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园艺作物品质提升与逆境适应性Horticulture——Quality Improvement & Stress adaptation

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For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Identification of key genes involved in flavonoid and terpenoid biosynthesis and the pathway of triterpenoid biosynthesis in Passiflora edulis XU Yi, HUANG Dong-mei, MA Fu-ning, YANG Liu, WU Bin, XING Wen-ting, SUN Pei-guang, CHEN Di, XU Bing-qiang, SONG Shun 2023, 22 (5): 1412-1423.   DOI: 10.1016/j.jia.2023.03.005 Abstract （348）      PDF in ScienceDirect       Passion fruit (Passiflora edulis Sims) is a vine of the Passiflora genus in the Passifloraceae family.  The extracted components include flavonoids and terpenoids, which have good anti-anxiety and anti-inflammatory effects in humans.  In this study, we analyzed the transcriptomes of four tissues of the ‘Zixiang’ cultivar using RNA-Seq, which provided a dataset for functional gene mining.  The de novo assembly of these reads generated 96 883 unigenes, among which 61 022 unigenes were annotated (62.99% yield).  In addition to its edible value, another important application of passion fruit is its medicinal value.  The flavonoids and terpenoids are mainly derivatives of luteolin, apigenin, cycloartane triterpenoid saponins and other active substances in leaf extracts.  A series of candidate unigenes in the transcriptome data that are potentially involved in the flavonoid and terpenoid synthesis pathways were screened using homology-based BLAST and phylogenetic analysis.  The results showed that the biosynthesis of triterpenoids in passion fruit comes from the branches of the mevalonate (MVA) and 2-C-methyl-D-erythritol 4-phosphate/1-deoxy-D-xylulose 5-phosphate (MEP/DOXP) pathways, which is different from the MVA pathway that is used in other fruit trees.  Most of the candidate genes were found to be highly expressed in the leaves and/or flowers.  Quantitative real-time PCR (qRT-PCR) verification was carried out and confirmed the reliability of the RNA-Seq data.  Further amplification and functional analysis of these putative unigenes will provide additional insight into the biosynthesis of flavonoids and terpenoids in passion fruit. Reference | Related Articles | Metrics Select Cassava MeRS40 is required for the regulation of plant salt tolerance MA Xiao-wen, MA Qiu-xiang, MA Mu-qing, CHEN Yan-hang, GU Jin-bao, LI Yang, HU Qing, LUO Qing-wen, WEN Ming-fu, ZHANG Peng, LI Cong, WANG Zhen-yu 2023, 22 (5): 1396-1411.   DOI: 10.1016/j.jia.2023.04.003 Abstract （318）      PDF in ScienceDirect       Soil salinity affects the expression of serine/arginine-rich (SR) genes and isoforms by alternative splicing, which in turn regulates the adaptation of plants to stress.  We previously identified the cassava spliceosomal component 35 like (SCL) and SR subfamilies, belonging to the SR protein family, which are extensively involved in responses to abiotic stresses.  However, the post-transcriptional regulatory mechanism of cassava arginine/serine-rich (RS) subfamily in response to salt stress remains to be explored.  In the current study, we identified 37 genes of the RS subfamily from 11 plant species and systematically investigated the transcript levels of the RS40 and RS31 genes under diverse abiotic stress conditions.  Subsequently, an analysis of the conserved protein domains revealed that plant RS subfamily genes were likely to preserve their conserved molecular functions and played critical functional roles in responses to abiotic stresses.  Importantly, we found that overexpression of MeRS40 in Arabidopsis enhanced salt tolerance by maintaining reactive oxygen species homeostasis and up-regulating the salt-responsive genes.  However, overexpression of MeRS40 gene in cassava reduced salt tolerance due to the depression of its endogenous gene expression by negative autoregulation of its own pre-mRNA.  Moreover, the MeRS40 protein interacted with MeU1-70Ks (MeU1-70Ka and MeU1-70Kb) in vivo and in vitro, respectively.  