可同化氮素对葡萄酒发酵香气影响研究进展

doi:10.3864/j.issn.0578-1752.2011.24.011

摘要/Abstract

摘要： 可同化氮素是葡萄酒发酵中酵母所需的重要营养物质之一，葡萄汁中可同化氮素含量低不仅导致酵母菌数量低，增加发酵延缓和中止的危险性，同时也会导致葡萄酒发酵过程中高级醇、支链酸、支链酸乙酯、H2S、挥发性硫醇等含量增加,影响葡萄酒感官质量的评价。葡萄汁中适量的可同化氮素会增加中链脂肪酸、中链脂肪酸酯、乙酸酯的含量；但含量过高会伴随生物胺和氨基甲酸乙酯的产生。本文综述了可同化氮素在葡萄酒发酵过程中对发酵香气物质包括高级醇、酯类、挥发性脂肪酸和挥发性硫化物含量及对葡萄酒感官质量评价影响等方面的最新研究进展。

关键词: 可同化氮素, 葡萄酒发酵, 香气成分, 感官评价

Abstract: Assimilable nitrogen is one of the important nutrient for yeast in wine fermentation. Low assimilable nitrogen content in grape must can not only causes yeast in low numbers and increases risk of sluggish or stuck fermentations, but also increases the production of higher alcohols, branched acids, branched acids esters, hydrogen sulfide and volatile thiols in alcohol fermentation, thus affecting wine sensory evaluation. The proper quantity of assimilable nitrogen in must can lead to the increase of medium chain fatty acid and medium chain fatty acid ester, and acetate esters content. However, productions of biogenic amines and ethyl carbamate are also associated with too high yeast-assimilable nitrogen (YAN). Recent progress in research of the effects of assimilable nitrogen on formation of aromatic compounds such as higher alcohols, esters, volatile fatty acids, volatile sulfur compounds and wine sensory evaluation were reviewed in this article.

Key words: assimilable nitrogen, wine fermentation, aroma compounds, sensory evaluation

惠竹梅, 吕万祥, 刘延琳. 可同化氮素对葡萄酒发酵香气影响研究进展[J]. 中国农业科学, 2011, 44(24): 5058-5066.

HUI Zhu-Mei, LU Wan-Xiang , LIU Yan-Lin. Advances in Research of the Effects of Assimilable Nitrogen on Formation of Aromatic Compounds in Wine Fermentation[J]. Scientia Agricultura Sinica, 2011, 44(24): 5058-5066.

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参考文献

[1]李华, 王华, 袁春龙, 王树生. 葡萄酒化学. 北京: 科学出版社, 2005.

Li H, Wang H, Yuan C L, Wang S S. Wine Chemistry. Beijing: Science Press, 2005. (in Chinese)

[2]Palomo E S, González-Viñas M A, Díaz-Maroto M C, Soriano-Pérez A, Pérez-Coello M S. Aroma potential of Albillo wines and effect of skin-contact treatment. Food Chemistry, 2007, 103: 631-640.

[3]Pérez-Coello M S, Briones Pérez A I, Ubeda Iranzo J F, Martin Alvarez P J. Characteristics of wines fermented with different Saccharomyces cerevisiae strains isolated from the La Mancha region. Food Microbiology, 1999, 16: 563-573.

[4]Regodón Mateos J A, Pérez-Nevado F, Ramírez Fernández M. Influence of Saccharomyces cerevisiae yeast strain on the major volatile compounds of wine. Enzyme and Microbial Technology, 2006, 40: 151-157.

[5]Beltran G, Novo M, Guillamón J M, Mas A, Rozès N. Effect of fermentation temperature and culture media on the yeast lipid composition and wine volatile compounds. International Journal of Food Microbiology, 2008, 121: 169-177.

[6]D’Amato D, Corbo M R, Nobile M A, Sinigaglia M. Effects of temperature, ammonium and glucose concentrations on yeast growth in a model wine system. International Journal of Food Science and Technology, 2006, 41: 1152-1157.

