Effects of Bupleurum extract on blood metabolism, antioxidant status and immune function in heat-stressed dairy cows
CHENG Jian-bo1*, FAN Cai-yun1*, SUN Xian-zhi1, WANG Jia-qi2, 3, ZHENG Nan2, 3, ZHANG Xing-kai4, QIN Jun-jie5, WANG Xiu-min5
1 College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China 2 Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China 3 State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China 4 Shanghai Bright Holstan Co., Ltd., Shanghai 200436, P.R.China 5 Beijing Centre Biology Co., Ltd., Beijing 102206, P.R.China
Abstract This experiment was conducted to evaluate the effects of Bupleurum extract (BE) on blood metabolites, antioxidant status, and immune function in dairy cows under heat stress. Forty lactating Holstein cows were randomly assigned to 1 of 4 treatments. The treatments consisted of 0, 0.25, 0.5, and 1.0 g of BE kg–1 dry matter. Supplementation with BE decreased (P<0.05) blood urea nitrogen (BUN) contents and increased blood total protein (TP) and albumin (ALB) levels compared with control cows, but it had no effects (P>0.05) on blood glucose (GLU), nonesterified fatty acid (NEFA), total triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high density lipoprotein cholesterol (HDL-C). Compared with control cows, cows fed BE had higher (P<0.05) superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity. However, supplementation with BE had no effect (P>0.05) on total antioxidant capacity (T-AOC) or malondialdehyde (MDA) levels. The immunoglobulin (Ig) A and G contents increased (P<0.05) in cows fed 0.25 or 0.5 g of BE kg–1. Interleukin (IL)-2 and IL-4 levels were higher (P<0.05) in cows fed 0.5 and 1.0 g of BE kg–1, and IL-6 was significantly elevated (P<0.05) in cows fed 0.5 g of BE kg–1. There were no treatment effects (P>0.05) on the CD4+ and CD8+ T lymphocyte ratios, CD4+/CD8+ ratio, or tumor necrosis factor-α (TNF-α) level among the groups. These findings suggest that BE supplementation may improve protein metabolism, in addition to enhancing antioxidant activity and immune function in heat-stressed dairy cows.
This study was supported financially by the National Key Research and Development Program of China (2016YFD0500503), the Agricultural Science and Technology Innovation Program, China (ASTIP-IAS12) and the Anhui International Science and Technology Cooperation plan program, China (1503062019).
About author: CHENG Jian-bo, E-mail: email@example.com;
* These authors contributed equally to this study.
Cite this article:
CHENG Jian-bo, FAN Cai-yun, SUN Xian-zhi, WANG Jia-qi, ZHENG Nan, ZHANG Xing-kai, QIN Junjie, WANG Xiu-min. Effects of Bupleurum extract on blood metabolism, antioxidant status and immune function in heat-stressed dairy cows[J]. Journal of Integrative Agriculture,
2018, 17(03): 657-663.
Abeni F, Calamari L, Stefanini L. 2007. Metabolic conditions of lactating Friesian cows during the hot season in the Po valley. 1. Blood indicators of heat stress. International Journal of Biometeorology, 52, 87-96.
Armstrong D V. 1994. Heat stress interaction with shade and cooling. Journal of Dairy Science, 77, 2044-2050.
Ashour M L, Wink M. 2011. Genus Bupleurum: A review of its phytochemistry, pharmacology and modes of action. Journal of Pharmacy and Pharmacology, 63, 305-321.
Bernabucci U, Ronchi B, Lacetera N, Nardone A. 2002. Markers of oxidative status in plasma and erythrocytes of transition dairy cows during hot season. Journal of Dairy Science, 85, 2173-2179.
Caraher E M, Parenteau M, Gruber H, Scott F W. 2000. Flow cytometric analysis of intracellular IFN-γ, IL-4 and IL-10 in CD3+4+ T-cells from rat spleen. Journal of Immunological Methods, 244, 29-40.
