关键词: 白菜（Brassica campestris L. ssp. chinensis Makino）, 雄性不育, 基因克隆, BcMF1基因, 特征分析

Abstract: Abstract: 【Objective】This paper aimed to screening a novel gene related with the male sterility in Chinese cabbage for studing the molecular mechanisim of plant male sterility. 【Method】The gene differential expression analysis was performed by cDNA-AFLP in the genic male sterile-fertile line ZUBajh97-01AB of Chinese cabbage, and a differentially expressed cDNA fragment, BcMF-A15T17, was found in fertile line. The full cDNA and DNA sequences of the gene related with BcMF-A15T17 were amplified by RACE and PCR, and the expression characteristic of the gene was proved by Northern blot analysis. 【Result】 The gene, designated as BcMF1, was 1684 bp long in cDNA and1985 bp long in DNA, and specially expressed in middle and big floral buds of the fertile line. The hypothetical protein BcMF1 including 471 amino acids , which was predicted being a extracellular protein in Secretory pathway, had higher similarity in the amino acids sequence to some members from a unknown protein family DUF1216 in Arabidopsis thaliana.【Conclusion】 All the results in this study suggest that BcMF1 is a new gene related with genic male sterility in Chinese cabbage.