关键词: 籼稻, 地方品种, 微卫星标记, 遗传多样性, 指纹图谱

Abstract:

【Objective】 The genetic diversity of indica rice landraces from different provinces of China was analysed to provide a genetic basis for construction of fingerprinting of indica rice landraces from China and its effective protection and utilization.【Method】 The fingerprinting map of 280 indica rice landraces from 14 provinces of China was constructed using 78 microsatellite markers distributing over the whole rice genome. The difference of genetic diversity, the composition and frequency of alleles of SSR markers for indica rice landraces from different provinces were analysed. 【Result】 The results indicated that a total of 330 alleles were detected, and the number of alleles per pair of marker were ranged from 2 to 12 with the average alleles of 4.141. The number of alleles and effective number of alleles for RM336, RM334, RM264, RM297, RM204, RM248 and RM444 markers were more than 6 and 3, respectively, which showed higher genetic diversity indexes, were more than 1.4. The frequency of alleles detected by 78 SSR primers was ranged from 0.18% to 99.56%, and the A allele of RM338 showed the highest frequency, the E allele of RM131 and the F allele of RM276 showed the lowest frequency. Some alleles of 44 SSR primers such as RM338, RM308, RM484 and RM29, showed 100% frequency of appearance in indica rice landraces in most of the provinces. RM336, RM334, RM264, RM204, RM297, RM248, RM263, RM276, RM444, RM21, RM246 and RM258 showed higher genetic diversity in most of the provinces. 【Conclusion】 The above 12 pairs of primers are suitable for the fingerprinting construction of indica rice landraces in China.

Key words: indica rice, landrace, microsatellite marker, genetic diversity, fingerprinting