Development of elite restoring lines by integrating blast resistance and low amylose content using MAS
XIAO Wu-ming1*, PENG Xin1*, LUO Li-xin1, LIANG Ke-qin1, WANG Jia-feng1, HUANG Ming1, LIU Yong-zhu1, GUO Tao1, LUO Wen-long1, YANG Qi-yun2, ZHU Xiao-yuan2, WANG Hui1, CHEN Zhi-qiang1
1 National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, P.R.China 2 Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, P.R.China
Abstract Blast resistance and grain quality are major problems in hybrid rice production in China. In this study, two resistance (R) genes, Pi46 and Pita, along with the gene Wxb, which mainly affects rice endosperm amylose content (AC), were introgressed into an elite indica restoring line, R8166, which has little blast resistance and poor grain quality through marker-assisted selection (MAS). Eight improved lines were found to have recurrent genome recovery ratios ranging from 88.68 to 96.23%. Two improved lines, R163 and R167, were selected for subsequent studies. R167, which has the highest recovery ratio (96.23%), showed no significant differences in multiple agronomic traits. In contrast, R163 with the lowest recovery ratio (88.68%) exhibited significant differences in heading date and yield per plant compared with the recurrent parent. At two developmental stages, R163 and R167 had greatly enhanced resistance to blast over the recurrent parent. Similar trends were also observed for agronomic traits and blast resistance in R163- and R167-derived hybrids when compared with the counterparts from R8166. In addition, R163, R167, and their derived hybrids significantly improved the grain quality traits, including amylose content (AC), gel consistency (GC), chalky grain rate (CGR), and degree of endosperm chalkiness (DEC). It confirmed the success of efficiently developing elite restoring lines using MAS in this study.
This research was supported by the grant from the State Scholarship Fund of China (20153069), the the National Key R&D Program of China (2016YFD0101100) and by the earmarked fund for China Agriculture Research System (CARS-01-12).
About author: XIAO Wu-ming, Mobile: +86-15914508647, E-mail: heredity24 @126.com;* These authors contributed equally to this study.
Cite this article:
XIAO Wu-ming, PENG Xin, LUO Li-xin, LIANG Ke-qin, WANG Jia-feng, HUANG Ming, LIU Yong-zhu, GUO Tao, LUO Wen-long, YANG Qi-yun, ZHU Xiao-yuan, WANG Hui, CHEN Zhi-qiang. Development of elite restoring lines by integrating blast resistance and low amylose content using MAS[J]. Journal of Integrative Agriculture,
2018, 17(01): 16-27.
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