关键词: 巴什拜羊, 微卫星标记, 遗传多样性, 生长指标, 关联性

Abstract:

【Objective】 Taking Bashbay sheep as research material, this paper aimed at seeking the microsatellite marker which influenced the growth property of Bashbay sheep through analysis on the genotype of microsatellite DNA locus and growth property, and thus providing a basis for the molecular marker assisted selection. 【Method】 Inquiried about the microsatellite locus of sheep and goat from GenBank database, 10 microsatellite markers that meet the requirements of the microsatellite standard were selected, 189 individual genome DNA of sheep were detected, and the correlated effects of microsatellite locus and growth indexes were analyzed by adopting least-squares method. 【Result】 There were 110 allelic genes were detected by 10 microsatellite markers, with an average of 11 allelic genes for each, the average polymorphic information content was 0.7914, the average heterozygosity was 0.8149. There were 8 of 10 microsatellite locus had significant and very significant relation with 5 growth indexes including weight and size of Bashbay sheep, especially the significant correlation of gene locus BM415 and weight and size (P＜0.01). Multiple comparative results showed that 8 microsatellite locus had genotype advantage.【Conclusion】 Significant effect was found in the growh indexes of Bashbay sheep group, and the higher microsatellite locus offered valuable genetic markers for developing the molecular marker assisted breeding of growth indexes of Bashbay sheep.

Key words: Bashbay sheep, microsatellite markers, genetic diversity, growth indexes, association