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SlGH9-15 regulates tomato fruit cracking with hormonal and abiotic stress responsiveness cis-elements

LIN Hao-wei, WU Zhen, ZHOU Rong, CHEN Bin, ZHONG Zhao-jiang, JIANG Fang-ling

2023, 22 (2): 447-463. DOI: 10.1016/j.jia.2022.09.013

Abstract （313） PDF in ScienceDirect

Fruit cracking occurs easily during the late period of fruit development when plants encounter an unsuitable environment, dramatically affecting fruit production and marketing. This study conducted the bulked segregant RNA-Seq (BSR) to identify the key regulatory gene of fruit cracking in tomatoes. BSR-Seq analysis illustrated that two regions associated with irregularly cracking were located on chromosomes 9 and 11, containing 127 candidate genes. Further, through differentially expression analysis and qRT-PCR in cracking-susceptible and cracking-resistant genotypes, the candidate gene SlGH9-15 (Solyc09g010210) with significantly differential expression levels was screened. Bioinformatics analysis of the GH9 gene family revealed that 20 SlGH9 genes were divided into three groups. The phylogenetic analysis showed that SlGH9-15 was closely related to cell wall construction-associated genes AtGH9B1, AtGH9B6, OsGH9B1, and OsGH9B3. The cis-acting elements analysis revealed that SlGH9-15 was activated by various hormones (ethylene and ABA) and abiotic stresses. The expression pattern indicated that 13 SlGH9 genes, especially SlGH9-15, were highly expressed in the cracking-susceptible genotype. Its expression level gradually increased during fruit development and achieved maximum value at the red ripe stage. Additionally, the cracking-susceptible tomato showed higher cellulase activity and lower cellulose content than the cracking-resistant tomato, particularly at the red ripe stage. This study identified SlGH9-15 as a key gene associated with fruit cracking in tomatoes for the first time and gives new insights for understanding the molecular mechanism and complex regulatory network of fruit cracking

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An optimized industry processing technology of peanut tofu and the novel prediction model for suitable peanut varieties

CHEN Bing-yu, LI Qi-zhai, HU Hui, MENG Shi, Faisal SHAH, WANG Qiang, LIU Hong-zhi

2020, 19 (9): 2340-2351. DOI: 10.1016/S2095-3119(20)63249-X

Abstract （142） PDF in ScienceDirect

Peanut protein is easily digested and absorbed by the human body, and peanut tofu does not contain flatulence factors and beany flour. However, at present, there is no industrial preparation process of peanut tofu, whereas the quality of tofu prepared by different peanut varieties is quite different. This study established an industrial feasible production process of peanut tofu and optimized the key process that regulates its quality. Compared with the existing method, the production time is reduced by 53.80%, therefore the daily production output is increased by 183.33%. The chemical properties of 26 peanut varieties and the quality characteristics of tofu prepared from these 26 varieties were determined. The peanut varieties were classified based on the quality characteristics of tofu using the hierarchical cluster analysis (HCA) method, out of which 7 varieties were screened out which were suitable for preparing peanut tofu. An evaluation standard was founded based on peanut tofu qualities. Six chemical trait indexes were correlated with peanut tofu qualities (P<0.05). A logistic regressive model was developed to predict suitable peanut varieties and this prediction model was verified. This study may help broaden the peanut protein utilization, and provide guidance for breeding experts to select certain varieties for product specific cultivation of peanut.

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Genomic characteristics of Dickeya fangzhongdai isolates from pear and the function of type IV pili in the chromosome

CHEN Bin, TIAN Yan-li, ZHAO Yu-qiang, WANG Yuan-jie, CHUAN Jia-cheng, LI Xiang, HU Bai-shi

