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Proboscipedia and Sex combs reduced are essential for embryonic labial palpus specification in Bombyx mori
ZHANG Ru, ZHANG Zhong-jie, YU Ye, HUANG Yong-ping, QIAN Ai-rong, TAN An-jiang
2020, 19 (6): 1482-1491.   DOI: 10.1016/S2095-3119(19)62785-1
Abstract109)      PDF in ScienceDirect      
Hox genes of proboscipedia (pb) and Sex combs reduced (Scr) and their cofactors are required for determination of insect mouthpart identity and play important roles in insect organ morphogenesis.  However, in the lepidopteran insects, including many agricultural and forest pests, the roles of these genes in determination of mouthpart morphogenesis are unclear.  Here we report that both BmPB (Gene ID: 101740380) and BmSCR (Gene ID: 692761) are essential for labial palpus specification in a lepidopteran model insect, the silkworm, Bombyx mori.  During embryonic morphogenesis, CRISPR/Cas9-mediated mutagenesis of BmPB induced transformation of labial palpus into thoracic leg and BmSCR mutation transformed labial palpus into maxilla.  Mutagenesis of Hox-related cofactor extradenticle (BmEXD; Gene ID: 692478) and its nuclear localization factor homothorax (BmHTH; Gene ID: 576938425) also induced distortion of embryonic labial palpus.  Furthermore, mutagenesis of each of the four genes induced severe degeneration of spigot morphogenesis, an important part of spinneret structure.  Quantitative real-time PCR analysis further revealed that the transcriptional interaction among these genes.  Our data thus provide novel evidence for Hox gene regulation of insect embryonic patterning. 
 
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Optimizing nitrogen application and planting density improves yield and resource use efficiency via regulating canopy light and nitrogen distribution in rice
Zichen Liu, Liyan Shang, Shuaijun Dai, Jiayu Ye, Tian Sheng, Jun Deng, Ke Liu, Shah Fahad, Xiaohai Tian, Yunbo Zhang, Liying Huang
DOI: 10.1016/j.jia.2024.04.006 Online: 30 April 2024
Abstract21)      PDF in ScienceDirect      
Coordinating light and nitrogen (N) distribution within a canopy is crucial to improve rice yield and resource use efficiency.  However, little attention has been paid to light and N distribution in response to planting density and N rate, and its relationships with grain yield, radiation use efficiency (RUE), and N use efficiency for grain production (NUEg) in rice.  Here, a two-year field experiment was conducted with two hybrid varieties under three N levels, 0 (N1), 90 (N2) and 180 kg ha-1 (N3), and two planting densities, 22.2 (D1) and 33.3 hills m-2 (D2).  On average, a 3.4% higher yield and 4.4% higher NUEg were observed under N2D2 compared with N3D1.  The extinction coefficient for N (KN) and light (KL) and their ratio (KN/KL) at the heading stage were significantly affected by the N rate, planting density, and their interaction.  KN decreased with the increase of N input or planting density.  Compared with N1, KN decreased by 43.5 and 58.8% under N2 and N3, respectively, and KN under D2 decreased by 16.0% compared with D1.  Higher KL and KN/KL values were observed under a low N rate, while the opposite trend was shown under a high N rate.  Moreover, increasing planting density resulted in a decrease in KL and KN/KL values.  Compared with N3D1, N2D2 had higher KL and KN, and thus comparable KN/KL.  Correlation analysis further revealed that KL was negatively correlated with RUE, while KN and KN/KL were positively correlated with NUEg.  Therefore, increasing planting density under reduced N input could ensure rice yield while improving resource use efficiency via regulating canopy light and N distribution. 
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Exploring strategies for agricultural sustainability in super hybrid rice using the food-carbon-nitrogen-water-energy-profit nexus framework
Jun Deng, Ke Liu, Xiangqian Feng, Jiayu Ye, Matthew Tom Harrison, Peter de Voil, Tajamul Hussain, Liying Huang, Xiaohai Tian, Meixue Zhou, Yunbo Zhang
DOI: 10.1016/j.jia.2024.07.025 Online: 19 July 2024
Abstract36)      PDF in ScienceDirect      

