水稻与稻瘟病菌不同小种互作的基因差异表达谱分析

doi:10.3864/j.issn.0578-1752.2013.24.006

中国农业科学 ›› 2013, Vol. 46 ›› Issue (24): 5123-5131.doi: 10.3864/j.issn.0578-1752.2013.24.006

水稻与稻瘟病菌不同小种互作的基因差异表达谱分析

姜兆远1, 2, 3, 任金平1, 2, 3, 刘晓梅1, 2, 3, 邹晓威1, 2, 3, 郭晓莉1, 2, 3, 王继春1, 2, 3, 温嘉伟2, 刘文平2, 夏海丰4, 洪德志5

1.吉林省农业科学院中国农业科技东北创新中心，长春130033；
2.吉林省农业科学院植物保护研究所，吉林公主岭136100；
3.农业部东北作物有害生物综合治理重点实验室，吉林公主岭136100；
4.吉林省通化市农业科学院，吉林通化135007；5吉林省农业技术推广总站，长春130021

收稿日期:2013-06-05 出版日期:2013-12-16 发布日期:2013-09-16
通讯作者: 任金平，Tel：0434-6283188；E-mail：15043461118@163.com
作者简介:姜兆远，Tel：0434-6283336；E-mail：jzy_80@163.com
基金资助:
吉林省科技厅重大专项（2060901）、吉林省博士后基金（01920）

Comparative Analysis of Gene Expression Profiles of Rice Responding to Different Races of Magnaporthe grisea

1.Northeast Agricultural Research Center of China, Jilin Academy of Agricultural Sciences, Changchun 130033;
2.Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin;
3.Key Laboratory of Integrated Pest Management on Crops in Northeast, Ministry of Agriculture, Gongzhuling136100, Jilin;
4.Tonghua Academy of Agricultural Sciences, Tonghua 135007, Jilin; 5Agricultural Technology and Extension Center of Jilin Province, Changchun 130021

Received:2013-06-05 Online:2013-12-16 Published:2013-09-16

摘要/Abstract

摘要： 【目的】分析水稻接种稻瘟病菌（Magnaporthe grisea）48 h后亲和互作与非亲和互作反应的基因表达谱，探索水稻对不同稻瘟病菌抗性差异的分子机理。【方法】运用Affymetrix表达谱芯片分析差异表达mRNA；通过分子注释系统平台（MAS 3.0）对差异表达基因进行了基因注释及GO分析；应用实时定量PCR 对部分差异表达基因进行验证。【结果】在49 824个转录本中共检测到大约24 000个转录本，Fold change大于2.5的基因共1 028个，其中非亲和互作上调基因460个，下调基因568个。所验证的4个基因的荧光定量PCR结果与芯片结果基本一致。经GO分析差异基因对应蛋白的功能主要涉及信号转导、酶的调节、转录及分子转运等。【结论】水稻与稻瘟病菌非亲和与亲和互作的基因表达谱存在较大差异，基因芯片筛选到的差异表达基因通过GO注释明确了差异基因的分子功能及信号通路，有利于进一步了解植物抗病机制，并可能为稻瘟病防治措施提供新的途径。

关键词: 稻瘟病 , 基因表达谱 , 基因芯片

Abstract: 【Objective】The objective of this study is to investigate the gene expression profiles of rice at 48 h after compatible and incompatible Magnaporthe grisea inoculation, and to explore the molecular mechanism of rice resistance to different M. griseas races.【Method】mRNA expression in compatible and incompatible rice was profiled using the Affymetrix microarray assay. GO (gene ontology) analyses were conducted on differentially expressed genes using a free web-based Molecular Annotation System 3.0 (MAS 3.0). Finally, the quantitative real-time PCR was used to verify the expression patterns of genes selected from microarray in rice. 【Result】Among the 49 824 transcripts on the array, about 24 000 transcripts were detected in rice. And 1 028 genes showed a fold change (FC) of more than 2.5. There were 460 genes were up-regulated and 568 genes were down-regulated in incompatible rice. Subsequently, real-time PCR was performed to validate four genes screened out by the microarray approach and sufficient consistency was observed between the two methods. The differentially expressed genes were functionally categorized by GO term analysis, which demonstrated that the gene set encoded proteins that function in signal transduction, enzyme regulation, transcription, molecule transport, and so on. 【Conclusion】 The result of gene expression profiles show that there were significant differences inthe compatible and incompatible rice. The genes differentially expressed in rice identified by microarray and functionally categorized by GO term analysis will help further understanding of plant disease resistance mechanisms in plant-pathogen interaction and may be beneficial to provide a new method for control of rice blast.

Key words: rice blast , gene expression profile , genechip

姜兆远1, 2, 3, 任金平1, 2, 3, 刘晓梅1, 2, 3, 邹晓威1, 2, 3, 郭晓莉1, 2, 3, 王继春1, 2, 3, 温嘉伟2, 刘文平2, 夏海丰4, 洪德志5. 水稻与稻瘟病菌不同小种互作的基因差异表达谱分析[J]. 中国农业科学, 2013, 46(24): 5123-5131.

JIANG Zhao-Yuan-1, 2 , 3 , REN Jin-Ping-1, 2 , 3 , LIU Xiao-Mei-1, 2 , 3 , ZOU Xiao-Wei-1, 2 , 3 , GUO Xiao-Li-1, 2 , 3 , WANG Ji-Chun-1, 2 , 3 , WEN Jia-Wei-2, LIU Wen-Ping-2, XIA Hai-Feng-4, HONG De-Zhi-5. Comparative Analysis of Gene Expression Profiles of Rice Responding to Different Races of Magnaporthe grisea[J]. Scientia Agricultura Sinica, 2013, 46(24): 5123-5131.

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水稻与稻瘟病菌不同小种互作的基因差异表达谱分析

Comparative Analysis of Gene Expression Profiles of Rice Responding to Different Races of Magnaporthe grisea

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