Therefore, our findings highlight the critical role of cassava SR proteins in responses to salt stress in plants.  Reference | Related Articles | Metrics Select Physiological and transcriptome analyses provide new insights into the mechanism mediating the enhanced tolerance of melatonin-treated rhododendron plants to heat stress XU Yan-xia, ZHANG Jing, WAN Zi-yun, HUANG Shan-xia, DI Hao-chen, HE Ying, JIN Song-heng 2023, 22 (8): 2397-2411.   DOI: 10.1016/j.jia.2023.07.005 Abstract （318）      PDF in ScienceDirect       Rhododendron is a well-known genus consisting of commercially valuable ornamental woody plant species.  Heat stress is a major environmental factor that affects rhododendron growth.  Melatonin was recently reported to alleviate the effects of abiotic stress on plants.  However, the role of melatonin in rhododendron plants is unknown.  In this study, the effect of melatonin on rhododendron plants exposed to heat stress and the potential underlying mechanism were investigated.  Analyses of morphological characteristics and chlorophyll a fluorescence indicated 200 µmol L–1 was the optimal melatonin concentration for protecting rhododendron plants from heat stress.  To elucidate how melatonin limits the adverse effects of high temperatures, melatonin contents, photosynthetic indices, Rubisco activity, and adenosine triphosphate (ATP) contents were analyzed at 25, 35, and 40°C, respectively.  Compared with the control, exogenous application of melatonin improved the melatonin contents, electron transport rate, photosystem II and I activities, Rubisco activity, and ATP contents under heat stress.  The transcriptome analysis revealed many of the heat-induced differentially expressed genes were associated with the photosynthetic pathway; the expression of most of these genes was down-regulated by heat stress more in the melatonin-free plants than in the melatonin-treated plants.  We identified RhPGR5A, RhATPB, RhLHCB3, and RhRbsA as key genes.  Thus, we speculate that melatonin promotes photosynthetic electron transport, improves Calvin cycle enzyme activities, and increases ATP production.  These changes lead to increased photosynthetic efficiency and CO2 assimilation under heat stress conditions via the regulated expression of specific genes, including RhRbsA.  Therefore, the application of exogenous melatonin may increase the tolerance of rhododendron to heat stress. Reference | Related Articles | Metrics Select MdWRKY40is directly promotes anthocyanin accumulation and blocks MdMYB15L, the repressor of MdCBF2, which improves cold tolerance in apple XU Peng-yue, XU Li, XU Hai-feng, HE Xiao-wen, HE Ping, CHANG Yuan-sheng, WANG Sen, ZHENG Wen-yan, WANG Chuan-zeng, CHEN Xin, LI Lin-guang, WANG Hai-bo 2023, 22 (6): 1704-1719.   DOI: 10.1016/j.jia.2023.04.033 Abstract （292）      PDF in ScienceDirect       Cold stress is an important factor that limits apple production.  In this study, we examined the tissue-cultured plantlets of apple rootstocks ‘M9T337’ and ‘60-160’, which are resistant and sensitive to cold stress, respectively.  The enriched pathways of differentially expressed genes (DEGs) and physiological changes in ‘M9T337’ and ‘60-160’ plantlets were clearly different after cold stress (1°C) treatment for 48 h, suggesting that they have differential responses to cold stress.  The differential expression of WRKY transcription factors in the two plantlets showed that MdWRKY40is and MdWRKY48 are potential regulators of cold tolerance.  When we overexpressed MdWRKY40is and MdWRKY48 in apple calli, the overexpression of MdWRKY48 had no significant effect on the callus, while MdWRKY40is overexpression promoted anthocyanin accumulation, increased callus cold tolerance, and promoted the expression of anthocyanin structural gene MdDFR and cold-signaling core gene MdCBF2.  Yeast one-hybrid screening and electrophoretic mobility shift assays showed that MdWRKY40is could only bind to the MdDFR promoter.  