[7]Hernández-Orte P, Ibarz M J, Cacho J, Ferreira V. Addition of amino acids to grape juice of the Merlot variety: Effect on amino acid uptake and aroma generation during alcoholic fermentation. Food Chemistry, 2006, 98: 300-310.

[8]Beltran G, Esteve-Zarzoso B, Rozès N, Mas A, Guillamón J M. Influence of the timing of nitrogen additions during synthetic grape must fermentations on fermentation kinetics and nitrogen consumption. Journal of Agricultural and Food Chemistry, 2005, 53: 996-1002.

[9]Arias-Gil M, Garde-Cerdán T, Ancín-Azpilicueta C. Influence of addition of ammonium and different amino acid concentrations on nitrogen metabolism in spontaneous must fermentation. Food Chemistry, 2007, 103: 1312-1318.

[10]Mendes-Ferreira A, Mendes-Faia A, Leāo C. Growth and fermentation patterns of Saccharomyces cerevisiae under different ammonium concentrations and its implications in winemaking industry. Journal of Applied Microbiology, 2004, 97: 540-545.

[11]Ugliano M, Henschke P A, Herderich M J, Pretorius I S. Nitrogen management is critical for wine flavour and style. Australian Wine Research Institute Report, 2007, 22(6): 24-30.

[12]Bell S J, Henschke P A. Implications of nitrogen nutrition for grapes, fermentation and wine. Australian Journal of Grape and Wine Research, 2005, 11: 242-295.

[13]Barbosa C, Falco V, Mendes-Faia A, Mendes-Ferreira A. Nitrogen addition influences formation of aroma compounds, volatile acidity and ethanol in nitrogen deficient media fermented by Saccharomyces cerevisiae wine strains. Journal of Bioscience and Bioengineering, 2009, 108(2): 99-104.

[14]Hernández-Orte P, Bely M, Cacho J, Ferreira V. Impact of ammonium additions on volatile acidity, ethanol, and aromatic compound production by different Saccharomyces cerevisiae strains during fermentation in controlled synthetic media. Australian Journal of Grape and Wine Research, 2006, 12: 150-160.

[15]Ugliano M, Siebert T, Mercurio M, Capone D, Henschke P A. Volatile and color composition of young and model-aged Shiraz wines as affected by diammonium phosphate supplementation before alcoholic fermentation. Journal of Agricultural and Food Chemistry, 2008, 56: 9175-9182.

[16]Stines A P, Grubb J, Gockkowiak H, Henschke P A, HØj P B, Heeswijck R. Proline and arginine accumulation in developing berries of Vitis vinifera L. in Australian vineyards: Influence of vine cultivar, berry maturity and tissue type. Australian Journal of Grape and Wine Research, 2000, 6: 150-158.

[17]Callejón R M, Troncoso A M, Morales M L. Determination of amino acids in grape-derived products: A review. Talanta, 2010, 81: 1143-1152.

[18]Lee J, Paul Schreiner R P. Free amino acid pro?les from ‘Pinot noir’ grapes are in?uenced by vine N-status and sample preparation method. Food Chemistry, 2010, 119: 484-489.

[19]惠竹梅，张振文，马新丽，马少青，李华. 行间生草对赤霞珠葡萄与葡萄酒含氮化合物的影响. 中国农业科学，2010, 43(19): 4045-4052.

Xi Z M, Zhang Z W, Ma X L, Ma S Q, Li H. Effects of vineyard cover Crops on main nitrogen compounds in grape berry and wine from Vitis vinifera L. cv. Cabernet Sauvignon. Scientia Agricultura Sinica, 2010, 43(19): 4045-4052. (in Chinese)

[20]惠竹梅，李华，刘延琳，任玉巧. 行间播种紫花苜蓿对赤霞珠葡萄果实及葡萄酒含氮化合物的影响. 西北农林科技大学学报:自然科学版，2007, 35(4): 129-133.

