桔小实蝇,微卫星标记,遗传多样性,遗传分化," /> 桔小实蝇,微卫星标记,遗传多样性,遗传分化,"/> Bactrocera dorsalis (Hendel),microsatellite marker,genetic diversity,genetic differentiation,"/> <font face="Verdana">基于微卫星分子标记的重庆地区桔小实蝇遗传分化研究</font>

中国农业科学 ›› 2010, Vol. 43 ›› Issue (13): 2688-2696 .doi: 10.3864/j.issn.0578-1752.2010.13.008

• 植物保护 • 上一篇    下一篇

基于微卫星分子标记的重庆地区桔小实蝇遗传分化研究

万宣伍,刘映红,张彬,周浩东   

  1. (西南大学植物保护学院/重庆市昆虫学及害虫控制工程重点实验室)
  • 收稿日期:2009-12-28 修回日期:2010-04-09 出版日期:2010-07-01 发布日期:2010-07-01
  • 通讯作者: 刘映红

Genetic Differentiation Among Poupulations of the Oriental Fruit Fly Bactrocera dorsalis (Hendel) in Chongqing Based on Microsatellite Markers #br#

WAN Xuan-wu, LIU Ying-hong, ZHANG Bin, ZHOU Hao-dong#br#   

  1. (西南大学植物保护学院/重庆市昆虫学及害虫控制工程重点实验室)
  • Received:2009-12-28 Revised:2010-04-09 Online:2010-07-01 Published:2010-07-01
  • Contact: LIU Ying-hong

摘要:

【目的】探讨重庆地区桔小实蝇遗传分化情况。【方法】以8对微卫星引物对重庆地区6个桔小实蝇种群155个个体的遗传多样性进行研究。【结果】在8个微卫星位点上共检测到51个等位基因。6个种群在各位点的平均表观杂合度Ho为0.1731—0.2958,平均期望杂合度He为0.1311—0.6796,经卡方检验,各种群在大多数位点上不符合Hardy-Weinberg平衡。对多态位点百分率P、基因多样性指数Nei’s,Shannon信息指数I的分析表明,本文所研究的6个桔小实蝇种群具有较丰富的遗传多样性。种群间分化系数FST平均值为0.0777,说明遗传变异主要发生在种群内。武隆种群和永川种群之间的遗传距离最大(0.2367),江津种群和北碚种群之间的遗传距离最小(0.0667)。UPGMA聚类分析显示,重庆地区桔小实蝇大致分为两个亚群,北碚、江津、万州、秀山、永川5个种群为一个亚群,武隆种群单独为一个亚群。【结论】重庆地区的桔小实蝇出现了一定的遗传分化,但分化程度不高。桔小实蝇在重庆地区的入侵处于初级阶段。

关键词: 桔小实蝇')">桔小实蝇, 微卫星标记, 遗传多样性, 遗传分化

Abstract:

【Objective】 The objective of the study is to discuss the genetic differentiation of the Bactrocera dorsalis (Hendel) in Chongqing region. 【Method】 One hundred and fifty-five individuals of B.dorsalis collected from 6 districts of Chongqing were divided into 6 populations and analyzed by 8 microsatellite markers to examine the genetic diversity and genetic differentiation in Chongqing region. 【Result】 A total of 51 alleles were detected at 8 loci. The range of Mean Observed Heterozygote (Ho) was from 0.1731 to 0.2958 and the range of Mean Expected Heterozygote (He) was from 0.1311 to 0.6796. The Chi-square test results argued that the 6 populations of B. dorsalis in present study did not follow with the Hardy-Weinberg Equilibrium at most loci. The analysis results of Percentage of Polymorphic Loci, Nei’s Gene Diversity and Shannon Information Index indicated that there was a high level in genetic diversity among the 6 populations of B. dorsalis. The mean Genetic Differentiation Index (FST) was 0.0777, which demonstrated that genetic differentiation mainly occurred inner-population. The utmost genetic distance (0.2367) appeared between Wulong population and Yongchuan population, meanwhile the nearest genetic distance (0.0667) occurred between Jiangjin population and Beibei population. The UPGMA phylogenetic tree showed that the 6 populations in Chongqing region clustered into two groups. Populations of Beibei, Jiangjin, Wanzhou, Xiushan, and Yongchuan merged into one group, while population from Wulong solely formed the other group.【Conclusion】 The results suggested that genetic differentiation already occurred among the 6 populations, although the degree of differentiation was low. The invasion of the oriental fruit fly in Chongqing region was at the primary stage.

Key words: Bactrocera dorsalis (Hendel)')">Bactrocera dorsalis (Hendel), microsatellite marker, genetic diversity, genetic differentiation