Yeast two-hybrid screening and bimolecular fluorescence complementation showed that MdWRKY40is interacts with the CBF2 inhibitor MdMYB15L through the leucine zipper (LZ).  When the LZ of MdWRMY40is was knocked out, MdWRKY40is overexpression in the callus did not affect MdCBF2 expression or callus cold tolerance, indicating that MdWRKY40is acts in the cold signaling pathway by interacting with MdMYB15L.  In summary, MdWRKY40is can directly bind to the MdDFR promoter in order to promote anthocyanin accumulation, and it can also interact with MdMYB15L to interfere with its inhibitory effect on MdCBF2, indirectly promoting MdCBF2 expression, and thereby improving cold tolerance.  These results provide a new perspective for the cold-resistance mechanism of apple rootstocks and a molecular basis for the screening of cold-resistant rootstocks. Reference | Related Articles | Metrics Select Physiological and transcriptome analyses of Chinese cabbage in response to drought stress Lin Chen, Chao Li, Jiahao Zhang, Zongrui Li, Qi Zeng, Qingguo Sun, Xiaowu Wang, Limin Zhao, Lugang Zhang, Baohua Li 2024, 23 (7): 2255-2269.   DOI: 10.1016/j.jia.2024.03.067 Abstract （271）      PDF in ScienceDirect       Chinese cabbage is an important leafy vegetable crop with high water demand and susceptibility to drought stress.  To explore the molecular mechanisms underlying the response to drought, we performed a transcriptome analysis of drought-tolerant and -sensitive Chinese cabbage genotypes under drought stress, and uncovered core drought-responsive genes and key signaling pathways.  A co-expression network was constructed by a weighted gene co-expression network analysis (WGCNA) and candidate hub genes involved in drought tolerance were identified.  Furthermore, abscisic acid (ABA) biosynthesis and signaling pathways and their drought responses in Chinese cabbage leaves were systemically explored.  We also found that drought treatment increased the antioxidant enzyme activities and glucosinolate contents significantly.  These results substantially enhance our understanding of the molecular mechanisms underlying drought responses in Chinese cabbage. Reference | Related Articles | Metrics Select Blue light induces leaf color change by modulating carotenoid metabolites in orange-head Chinese cabbage (Brassica rapa L. ssp. pekinensis) ZHANG Rui-xing, ZHANG Ni-nan, WANG Ya-xiu, Khan ABID, MA Shuai, BAI Xue, ZENG Qi, PAN Qi-ming, LI Bao-hua, ZHANG Lu-gang 2023, 22 (11): 3296-3311.   DOI: 10.1016/j.jia.2023.09.029 Abstract （188）      PDF in ScienceDirect       Carotenoids are involved in the formation of plant leaf color as well as photosystem photoprotection.  This study showed that blue light significantly induced up-regulation of the total carotenoid content in the inner leaves of orange-head Chinese cabbage (OHCC).  Furthermore, the transcriptomic analysis revealed that blue light treatment induced up-regulation of genes in photosynthesis (BrHY5-2, BrCOP1 and BrDET1) and the methylerythritol 4-phosphate pathways (BrGGPS, BrDXS and BrHDR) upstream of the carotenoid metabolic pathway.  Carotenoid metabolomic analysis revealed that the accumulation of several orange and red carotenoids (lycopene, zeaxanthin, β-carotene, lutein, and β-cryptoxanthin) after blue light treatment contributed to the deepening of the leaf coloration, suggesting that short-term blue light treatment could be used to boost nutritional quality.  The light signal gene BrHY5-2 participated in the blue light-induced transcriptional regulation of carotenoid biosynthesis in OHCC.  Overexpression of BrHY5-2 in Arabidopsis significantly increased the total carotenoid content and the sensitivity to blue light.  The above findings revealed new insights about blue-light-induced carotenoid synthesis and accumulation in OHCC lines.  