Xi Z M, Li H, Liu Y L, RenY Q. Effect of vineyard grass covering with alfalfa (Medicago sativa) on nitrogenous compound of grape berry and wine in grape cultivar cabernet sauvignon. Journal of Northwest A & F University: Natural Science Edition, 2007, 35(4): 129-133. (in Chinese)

[21]惠竹梅，周起. 葡萄果实成熟过程中含氮化合物的变化. 中外葡萄与葡萄酒，2010(11): 8-11.

Xi Z M, Zhou Q. The changes of nitrogen compounds during maturing of grape berry. Sino-Overseas Grapevine & Wine, 2010(11): 8-11. (in Chinese)

[22]Blateyron L, Sablayrolles J M. Stuck and slow fermentations in enology: Statistical study of causes and effectiveness of combined additions of oxygen and diammonium phosphate. Journal of Bioscience and Bioengineering, 2001, 91: 184-189.

[23]Moreira N, Mendes F, Pereira O, Pinho P G, Hogg T, Vasconcelos I. Volatile sulphur compounds in wines related to yeast metabolism and nitrogen composition of grape musts. Analytica Chimica Acta, 2002, 458: 157-167.

[24]Soufleros E H, Bouloumpasi E, Tsarchopoulos C, Biliaderis C G. Primary amino acid profiles of Greek white wines and their use in classification according to variety, origin and vintage. Food Chemistry, 2003, 80: 261-273.

[25]Taillandier P, Portugal F R, Fuster A, Strehaiano P. Effect of ammonium concentration on alcoholic fermentation kinetics by wine yeasts for high sugar content. Food Microbiology, 2007, 24: 95-100.

[26]Orlic S, Arroyo-López F N, Huic-Babic K, Lucilla I, Querol A, Barrio E. A comparative study of the wine fermentation performance of Saccharomyces paradoxus under different nitrogen concentrations and glucose/fructose ratios. Journal of Applied Microbiology, 2010, 108: 73-80.

[27]Garde-Cerdán T, Martínez-Gil A M, Lorenzo C, Lara J F, Pardo F, Salinas M R. Implications of nitrogen compounds during alcoholic fermentation from some grape varieties at different maturation stages and cultivation systems. Food Chemistry, 2011, 124: 106-116.

[28]Sablayrolles J M, Dubois C, Manginot C, Roustan J L, Barre P. Effectiveness of combined ammoniacal nitrogen and oxygen additions for completion of sluggish and stuck wine fermentations. Journal of Fermentation and Bioengineering, 1996, 82(4): 377-381.

[29]Hernández-orte P, Lapeña A C, Peña-Gallego A, Astrain J, Baron C, Pardo I, Polo L, Ferrer S, Cacho J, Ferreira V. Biogenic amine determination in wine fermented in oak barrels: Factors affecting formation. Food Research International, 2008, 41: 697-706.

[30]Uthurry C A, Suárez Lepe J A, Lombardero J, García Hierro J R. Ethyl carbamate production by selected yeasts and lactic acid bacteria in red wine. Food Chemistry, 2006, 94: 262-270.

[31]Vilanova M, Ugliano M, Varela C, Siebert T, Pretorius I S, Henschke P A. Assimilable nitrogen utilization and production of volatile and non-volatile compounds in chemically defined medium by Saccharomyces cerevisiae wine yeasts. Applied Microbiology and Biotechnology, 2007, 77: 145-157.

[32]Torrea D, Varela C, Ugliano M, Ancin-Azpilicueta C, Francis I L, Henschke P A. Comparison of inorganic and organic nitrogen supplementation of grape juice-Effect on volatile composition and aroma profile of a Chardonnay wine fermented with Saccharomyces cerevisiae yeast. Food Chemistry, 2011, 127: 1072-1083.