Caroprese M, Marzano A, Entrican G, Wattegedera S, Albenzio M, Sevi A. 2009. Immune response of cows fed polyunsaturated fatty acids under high ambient temperatures. Journal of Dairy Science, 96, 2796-2803.
Carroll J A, Burdick N C, Chase J C C, Coleman S W, Spiers D E. 2012. Influence of environmental temperature on the physiological, endocrine, and immune responses in livestock exposed to aprovocative immune challenge. Domestic Animal Endocrinology, 43, 146-153.
Collier R J, Dahl G E, VanBaale M J. 2006. Major advances associated with environmental effects on dairy cattle. Journal of Dairy Science, 89, 1244-1253.
Guo Y J, Matsumoto T, Kikuchi Y J, Ikejima T, Wang B X, Yamada H. 2000. Effects of a pectic polysaccharide from a medicinal herb, the roots of Bupleurum falcatum L. on interleukin 6 production of murine B cells and B cell lines. Immunopharmacology, 49, 307-316.
Hammami H, Bormann J, M’hamdi N, Montaldo H H, Gengler N. 2012. Evaluation of heat stress effects on production traits and somatic cell score of Holsteins in a temperate environment. Journal of Dairy Science, 96, 1-12.
Harmon R J, Lu M, Trammel D S, Smith B A. 1997. Influence of heat stress and calving on antioxidant activity in bovine blood. Journal of Dairy Science, 80(Suppl. 1), 264.
Huang K. 1993. Antipyretic herbs. In: The Pharmacology of Chinese Herbs. CRC Press, Tokyo. pp. 151-152.
Kamiya M, Kamiya Y, Tanaka M, Oki T, Nishiba Y, Shioya S. 2006. Effects of high ambient temperature and restricted feed intake on urinary and plasma 3-methylhistidine in lactating Holstein cows. Animal Science Journal, 77, 201-207.
Kang H, Choib T W, Ahnb K S, Leec J Y, Hamc I H, Choic H Y, Shim E S, Sohn N W. 2009. Upregulation of interferon-γ and interleukin-4, Th cell-derived cytokines by So-Shi-Ho-Tang (Sho-Saiko-To) occurs at the level of antigen presenting cells, but not CD4+ T cells. Journal of Ethnopharmacology, 123, 6-14.
Lin H, Decuypere E, Buyse J. 2006. Acute heat stress induces oxidative stress in broiler chickens. Comparative Biochemistry and Physiology (Part A), 144, 11-17.
Liu J J, Xue H, Chen Z F. 1997. The effects of water soluble substance extracted from Xiao Chai-hu-tang on IL-2 production and CD antigen change of PHA-stimulated PBMC. Journal of Luzhou Medical College, 20, 331-334. (in Chinese)
Malek T R. 2003. The main function of IL-2 is to promote the development of T regulatory cells. Journal of Leukocyte Biology, 74, 961-965.
Martin S, Padilla E, Ocete M A, Galvez J, Jiménez J, Zarzuelo A. 1993. Anti-inflammatory activity of the essential oil of Bupleurum fruticescens. Planta Medica, 59, 533-536.
Matsumoto T, Cyong J C, Kiyohara H, Matsui H, Abe A, Hirano M, Danbara H, Yamada H. 1993. The pectic polysaccharides from Bupleurum falcatum L. enhances immune-complexes binding to peritoneal macrophages through Fc receptor expression. International Journal of Immunopharmacology, 15, 683-693.
Matsumoto T, Noguchi M, Hayashi O, Makino K, Yamada H. 2010. Hochuekkito, a kampo (traditional Japanese herbal) medicine, enhances mucosal IgA antibody response in mice immunized with antigen-entrapped biodegradable microparticles. Evidence-Based Comple and Alternative Medicine, 7, 69-77.
Navarro P, Giner R M, Recio M C, Máñez S, Cerdá-Nicolás M, Ríos J L. 2001. In vivo anti-inflammatory activity of saponins from Bupleurum rotundifolium. Life Science, 68, 1199-1206.
NRC (National Research Council). 2001. Nutrient Requirements of Dairy Cattle. 7th ed. National Academy Press, Washington, D.C.