They suggested a new engineering approach to increase the nutritional value of vegetables. Reference | Related Articles | Metrics Select Nitrogen application regulates antioxidant capacity and flavonoid metabolism, especially quercetin, in grape seedlings under salt stress Congcong Zhang, Han Wang, Guojie Nai, Lei Ma, Xu Lu, Haokai Yan, Meishuang Gong, Yuanyuan Li, Ying Lai, Zhihui Pu, Li Wei, Guiping Chen, Ping Sun, Baihong Chen, Shaoying Ma, Sheng Li 2024, 23 (12): 4074-4092.   DOI: 10.1016/j.jia.2024.07.013 Abstract （174）      PDF in ScienceDirect       Salt stress is a typical abiotic stress in plants that causes slow growth, stunting, and reduced yield and fruit quality.  Fertilization is necessary to ensure proper crop growth.  However, the effect of fertilization on salt tolerance in grapevine is unclear.  In this study, we investigated the effect of nitrogen fertilizer (0.01 and 0.1 mol L–1 NH4NO3) application on the salt (200 mmol L–1 NaCl) tolerance of grapevine based on physiological indices, and transcriptomic and metabolomic analyses.  The results revealed that 0.01 mol L–1 NH4NO3 supplementation significantly reduced the accumulation of superoxide anion (O2–·), enhanced the activities of superoxide dismutase (SOD) and peroxidase (POD), and improved the levels of ascorbic acid (AsA) and glutathione (GSH) in grape leaves compared to salt treatment alone.  Specifically, joint transcriptome and metabolome analyses showed that the differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were significantly enriched in the flavonoid biosynthesis pathway (ko00941) and the flavone and flavonol biosynthesis pathway (ko00944).  In particular, the relative content of quercetin (C00389) was markedly regulated by salt and nitrogen.  Further analysis revealed that exogenous foliar application of quercetin improved the SOD and POD activities, increased the AsA and GSH contents, and reduced the H2O2 and O2–· contents.  Meanwhile, 10 hub DEGs, which had high Pearson correlations (R2>0.9) with quercetin, were repressed by nitrogen.  In conclusion, all the results indicated that moderate nitrogen and quercetin application under salt stress enhanced the antioxidant system defense response, thus providing a new perspective for improving salt tolerance in grapes. Reference | Related Articles | Metrics Select High-throughput screening system of citrus bacterial canker-associated transcription factors and its application to the regulation of citrus canker resistance Jia Fu, Jie Fan, Chenxi Zhang, Yongyao Fu, Baohang Xian, Qiyuan Yu, Xin Huang, Wen Yang, Shanchun Chen, Yongrui He, Qiang Li 2024, 23 (1): 155-165.   DOI: 10.1016/j.jia.2023.11.011 Abstract （171）      PDF in ScienceDirect       One of the main diseases that adversely impacts the global citrus industry is citrus bacterial canker (CBC), caused by the bacteria Xanthomonas citri subsp. citri (Xcc).  Response to CBC is a complex process, with both protein-DNA as well as protein–protein interactions for the regulatory network.  To detect such interactions in CBC resistant regulation, a citrus high-throughput screening system with 203 CBC-inducible transcription factors (TFs), were developed.  Screening the upstream regulators of target by yeast-one hybrid (Y1H) methods was also performed.  A regulatory module of CBC resistance was identified based on this system.  One TF (CsDOF5.8) was explored due to its interactions with the 1-kb promoter fragment of CsPrx25, a resistant gene of CBC involved in reactive oxygen species (ROS) homeostasis regulation.  Electrophoretic mobility shift assay (EMSA), dual-LUC assays, as well as transient overexpression of CsDOF5.8, further validated the interactions and transcriptional regulation.  The CsDOF5.8–CsPrx25 promoter interaction revealed a complex pathway that governs the regulation of CBC resistance via H2O2 homeostasis.  The high-throughput Y1H/Y2H screening system could be an efficient tool for studying regulatory pathways or network of CBC resistance regulation.  