Molecular mapping of stripe rust resistance gene YrH9017 in wheat-Psathyrostachyshuashanica introgression line H9017-14-16-5-3
MA Dong-fang1, 2*, HOU Lu1*, SUN Cai2*, ZHANG Xing2, YIN Jun-liang2, GUO Qing-yun1, ZHU Yong-xing2
1 Qinghai Academy of Agriculture and Forestry Sciences, Qinghai University/Key Laboratory of Agricultural Integrated Pest Management, Qinghai Province/State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, P.R.China 2 Hubei Collaborative Innocvtion Center for Grain Industry/College of Agriculture, Yangtze University, Jingzhou 434023, P.R.China
Several new stripe rust pathogen races emerged in the wheat growing regions of China in recent years. These races were virulent to most of the designated wheat seedling resistance genes. Thus, it is necessary and worthwhile to identify new valuable resistant materials for the sake of diversifying resistant sources, pyramiding different resistance genes and achieving durable resistance. Here, we identified the resistance gene, temporarily designated as YrH9017, in wheat-Psathyrostachyshuashanica introgression line H9017-14-16-5-3. A total of 146 F2 plants and their derived F2:3 families in a cross of Mingxian 169
and H9017-14-16-5-3 were used to evaluate seedling stripe rust response and as a mapping population. Finally, we constructed a genetic map including eight simple sequence repeat (SSR) markers and expressed sequence tag (EST) markers. YrH9017 was located on the long arm of chromosome 2A and closely linked with two EST-sequence tagged site (EST-STS) markers BG604577 and BE471201 at 1.3 and 1.8 cM distance, respectively. The two closest markers could be used for marker-assisted selection of YrH9017 in breeding.
Fund: The research was funded by the National Natural Science Foundation of China (31660513, 31501620 and 31701911)and the Provincial Natural Science Foundation of Qinghai, China (2017-ZJ-793). Finally, we thank Prof. Qiao Yongli (Shanghai Normal University, China) for beneficial comments on the initial project design and data analysis.
Corresponding Authors: Correspondence GUO Qing-yun, Tel: +86-971-5311056, E-mail: firstname.lastname@example.org; ZHU Yong-xing, Tel: +86-716-8066302, E-mail: email@example.com
* These authors contributed equally to this study.
About author: MA Dong-fang, E-mail: firstname.lastname@example.org; HOU Lu, E-mail: email@example.com; SUN Cai, E-mail: SUNCAI1211 @163.com;
Cite this article:
MA Dong-fang, HOU Lu, SUN Cai, ZHANG Xing, YIN Jun-liang, GUO Qing-yun, ZHU Yong-xing. 2019. Molecular mapping of stripe rust resistance gene YrH9017 in wheat-Psathyrostachyshuashanica introgression line H9017-14-16-5-3. Journal of Integrative Agriculture,