关键词: 家蚕中肠')">家蚕中肠, 质型多角体病毒, 基因芯片, 应答基因, 实时荧光定量PCR

Abstract: 【Objective】The objective of this study is to screen the responsive gene of silkworm infected with Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) and establish a foundation for elaborating the molecular mechanism of BmCPV infection.【Method】A microarray system comprising about 23 000 oligonucluotide probes was employed to compare differentially expressed genes in the midguts of BmCPV-infected and normal silkworm larvae at 24, 48 and 72 h post-inoculation. Functions, classifications and pathways of these differentially expressed genes were analyzed by bioinformation tools. Seventeen differentially expressed genes were identified by quantitative real-time PCR. 【Result】At 24, 48 and 72 h post-inoculation, 9, 51 and 258 differentially expressed genes were obtained, respectively. KEGG pathways analysis indicated that at 48 and 72 h post-inoculation, most genes related to metabolism pathways were down-regulated while expressions of genes involved in ribosome and proteasome pathway were all up-regulated. The expressions of several immune-related genes including serpin5, lipase, heat shock proteins, cytochrome P450s and ribosomal P0 protein were up-regulated. 【Conclusion】Differentially expressed genes identified may be responsive to the BmCPV infection. The results provided new clues for investigating the molecular mechanism of BmCPV infection.

Key words: silkworm midgut')">silkworm midgut, Cytoplasmic polyhedrosis virus, gene chip, responsive gene, quantitative real-time PCR