﹕1；低硫水平即N﹕S=15﹕1），因子二为不添加和添加硫胺素（0和60 mg•kg-1）进行人工瘤胃培养试验。【结果】添加硫胺素对pH和NH3-N浓度的影响不显著；高硫水平能够抑制细菌蛋白的合成，显著提高了瘤胃培养液中原虫和细菌蛋白质的比值（P/WB）（P＜0.05）；在低硫水平下，添加硫胺素处理组TVFA的摩尔浓度和丁酸百分含量均高于不添加硫胺素组（P＜0.05），而乙酸低于不添加硫胺素组。在高硫水平下，添加硫胺素处理组与不添加组之间总挥发性脂肪酸和单一脂肪酸的摩尔浓度无显著差异（P＞0.05）。高硫水平能够降低培养液中硫胺素的总量，而添加的硫胺素则增加了培养液中硫胺素的总量；高硫水平可以降低乳酸的浓度；同一氮硫水平下，添加硫胺素处理组的牛链球菌的相对数量比非添加组分别降低了5.90%（P＜0.05）和2.05%（P＜0.01）；添加不同水平的硫和硫胺素对溶纤维丁酸弧菌的相对数量的影响不显著（P＞0.05）。【结论】人工瘤胃中硫水平对瘤胃发酵有一定影响，高硫水平可显著降低培养液中的乳酸浓度，提高原虫蛋白质的合成量，而抑制细菌蛋白质的合成；无机硫和硫胺素在降低瘤胃内乳酸浓度和缓解瘤胃酸中毒中起重要作用，并且二者对乳酸浓度的影响存在互作效应。添加不同水平的硫和硫胺素能够降低瘤胃液中牛链球菌的相对数量，对瘤胃液中溶纤维丁酸弧菌的相对数量的影响不显著（P＜0.05）。

关键词: 硫胺素, 瘤胃发酵, 瘤胃微生物

Abstract: 【Objective】An experiment was conducted to determine the effect of thiamin on rumen fermentation and microbial community under different nitrogen levels to sulfur ratio in Rusitec. 【Method】 A 2×2 two factorial design of an experiment was adopted: one factor was different nitrogen levels to sulphur ratios (5﹕1, 15﹕1), the other was different substrates with and without adding thiamin (0 and 60 mg•kg-1). 【Result】 The results showed that there was no significant effect on pH and ammonia concentration in the substrates. The high sulfur level restrained the synthesis of bacterial protein and significantly decreased the ratio of protozoal protein and bacterial protein (P＜0.05). There was no effect on the concentrations of acetate, propionate and the acetate/propionate ratio (P＜0.05). The low sulfur level increased the concentration of butyrate, total VFAs and the proportion of butyrate, and the high S level reduced the total concentration of thiamin while the total thiamin was increased by adding thiamin. There was an obvious consequence that the high sulfur substrate declined the concentration of the lactate in Rusitec. Compared to the without adding thiamin group, the relative number of Strepococcus bovis in substrates with adding thiamin decreased by 5.90% (P＜0.05) and 2.05% (P＜0.01) under the same nitrogen/sulfur ratios, and there was no significant effect on the number of Butyrivibrio fibrisovens. 【Conclusion】 It was concluded that rumen fermentation was response to sulfur level in Rusitec, higher levels of sulfur in sulfur substrate could decline the concentration of the lactate and bacterial protein while protozal protein dropped in Rusitec. It was found that sulfur and thiamin play an important role in suppressing of subacute rumen acidosis (SARA). Adding thiamin in Rusitec resulted in the decrease of the relative number of S. bovis in substrates, but had no significant effect on the number of B. fibrisovens.

Key words: thiamin, rumen fermentation, rumen micro-organisms

王洪荣, 张红伟. 硫胺素和硫水平对山羊人工瘤胃代谢和微生物菌群的影响[J]. 中国农业科学, 2012, 45(8): 1595-1605.

WANG Hong-Rong, ZHANG Hong-Wei. Effect of Thiamin on the Rumen Fermentation and Microbial Community of the Substrates in Rusitec[J]. Scientia Agricultura Sinica, 2012, 45(8): 1595-1605.

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参考文献

[1]Girard C L. B-complex vitamins for dairy cows: a new approach. Canadian Journal of Animal Science, 1998, 78: 71-90.

[2]Shaver R D, Bal M A. Effect of dietary thiamin supplementation on milk production by dairy cows. Journal of Dairy Science, 2000, 83(10): 2335-2340.

[3]de Oliveira L A, Jean-Blain C, Komisarczuk-Bony S, Durix A, Durier C. Microbial thiamin metabolism in the rumen simulating fermenter (RUSITEC): the effect of acidogenic conditions, a high sulfur level and added thiamin. British Journal of Nutrition, 1997, 78(4): 599-613.

[4]赵芸君, 孟庆翔. B族维生素在瘤胃中的合成及影响因素. 动物营养学报, 2007, 19(增刊): 492-497.