In addition, it could highlight the potential of these candidate genes as targets for efforts to breed CBC-resistant citrus varieties. Reference | Related Articles | Metrics Select Overexpression of PbrGA2ox1 enhances pear drought tolerance through the regulation of GA3-inhibited reactive oxygen species detoxification and abscisic acid signaling Guoling Guo, Haiyan Zhang, Weiyu Dong, Bo Xu, Youyu Wang, Qingchen Zhao, Lun Liu, Xiaomei Tang, Li Liu, Zhenfeng Ye, Wei Heng, Liwu Zhu, Bing Jia 2024, 23 (9): 2989-3011.   DOI: 10.1016/j.jia.2024.01.012 Abstract （170）      PDF in ScienceDirect       Drought stress is a devastating natural disaster driven by the continuing intensification of global warming, which seriously threatens the productivity and quality of several horticultural crops, including pear.  Gibberellins (GAs) play crucial roles in plant growth, development, and responses to drought stress.  Previous studies have shown significant reductions of GA levels in plants under drought stress; however, our understanding of the intrinsic regulation mechanisms of GA-mediated drought stress in pear remains very limited.  Here, we show that drought stress can impair the accumulation of bioactive GAs (BGAs), and subsequently identified PbrGA2ox1 as a chloroplast-localized GA deactivation gene.  This gene was significantly induced by drought stress and abscisic acid (ABA) treatment, but was suppressed by GA3 treatment.  PbrGA2ox1-overexpressing transgenic tobacco plants (Nicotiana benthamiana) exhibited enhanced tolerance to dehydration and drought stresses, whereas knock-down of PbrGA2ox1 in pear (Pyrus betulaefolia) by virus-induced gene silencing led to elevated drought sensitivity.  Transgenic plants were hypersensitive to ABA, and had a lower BGAs content, enhanced reactive oxygen species (ROS) scavenging ability, and augmented ABA accumulation and signaling under drought stress compared to wild-type plants.  However, the opposite effects were observed with PbrGA2ox1 silencing in pear.  Moreover, exogenous GA3 treatment aggravated the ROS toxic effect and restrained ABA synthesis and signaling, resulting in the compromised drought tolerance of pear.  In summary, our results shed light on the mechanism by which BGAs are eliminated in pear leaves under drought stress, providing further insights into the mechanism regulating the effects of GA on the drought tolerance of plants. Reference | Related Articles | Metrics Select Effects of water and fertilizer deficit regulation with drip irrigation at different growth stages on fruit quality improvement of kiwifruit in seasonal arid areas of Southwest China ZHA Yu-xuan, CHEN Fei, WANG Zhi-hui, JIANG Shou-zheng, CUI Ning-bo 2023, 22 (10): 3042-3058.   DOI: 10.1016/j.jia.2023.08.015 Abstract （146）      PDF in ScienceDirect       For a long time, seasonal drought occurs frequently in Southwest China, and the management of water and fertilizer in kiwifruit orchards has no quantitative standards, which seriously affects the yield and quality of kiwifruit.  Therefore, the effects of water and fertilizer deficit regulation with drip irrigation (WFDRDI) on the quality of kiwifruit at different growth stages were explored to achieve water and fertilizer saving, and green and efficient production of kiwifruit.  We select ‘Jin Yan’ kiwifruit and set two water deficit levels (WD20% and WD40%) and three fertilizer deficit levels (FD15%, FD30% and FD45%) at bud burst to leafing stage (stage I), flowering to fruit set stage (stage II), fruit expansion stage (stage III) and fruit maturation stage (stage IV), respectively, with a full irrigation and fertilization as the control treatment (CK) in 2017 and 2018.  Results showed that the WFDRDI at stage II and III had significant effect on fruit physical quality of kiwifruit, specifically, the III-WD40%FD30% and III-WD20%FD45% treatments significantly increased fruit firmness by 13.62 and 15.59% (P<0.05), respectively; the II-WD40%FD15% and III-WD40%FD15% treatments significantly increased dry matter by 8.19 and 6.47% (P<0.05), respectively; the III-WD20%FD15% treatment significantly increased single fruit weight and fruit volume by 9.33 and 12.65% (P<0.05), respectively; the II-WD20%FD15% treatment significantly increased fruit water content by 1.99% (P<0.05).  