Ohtake N, Yamamoto M, Takeda S C, Aburada M, Ishige A, Watanabe K, Inoue M. 2005. The herbal medicine Sho-saiko-to selectively inhibits CD8+ T-cell proliferation. European Journal of Pharmacology, 507, 301-310.
Pan L, Bu D P, Wang J Q, Cheng J B, Sun X Z, Zhou L Y, Qin J J, Zhang X K, Yuan Y M. 2014. Effects of Radix Bupleuri extract supplementation on lactation performance and rumen fermentation in heat-stressed lactating Holstein cows. Animal Feed Science and Technology, 187, 1-8.
Park Y H, Fox L K, Hamilton M J, Davis W C. 1993. Suppression of proliferative response of BoCD4+ T lymphocytes by activated BoCD8+ T lymphocytes in the mammary gland of cows with Staphylococcus aureus mastitis. Veterinary Immunology and Immunopathology, 36, 137-151.
Sakurai M H, Matsumoto T, Kiyohara H, Yamada H. 1999. B cell proliferation activity of pectic polysaccharide from amedicinal herb, the roots of Bupleurum falcatum L. and its structural requirement. Immunology, 97, 540-547.
SAS (Statistical Analysis System). 2008. User’s Guide: Statistics. Version 9.2 edition. SAS Institute, Cary, NC.
St-Pierre N R, Cobanov B, Schnitkey G. 2003. Economic losses from heat stress by stressby US livestock industries. Journal of Dairy Science, 86(Suppl.), E52-E77.
Sun H X. 2006. Haemolytic activities and adjuvant effect of Bupleurum chinense saponins on the immune responses to ovalbumin in mice. Vaccine, 24, 1324-1331.
Sun L W, Feng K, Jiang R, Chen J Q, Zhao Y, Ma R, Tong H B. 2010. Water-soluble polysaccharide from Bupleurum chinense DC: Isolation, structural features and antioxidant activity. Carbohydrate Polymers, 79, 180-183.
Sun X B, Matsumoto T, Yamada H. 1991. Effects of a polysaccharide fraction from the roots of Bupleurum falcatum L. on experimental gastric ulcer models in rats and mice. Journal of Pharmacy and Pharmacology, 43, 699-704.
Wang B J, Liu C T, Tseng C Y, Yu Z R. 2005. Antioxidant activity of Bupleurum kaoi Liu (Chao et Chuang) fractions fractionated by supercritical CO2. LWT-Food Science and Technology, 38, 281-287.
Wang J P, Bu D P, Wang J Q, Huo X K, Guo T J, Wei H Y, Zhou LY, Rastani R R, Baumgard L H, Li F D. 2010. Effect of saturated fatty acid supplemen-tation on production and metabolism indices in heat-stressed mid-lactation dairy cows. Journal of Dairy Science, 93, 4121-4127.
Wheelock J B, Rhoads R P, VanBaale M J, Sanders S R, Baumgard L H. 2010. Effects of heat stress on energetic metabolism in lactating Holstein cows. Journal of Dairy Science, 93, 644-655.
Yamaguchi N, Kohno H, Tawara M, Odashima S. 1985. Effect of saikosaponin derivatives upon the immune response against T-dependent and T-independent antigens in mice. International Journal of Immunopharmacology, 7, 827-832.
Yen M H, Lin C C, Yen C M. 1995. The immunomodulatory effect of saikosaponin derivatives and the root extract of Bupleurumkaoi in mice. Phytotherapy Research, 9, 351-358.
Zhao W, Li J J, Yue S Q, Zhang L Y, Dou K F. 2012. Antioxidant activity and hepatoprotective effect of a polysaccharide from Bei Chaihu (Bupleurum chinense DC). Carbohydrate Polymers, 89, 448-452.
Zimbelman R B, Collier R J, Bilby T R. 2013. Effects of utilizing rumen protected niacin on core body temperature as well as milk production and composition in lactating dairy cows during heat stress. Animal Feed Science and Technology, 180, 26-33.