Volume 23, Issue 03, Mar. 2024
We initially identified differentially expressed genes (DEGs) by comparing the transcriptomes of folic acid (FA)-treated and water-treated (CK) berries at different time points, and then analyzed the sequences to detect alternatively spliced (AS) genes associated with postharvest softening. During the postharvest storage of FA-treated berries, the VvPE2 gene, which encodes a cell wall-degrading enzyme, undergoes alternative splicing giving rise to two transcripts: the full-length isoform VvPE2.1 and the alternatively spliced form VvPE2.2. VvPE2.1 possesses the complete structure of the PECTINESTERASE gene, and the encoded protein maintains full enzyme functionality, whereas VvPE2.2 encodes an incomplete protein. The up-regulation of VvPE2.2 in FA-treated berries is expected to reduce the accumulation of VvPE2.1, subsequently leading to reduced enzymatic degradation of pectin by PE, thus delaying berry softening. This study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries. The cover photo was provided by Dr. Maosong Pei, College of Horticulture and Plant Protection, Henan University of Science and Technology. See pages 863–875 for details.
Volume 23, Issue 02, Feb. 2024
Schrankia costaestrigalis is a newly-reported potato pest in southern China. The larvae damage potato tubers by feeding on tubers and foliage, either in the field or during storage. The pest has caused great economic losses to farmers in the winter and early-spring potato planting areas. Early-warning monitoring of this pest could protect domestic potato production. Based on the environmental data (temperature and precipitation variables) and human influence index, the optimal species distribution model was used to estimate the potential geographical distribution in China under climate change. Results showed that highly suitable habitats of S. ostaestrigalis were mainly located in Guangxi, Guangdong, Hong Kong, Fujian and Hainan. The potential geographical distribution of S. ostaestrigalis in China will decrease under climate change, which shows a general tendency to move northeast and to the middle-high latitudes in the 2030s. The agricultural practice of plastic film mulching in potato fields will provide a favorable microclimate for S. ostaestrigalis in the suitable areas. More attention should be paid to the early warning and monitoring of S. ostaestrigalis in order to prevent its further spread in the China’s winter potato planting regions. The cover photo depicts the developmental stages of S. ostaestrigalis, which is provided by Dr. Xian Xiaoqing and Ph D student Zhao Haoxiang from Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing. See pages 2441–2455 for details.