关键词: 捻转血矛线虫, 半乳糖结合凝集素, 细胞因子, 山羊

Abstract:

【Objective】 Cytokines in abomasum of goats immunized with rHco-GAL-f/m were detected in this study. 【Method】 In situ hybridization was used to localize IL-4, IL-6, IL-1?, IL-2,IFN-γ and TNF-α mRNAs in abomasum of the negative control group (A), challenged control group (B), vaccinated-challenged group (C) and vaccinated-unchallenged group (D) goats immunized with rHco-GAL-f/m, and the numbers of cytokine mRNA+ cells in each group were also analyzed. 【Result】 No cytokines were found in the abomasum of group A goats and IL-4, IL-6, IL-1?, IL-2, IFN-γ and TNF-α were detected in the submucosa layer of abomasum of groups B, C and D goats. The difference of IL-4 and IL-6 mRNA+ cell numbers in groups B, C and D was significant (P＜0.05) and group C was higher than that of groups B and D. The difference of IL-1?, IL-2 and, TNF-α mRNA+ cell numbers was also significant (P＜0.05) among groups B,C and D, and group D was the highest. IFN-γ mRNA+ cells were not significantly different between groups B and C (P＞0.05), but IFN-γ mRNA+ cells in group D was significantly different and was higher compared to groups B and C (P＜0.05). 【Conclusion】 The findings indicated that the recombinant galectins of H. contortus are capable of inducing local immune responses in abomasum of goats.

Key words: Haemonchus contoruts, galectin, cytokines, goat