[33]张瑾，王国栋，惠竹梅. 模拟葡萄汁中添加可同化氮对酵母菌酒精发酵的影响. 西北农林科技大学学报，2010，38(3): 160-172.

Zhang J, Wang G D, Xi Z M. Influence of addition of assimilable nitrogen on alcoholic fermentation in chemically defined must by wine yeasts. Journal of Northwest A&F University, 2010, 38(3): 160-172. (in Chinese)

[34]张明霞，吴玉文，段长青. 葡萄与葡萄酒香气物质研究进展. 中国农业科学，2008, 41(7): 2098-2104.

Zhang M X, Wu Y W, Duan C Q. Progress in study of aromatic compounds in grape and wine. Scientia Agriculturae Sinica, 2008, 41(7): 2098-2104. (in Chinese)

[35]Moreira N, Mendes F, Guedes de Pinho P, Hogg T, Vasconcelos I. Heavy sulphur compounds, higher alcohols and esters production profile of Hanseniaspora uvarum and Hanseniaspora guilliermondii grown as pure and mixed cultures in grape must. International Journal of Food Microbiology, 2008, 124: 231-238.

[36]González-Marco A, Jiménez-Moreno N, Ancín-Azpilicueta C. Influence of nutrients addition to nonlimited-in-nitrogen must on wine volatile composition. Journal of Food Science, 2010, 75(4): 206-211.

[37]Losada M M, Andrés J, Cacho J, Revilla E, López J F. Influence of some prefermentative treatments on aroma composition and sensory evaluation of white Godello wines. Food Chemistry, 2011, 125: 884-891.

[38]Hernández-Orte P, Ibarz M J, Cacho J, Ferreira V. Effect of the addition of ammonium and amino acids to musts of Airen variety on aromatic composition and sensory properties of the obtained wine. Food Chemistry, 2005, 89: 163-174.

[39]Perestrelo R, Fernandes A, Albuquerque F F, Marques J C, Camara J S. Analytical characterization of the aroma of Tinta Negra Mole red wine: Identification of the main odorants compounds. Analytica Chimica Acta, 2006, 563: 154-164.

[40]Jiménez-Martí E, Aranda A, Mendes-Ferreira A, Mendes-Faia A, Olmo M I. The nature of the nitrogen source added to nitrogen depleted vinifications couducted by a Saccharomyces cerevisiae strain in synthetic must affects gene expression and the levels of several volatile compounds. Antonie van Leeuwenhoek, 2007, 92: 61-75.

[41]Lilly M, Bauer F F, Styger G, Lambrechts M G, Pretorius I S. The effect of increased branched-chain amino acid transaminase activity in yeast on the production of higher alcohols and on the flavour profiles of wine and distillates. FEMS Yeast Research, 2006, 6: 726-743.

[42]Sumby K M, Grbin P R, Jiranek V. Microbial modulation of aromatic esters in wine: Current knowledge and future prospects. Food Chemistry, 2010, 121: 1-16.

[43]Mendes-Ferreira A, Barbosa C, Iněs A, Mendes-Faia A. The timing of diammonium phosphate supplementation of wine must affects subsequent H2S release during fermentation. Journal of Applied Microbiology, 2010, 108: 540-549.

[44]Mendes-Ferreira A , Barbosa C, Falco V, Leāo C, Mendes-Faia A. The production of hydrogen sulphide and other aroma compounds by wine strains of Saccharomyces cerevisiae in synthetic media with different nitrogen concentrations. Journal of Industrial Microbiology and Biotechnology, 2009, 36: 571-583.

[45]Garde-Cerdan T, Ancín-Azpilicueta C. Effect of the addition of different quantities of amino acids to nitrogen-deficient must on the formation of esters, alcohols, and acids during wine alcoholic fermentation. Food Science and Technology, 2008, 41: 501-510.

