关键词: 泰拉霉素, 红霉素, 一氧化氮, 前列腺素E2, 诱导型一氧化氮合成酶, 环氧合酶-2

Abstract:

【Objective】 The anti-inflammatory effects of tulathromycin were studied. 【Method】 In vitro, we make use of the model that is LPS-induced porcine peripheral blood mononuclear cells (PBMC) overexpressing pro-inflammation factors was used, and then the real-time Q-PCR was employed to study the effect of different concentrations of tulathromycin and erythromycin on the generation of NO and PGE2 involved in the inflammatory process on the molecular level. 【Result】 Results showed that tulathromycin at the concentrations of 5, 10 and 20 μg•mL-1 significantly decreased the production of NO and PGE2, a similar pattern was also observed on the cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS) gene transcription. However, all 5, 10 and 20 μg•mL-1 of erythromycin significantly decreased the production of NO and PGE2 on the transcription and expression level. 【Conclusion】 These results support the opinion that macrolides may exert anti-inflammatory effect through modulating the synthesis of NO and PGE2 in inflammatory process.

Key words: tulathromycin, erythromycin, NO, PGE2, iNOS, COX-2