Abstract:

【Objective】Intercellular washing fluid (IWF) obtained from wheat leaves infected by leaf rust contains specific elicitors that was used to stimulate the suspension cell of wheat (cv. Lovrin 10) in order to study the nitric oxide (NO) acting and its possible molecular mechanism.【Method】Greiss reagent and fluorescent NO probe DAF-2DA were used to detecting of dynamic changes of intracellular NO. Pharmacological experiments were carried out to study the signal interactions between calcium (Ca2+) and NO.【Result】IWF-260 could cause the production of NO in wheat suspension cultured cells. It played an important role in the programmed death of wheat suspension cells which was induced by IWF-260. Pharmacological inhibitor experiments proved that NOS and NR might be involved in the production of NO, and the influx of extracellular Ca2 + could regulate the production of NO. NR pathway may be the major route in NO production. 【Conclusion】IWF-260 can induce the production of NO in wheat suspension cells, and this process is involved with the NOS, NR and the influx of extracellular Ca2+.