[46]Carrau F M, Medina K, Farina L, Boido E, Henschke P A, Dellacassa E. Production of fermentation aroma compounds by Saccharomyces cerevisiae wine yeasts: effect of yeast assimilable nitrogen on two model strains. FEMS Yeast Research, 2008, 8: 1196-1207.

[47]Bely M, Rinaldi A, Dubourdieu D. Influence of assimilable nitrogen on volatile acidity production by Saccharomyces cerevisiae during high sugar fermentation. Journal of Bioscience and Bioengineering, 2003, 96(6): 507-512.

[48]Landaud S, Helinck S, Bonnarme P. Formation of volatile sulfur compounds and metabolism of methionine and other sulfur compounds in fermented food. Applied Microbiology and Biotechnology, 2008, 77: 1191-1205.

[49]Swiegers J H, Pretorius I S. Modulation of volatile sulfur compounds by wine yeast. Applied Microbiology and Biotechnology, 2007, 74: 954-960.

[50]马捷，刘延琳. 葡萄酒中重要挥发性硫化物的代谢及基因调控. 微生物学报，2011，51(1): 14-20.

Ma J, Liu Y L. Metabolism and gene regulation of important volatile sulfur compounds in wine-A review. Acta Microbiologica Sinica, 2011, 51(1): 14-20. (in Chinese)

[51]Jiranek V, Langridge P, Henschke P A. Regulation of hydrogen sulfide liberation in wine-producing Saccharomyces cerevisiae strains by assimilable nitrogen. Applied and Environmental Microbiology, 1995, 61(2): 461-467.

[52]Marks V D, Merwe G K, Vuuren H J J. Transcriptional profiling of wine yeast in fermenting grape juice: regulatory effect of diammonium phosphate. FEMS Yeast Research, 2003, 3: 269-287.

[53]Ugliano M, Kolouchova R, Henschke P A. Occurrence of hydrogen sulfide in wine and in fermentation: influence of yeast strain and supplementation of yeast available nitrogen. Journal of Industrial Microbiology and Biotechnology, 2011, 38: 423-429.

[54]Wang X D, Bohlscheid J C, Edwards C G. Fermentative activity and production of volatile compounds by Saccharomyces grown in synthetic grape juice media deficient in assimilable nitrogen and/or pantothenic acid. Journal of Applied Microbiology, 2003, 94: 349-359.

[55]Bohlscheid J C, Fellman J K, Wang X D, Ansen D, Edwards C G. The influence of nitrogen and biotin interactions on the performance of Saccharomyces in alcoholic fermentations. Journal of Applied Microbiology, 2007, 102: 390-400.

[56]Francis I L, Newton J L. Determining wine aroma from compositional data. Australian Journal of Grape and Wine Research, 2005, 11: 114-126.

[57]Swiegers J H, Pretorius I S. Yeast modulation of wine flavour. Advances in Applied Microbiology, 2005, 57: 131-175.

[58]Yoshimoto H, Fukushige T, Yonezawa T, Sone H. Genetic and physiological analysis of branched-chain alcohols and isoamyl acetate production in Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 2002, 59: 501-508.

[59]Laere Stijn D M, Sofie M G Saerens, Verstrepen K J, Dijck P, Thevelein J M, Delvaux F R. Flavour formation in fungi: characterization of KLAtf, the Kluyveromyces lactis orthologue of the Saccharomyces cerevisiae alcohol acetyltransferases Atf1 and Atf2. Applied Microbiology and Biotechnology, 2008, 78: 783-792.

[60]Hagen K M, Keller M, Edwards C G. Survey of biotin, pantothenic acid, and assimilable nitrogen in winegrapes from the Pacific Northwest. American Journal of Enology and Viticulture, 2008, 59(4): 432-436.

[61]Butzke C E. Survey of yeast assimilable nitrogen status in musts from California, Oregon, and Washington. American Journal of Enology and Viticulture, 1998, 49(2): 220-224.