Zhao Y J, Meng Q X. Vitamin B synthesis in the rumen and their affecters. Chinese Journal of Animal Nutrition, 2007, 19(Suppl.): 492-497. (in Chinese)

[5]Tafaj M, Schollenberger M, Feofilowa J, Zebeli Q, Steingass H, Drochner W. Relationship between thiamine concentration and fermentation patterns in the rumen fluid of dairy cows fed with graded concentrate levels. Journal of Animal Physiology and Animal Nutrition, 2006, 90(7/8): 335-343.

[6]Menke K H, Steingass H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 1988, 28: 7-55.

[7]Martin-Orúe S M, Balcells J, Zakraoui F, Castrillo C. Quantification and chemical composition of mixed bacteria harvested from solid fractions of rumen digesta: effect of detachment procedure. Animal Feed Science and Technology, 1998, 71(3/4): 269-282.

[8]Czerkawshi J W. Chemical composition of microbial matter in rumen. Journal of Science in Food and Agriculture, 1976, 27(7): 621-632.

[9]Broderiek G A, Kang J H. Automated simultaneous determination of ammonia and amino acids isolation of the rumen bacteria. Applied Microbiology, 1980, 14: 794-799.

[10]熊本海, 卢德勋, 高俊. 绵羊瘤胃VFA吸收效率及模型参数的研究. 动物营养学报, 1999, 11(增刊): 248-255.

Xun B H, Lu D X, Gao J. Studies on absorption of VFA and modeling in the rumen of sheep. Chinese Journal of Animal Nutrition, 1999, 11(Suppl.): 248-255. (in Chinese)

[11]张龙翔, 张庭芳, 李令媛. 生化实验方法和技术. 第二版. 北京：高等教育出版社, 1997: 422-428.

Zhang L X, Zhang T F, Li L Y. Procedures and Techniques of Bio-Chemistry Experiments. 2nd ed. Beijing: Higher Education Express, 1997: 422-428. (in Chinese)

[12]Zhou J, Bruns M A, Tiedje J M. DNA recovery from soils of diverse composition. Applied and Environmental Microbiology, 1996, 62(2): 316-322.

[13]Tajima K, Aminov R I, Nagamine T, Matsui H, Nakamura M, Benno Y. Diet-dependent shift in the bacterial population of the rumen revealed with real-time PCR. Applied and Enviromental Microbiology, 2001, 67(6): 2766-2774.

[14]Martin S A. Potential Form Manipulating Gastrointestinal Microflora: Alternatives to Antibiotics and Ionospheres in Proceeding 29th. Annual Georgia Nutrition Conference for the Feed Industry, 1992.

[15]郑拉弟. 无机硫对绵羊瘤胃微生物数量及氮硫代谢的影响[D]. 呼和浩特: 内蒙古农业大学, 2006.

Zhen L D. Effect of sulfur on microbial community and nitrogen metabolism in the rumen of sheep[D]. Huhhot: Inner Mongolian Agricultural University, 2006. (in Chinese)

[16]van Houtert M. Challenging the retinal for altering VFA ratios in growing ruminates. Feed Mix, 1996, 4(1): 514-525.

[17]Hegarty R S, Nolan J V, Leng R A. The effects of protozoa and of supplementation with nitrogen and sulfur on digestion and microbial metabolism in the rumen of sheep. Australian Agriculture Research, 1994, 45(6): 1215-1227.

[18]Hume I D, Moir R J, Somers M. Synthesis of microbial protein in the rumen. I. Influence of the level of nitrogen intake. Australian Journal of Agricultural Research, 1970, 21: 283-296.

[19]de Oliveira A L, Jean-Blain C, dal Corso V, Bénard V, Durix A, Komisarczuk-Bony S. Effect of a high sulphur diet on the rumen microbial activity and rumen thiamin status in sheep receiving a semi-synthetic, thiamin-free diet. Reproduction, Nutrition, Development, 1996, 36: 31-42.

[20]Breves G S, Holler H N. In vitro net synthesis of microbial protein and thiamin in rumen contents of sheep adapted to a protein-free diet: effects of varying levels of substrate and sulphur. Journal of Animal Physiology and Animal Nutrition, 1978, 41: 8-17.

[21]Olkowski A A, Laarveld B, Patience J F, Francis S I, Christensen D A. The effect of sulphate on thiamine-destroying activity in rumen content cultures in vitro. International Journal for Vitamin and Nutrition Research, 1996, 63(1): 38-44.

[22]Hungate R E, Dougherty R W, Bryant M P. Microbiological and physiological changes associated with acute indigestion in sheep. Cornell Veterinary, 1952, 4(4): 423-426.

[23]Russell J B, Hino T. Regulation of lactate production in Streptococcus bovis: a spiraling effect that contributes to rumen acidosis. Journal of Dairy Science, 1985, 68(7): 1712-1721.