基于蔗糖代谢基因多态性的甘蔗基因型遗传多样性

doi:10.3864/j.issn.0578-1752.2011.09.004

摘要/Abstract

摘要： 【目的】探讨利用基于蔗糖代谢酶基因多态性的目标区域扩增多态性（target region amplification polymorphism, TRAP）标记进行甘蔗遗传多样性、遗传距离及遗传距离与糖分表型值关联性分析的可行性。【方法】利用4个根据参与蔗糖代谢酶基因SuSy、SPS、SAI 和PPDK设计的锚定引物和9个随机引物, 筛选具有多态性的17对引物组合, 对28个糖分性状表型不同的甘蔗基因型进行TRAP标记, 通过分子标记数据，估算不同基因型蔗糖代谢基因的遗传变异和遗传距离, 并进行聚类分析。【结果】共扩增出170个条带, 其中109个为多态性条带, 多态性比率为64.1 %, 平均每个引物组合产生10个条带, 6.4个多态性条带。在所测试的28个甘蔗基因型中, 基于蔗糖代谢酶基因多态性所估计的遗传相异系数（genetic dissimilarity, GD）为0.12—0.80。基于GD的UPGMA聚类结果显示, 在GD=0.49处，供试基因型可被划分为4类，但就所采集的特定时期的锤度数据而言，类间和类内的锤度表型值没有明显的规律。【结论】甘蔗不同基因型间4个蔗糖代谢酶基因遗传变异较高, 多态性丰富, 暗示TRAP技术在评价甘蔗蔗糖分性状方面具有应用潜力, 但与锤度表型值数据的关联性还有待进一步研究。

关键词: 甘蔗 , 蔗糖代谢基因 , 目标区域扩增多态性（TRAP）标记 , 遗传多样性

Abstract: 【Objective】 The research objective is to evaluate the feasibility of target region amplification polymorphism (TRAP) analysis on sugarcane genetic diversity, genetic distance and the association between the genetic distance and phenotype value of sucrose content, which was based on the polymorphism of sucrose metabolism genes. 【Method】 Four fixed primers derived from candidate genes of SuSy, SPS, SAI and PPDK involved in sucrose metabolism were used in combination with nine arbitrary primers. Genetic dissimilarity (GD) was estimated by TRAP markers with 17 primer combinations among 28 sugarcane genotypes. The genetic variability and genetic distance between different genotypes were estimated based on the molecular marker data and the cluster analysis was conducted. 【Result】 A total of 170 fragments were scored and among them 64.1% i.e.109 fragments were polymorphic. The average fragments and polymorphic fragments yielded by each primer combination tested were 10 and 6.4, respectively. The GD based on polymorphism in sucrose metabolism genes estimated among a set of 28 genotypes ranged from 0.12 to 0.80. The clustering of genotypes, based on the GD of sucrose metabolism genes tested at the level of 0.49, showed four groups was obtained. Not obvious regular distribution being found between groups or within the group when referring to the Brix value in specific period in the experiment. 【Conclusion】 The results suggested that genetic diversity in the four evaluated sucrose genes was higher and the marker was rich in polymorphism. It suggested a high potential application of TRAP technique in the evaluation of sucrose content trait in sugarcane, while its relevance to the data of phenotypic brix need to be further studied.

Key words: sugarcane , sucrose metabolism genes , TRAP marker , genetic diversity

许莉萍，阙友雄，苏亚春. 基于蔗糖代谢基因多态性的甘蔗基因型遗传多样性[J]. 中国农业科学, 2011, 44(9): 1788-1797.

XU Li-ping, QUE You-xiong, SU Ya-chun. Genetic Diversity Among Sugarcane Genotypes Based on Polymorphisms in Sucrose Metabolism Genes[J]. Scientia Agricultura Sinica, 2011, 44(9): 1788-1797.

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