The breakthrough in super hybrid rice yield has significantly contributed to China's and global food security.  However, the inherent conflict between high productivity and environmentally sustainable agriculture poses challenges. Issues like water scarcity, energy crises, escalating greenhouse gas emissions, and diminishing farm profitability all threaten agricultural sustainability.  In response to these challenges, we applied a holistic food-carbon-nitrogen-water-energy-profit (FCNWEP) nexus framework to comprehensively evaluate sustainability of distinct crop management strategies across three sub-sites in central China.  Field experiments were conducted in Hubei and Hunan Provinces from 2017 to 2021, with a widely adopted elite super hybrid rice (Y-liangyou900).  Four crop management treatments were implemented: a control (CK, 0 kg N ha-1), conventional crop management (CCM, 210-250 kg N ha-1, 7:3 basal: mid-tiller fertilizer ratio), and two integrated crop management treatments (ICM1, 180-210 kg N ha-1, 5:2:3 basal: mid-tiller: panicle initiation fertilizer ratio; ICM2, 240-270 kg N ha-1, 5:2:2:1 basal: mid-tiller: panicle initiation: flowering fertilizer ratio).  Grain yield, carbon footprint, nitrogen footprint, energy footprint, nitrogen use efficiency and economic benefits were among the assessed variables.  Our results showed that significant yield variations were observed, with ICM2 consistently outperforming CCM and ICM1 across the three sites.  In Jingzhou, Suizhou, and Changsha, ICM2's grain yield was 30.2, 24.7, and 13.3% higher than CCM, respectively.  Additionally, net profits for ICM2 exceeded those of CCM and ICM1 by 31.8 and 115.2% in Jingzhou, 32.2 and 109.9% in Suizhou, and 15.4 and 34.0% in Changsha.  Integrated crop managements, specifically ICM2, demonstrated improved nitrogen and energy use efficiency, resulting in reduced carbon, nitrogen, water, and energy footprints.  Overall, composite sustainability scores, calculated using the FCNWEP framework, indicated that both ICM2 and ICM1 generally exhibited higher sustainability levels compared to CCM.  This study offers valuable insights into practical management methodologies and provides recommendations for enhancing agricultural sustainability.

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Automatic diagnosis of agromyzid leafminer damage levels using leaf images captured by AR glasses
Zhongru Ye, Yongjian Liu, Fuyu Ye, Hang Li, Ju Luo, Jianyang Guo, Zelin Feng, Chen Hong, Lingyi Li, Shuhua Liu, Baojun Yang, Wanxue Liu, Qing Yao
DOI: 10.1016/j.jia.2025.02.008 Online: 10 February 2025
Abstract11)      PDF in ScienceDirect      

Agromyzid leafminers cause significant economic losses in both vegetable and horticultural crops, and precise assessments of pesticide needs must be based on the extent of leaf damage. Traditionally, surveyors estimate the damage by visually comparing the proportion of damaged to intact leaf area, a method that lacks objectivity, precision, and reliable data traceability. To address these issues, an advanced survey system that combines augmented reality (AR) glasses with a camera and an artificial intelligence (AI) algorithm was developed in this study to objectively and accurately assess leafminer damage in the field. By wearing AR glasses equipped with a voice-controlled camera, surveyors can easily flatten damaged leaves by hand and capture images for analysis. This method can provide a precise and reliable diagnosis of leafminer damage levels, which in turn supports the implementation of scientifically grounded and targeted pest management strategies. To calculate the leafminer damage level, the DeepLab-Leafminer model was proposed to precisely segment the leafminer-damaged regions and the intact leaf region. The integration of an edge-aware module and a Canny loss function into the DeepLabv3+ model enhanced the DeepLab-Leafminer model's capability to accurately segment the edges of leafminer-damaged regions, which often exhibit irregular shapes. Compared with state-of-the-art segmentation models, the DeepLab-Leafminer model achieved superior segmentation performance with an Intersection over Union (IoU) of 81.23% and an F1 score of 87.92% on leafminer-damaged leaves. The test results revealed a 92.38% diagnosis accuracy of leafminer damage levels based on the DeepLab-Leafminer model. A mobile application and a web platform were developed to assist surveyors in displaying the diagnostic results of leafminer damage levels. This system provides surveyors with an advanced, user-friendly, and accurate tool for assessing agromyzid leafminer damage in agricultural fields using wearable AR glasses and an AI model. This method can also be utilized to automatically diagnose pest and disease damage levels in other crops based on leaf images.

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