The WFDRDI had an obvious effect on fruit chemical quality of kiwifruit.  The III-WD20%FD45%, IV-WD40%FD15% and IV-WD20%FD30% treatments significantly increased vitamin C (Vc) content by 69.96, 36.96 and 34.31% (P<0.05), respectively; the III-WD40%FD15% and IV-WD40%FD15% treatments significantly increased total soluble solid (TSS) content by 3.79 and 17.05% (P<0.05), respectively, and significantly increased soluble sugar content by 28.61 and 34.79% (P<0.05), respectively; the contents of fructose, glucose and sucrose also had a significantly increasing trend, which was increased significantly by 5.58–19.63%, 40.55–60.36% and 54.03–54.92% in the III-WD40%FD15% and IV-WD40%FD15% treatments (P<0.05), respectively; sugar–acid ratio was increased significantly in the IV-WD40%FD15% treatment by 64.65% (P<0.05).  The degree and duration of water and fertilizer deficit had a comprehensive effect on fruit quality of kiwifruit.  The WFDRDI at stage II and III contribute to improving fruit physical quality, and the threshold of water and fertilizer deficit were 20 and 15%, respectively; stage III and IV are the critical periods for improving fruit chemical quality by water and fertilizer coupling effect, and the threshold of water and fertilizer deficit were 40 and 15%, respectively.  Therefore, aiming at precise water and fertilizer saving, the I-WD20%FD30%, II-WD40%FD15%, III-WD40%FD15% and IV-WD40%FD15% treatments under WFDRDI during the whole growth period of kiwifruit were the best mode to improve quality and production of kiwifruit. Reference | Related Articles | Metrics Select Suppression of CsFAD3 in a JA-dependent manner, but not through the SA pathway, impairs drought stress tolerance in tea Na Chang, Xiaotian Pi, Ziwen Zhou, Yeyun Li, Xianchen Zhang 2024, 23 (11): 3737-3750.   DOI: 10.1016/j.jia.2024.04.002 Abstract （90）      PDF in ScienceDirect       The growth and yield of tea plants are seriously limited by drought stress.  Fatty acid desaturases (FADs) contribute to the mediation of membrane fluidity in response to different stresses, although the role of ω-3 FAD (Omega-3 fatty acid desaturase)-mediated damage induced by drought stress in tea plants is poorly understood.  In this study, drought stress significantly promoted the synthesis of C18:3 (linolenic acid) and the expression level of CsFAD3.  Yeast experiments further demonstrated that CsFAD3 can convert C18:2 to C18:3, and that the 35S:GFP-CsFAD3 fusion protein was localized in the endoplasmic reticulum of Nicotiana benthamiana cells.  CsFAD3-silenced tea leaves exhibited poor drought tolerance, with a lower Fv/Fm and a higher malondialdehyde (MDA) content than the control plants.  However, transgenic 35S:CsFAD3 Arabidopsis plants showed the opposite phenotypes.  In addition, the jasmonic acid (JA) content and the expression levels of CsLOX2, CsLOX4, CsAOS, CsAOC3 and CsOPR2 were significantly reduced in CsFAD3-silenced leaves under drought stress.  However, no substantial difference in the salicylic acid (SA) content was detected under normal or drought conditions.  An analysis of Atcoi1 (JA receptor) or Atnpr1 (SA receptor) mutant Arabidopsis plants in 35S:CsFAD3 backgrounds further revealed that knockout of Atcoi1 impaired the drought-tolerant phenotypes of CsFAD3 overexpression lines.  Therefore, this study demonstrated that CsFAD3 plays a crucial role in drought tolerance by mediating JA pathways. Reference | Related Articles | Metrics Select Dopamine improves apple replant disease resistance by regulating physiological resilience and rhizosphere microbial community structure Peihua Du, Yang Cao, Huaite Liu, Jiahao Ji, Wei Sun, Xueying Zhang, Jizhong Xu, Bowen Liang 2024, 23 (9): 3025-3044.   DOI: 10.1016/j.jia.2024.07.011 Abstract （86）      PDF in ScienceDirect       Apple replant disease (ARD) is a complex agricultural problem caused by multiple stressors that can lead to increased reactive oxygen species (ROS) levels and limited nutrient utilization in plants.  However, existing countermeasures cannot effectively address this challenge.  Here, we used Malus hupehensis as a test organism to investigate whether the pleiotropic molecule dopamine can alleviate ARD using pot experiments.  Exogenous application of 100 μmol L–1 dopamine significantly promoted the growth of apple seedlings in the replanted soil, with a relative growth rate increase of 17.44%.  Our results revealed two major pathways by which dopamine regulates ARD resistance in apple trees.  First, dopamine effectively reduces the level of ROS and activates the expression of genes related to nitrogen (N) transport and metabolism.  Among those genes, MdNLP5, MdNRT1.1, MdNLP2, MdNRT2.5, MdNLP3, MdNRT2.4, MdNADH-GAGOT, and MdFd-GAGOT were strongly regulated by dopamine.  These regulatory effects promoted the uptake and utilization of soil N by the plants.  Second, dopamine improved the physical and chemical properties, enhanced microbial community diversity, and promoted mutual cooperation between microbial communities in the soil.  Furthermore, dopamine altered the microbial structure of rhizosphere soil (upregulating Clostridiales, Gaiellales, Sordariales and Mortierellales; downregulating Micrococcales, Longimicrobiales, Hypocreales and Cystobasidiales).  Notably, dopamine significantly upregulated the abundances of Gaiella and Mortierella, both of which were positively correlated with soil urease activity, soil available N content, plant growth and N uptake.  Dopamine also significantly downregulated the abundance of the plant pathogen Gibberella (by 11.71-fold) in replant soil.  Our results provide insights into the mechanisms by which dopamine promotes ARD resistance, and can promote the sustainable development of the apple industry. Reference | Related Articles | Metrics Select Melatonin and dopamine alleviate waterlogging stress in apples by recruiting beneficial endophytes to enhance physiological resilience Yang Cao, Peihua Du, Yuwei Shang, Jiahao Ji, Leiqing Tan, Xue Zhang, Jizhong Xu, Bowen Liang 2024, 23 (7): 2270-2291.   DOI: 10.1016/j.jia.2023.12.012 Abstract （85）      PDF in ScienceDirect       Melatonin and dopamine can potentially prevent waterlogging stress in apples.  The current study investigated the mechanism by which melatonin and dopamine alleviate apple waterlogging stress.  This study demonstrated that melatonin and dopamine alleviated waterlogging by removing reactive oxygen species (ROS), and that the nitric oxide (NO) content and nitrate reductase (NR) activity were significantly correlated.  Melatonin and dopamine were also found to recruit different candidate beneficial endophytes (melatonin: Novosphingobium, Propionivibrio, and Cellvibrio; dopamine: Hydrogenophaga, Simplicispira, Methyloversatilis, Candidatus_Kaiserbacteria, and Humicola), and these endophytes were significantly and positively correlated with plant growth.  Network analyses showed that melatonin and dopamine significantly affected the endophytic bacterial and fungal communities under waterlogging stress.  The metabolomic results showed that melatonin and dopamine led to waterlogging resistance by upregulating the abundance of beneficial substances such as amino acids, flavonoids, coumarins, and organic acids.  In addition, melatonin and dopamine regulated the physicochemical properties of the soil, which altered the endophyte community and affected plant growth.  The co-occurrence network demonstrated close and complex relationships among endophytes, metabolites, soil, and the plants.  Our results demonstrate that melatonin and dopamine alleviate waterlogging stress in apples by recruiting beneficial endophytes to enhance physiological resilience.  This study provides new insights into how melatonin and dopamine alleviate stress and a theoretical basis for synergistic beneficial microbial resistance to waterlogging stress. 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