2020, 19 (4): 906-920. DOI: 10.1016/S2095-3119(19)62883-2

Abstract （151） PDF in ScienceDirect

Dickeya fangzhongdai, the causal agent of bleeding canker of pear, is a new member of the Dickeya genus and the only one that infects woody plants. Recent studies have reclassified several Dickeya isolates as D. fangzhongdai, which were isolated from various environments, including water, Phalaenopsis sp. and Aglaonema sp. To provide genomic characterization of D. fangzhongdai isolates from pear, the genomes of D. fangzhongdai strain JS5 (=China General Microbiological Culture Collection Center, CGMCC 1.15464T=DSM 101947T), along with two other isolates, LN1 and QZH3, were sequenced and compared to those of other Dickeya spp. Homology greater than 99% was observed among three D. fangzhongdai strains. Plasmid, type IV secretion system (T4SS) and type IV pili (TFPs) were found in genomes of D. fangzhongdai isolates. Comparative analysis of the type III secretion systems (T3SS), type III secretion effectors (T3SE), plant cell wall degradation enzymes (PCWDE) and membrane transport proteins of Dickeya spp. showed some differences which might reflect the variations of virulence, phylogenetic and phenotypic characteristics of Dickeya spp. In addition, deletion mutant of TFP in D. fangzhongdai JS5 showed no twitching motility and reduced virulence and biofilm formation. The fingdings of the distinctive plasmid, T4SS and TFPs, as well as the differences of T3SE, PCWDE and membrane transport proteins make D. fangzhongdai isolates unique. These results also suggested that acquisition of virulence genes by horizontal gene transfer might play some role in the genetic variation of D. fangzhongdai.

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Bleeding canker of pears caused by Dickeya fangzhongdai: Symptoms, etiology and biology

CHEN Bin, TIAN Yan-li, ZHAO Yu-qiang, WANG Jia-nan, XU Zhi-gang, LI Xiang, HU Bai-shi

2020, 19 (4): 889-897. DOI: 10.1016/S2095-3119(19)62882-0

Abstract （175） PDF in ScienceDirect

Bleeding canker, a devastating disease of pear trees (Pyrus pyrifolia L.), was first reported in the 1970s in Jiangsu, China and more recently in other provinces in China. Trees infected with bleeding canker pathogen, Dickeya fangzhongdai, develop cankers on the trunks and branches, and a rust-colored mixture of bacterial ooze and tree sap could be seen all over the trunks and branches. In this study, we provided detail descriptions of the symptoms and epidemiology of bleeding canker disease. Based on pathogenic and phenotypic characterizations, we identified the causal agent of bleeding canker of pear as D. fangzhongdai. Dickeya fangzhongdai strains isolated from pear were also pathogenic on Solanum tuberosum, Brassica pekinensis, Lycopersicon esculentum, and Phalaenopsis aphrodite based on artificial inoculation, and the pathogen were more virulent on potato than that of D. solani strain. This study provides new information about this disease and bleeding canker disease of pear.

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Potato/Maize intercropping reduces infestation of potato tuber moth, Phthorimaea operculella (Zeller) by the enhancement of natural enemies

ZHENG Ya-qiang, ZHANG Li-min, CHEN Bin, YAN Nai-sheng, GUI Fu-rong, ZAN Qing-an, DU Guang-zu, HE Shu-qi, LI Zheng-yue, GAO Yu-lin, XIAO Guan-li

2020, 19 (2): 394-405. DOI: 10.1016/S2095-3119(19)62699-7

Abstract （150） PDF in ScienceDirect

The potato tuber moth (PTM), Phthorimaea operculella (Zeller), is one of the most economically significant insect pests for potato in both field and storage worldwide. To evaluate the infestation, reduction of potato yield and the control efficacy for PTM, field tests were conducted in two seasons by intercropping of potato as the host plant with maize as a non-host plant of PTM. Three intercropping patterns were tested, which were 2 rows of potatoes with either 2, 3, or 4 rows of maize (abbreviated 2P:2M, 2P:3M, and 2P:4M), and the monocropped potato as the control, 2 rows of potatoes, without maize, (abbreviated 2P:0M). Results showed that the population and infestation of PTM in the 2P:3M intercropping pattern was significantly lower than those in 2P:2M, 2P:4M and the monocropping pattern of 2P:0M, due to the enhancement of natural enemies. Cumulative mines and tunneling in potato leaves in 2P:3M intercropping were significantly lower than those in 2P:2M and 2P:4M patterns. The population of parasitoids and the parasitism rate of PTM in intercropping pattern of 2P:3M were significantly higher than that in intercropping pattern of 2P:2M, 2P:4M and monocropping pattern of 2P:0M. We conclude that the potato intercropped with maize reduced the adult and larva populations, and reduced the damage from PTM by enhancing the number of parasitoids and the level of parasitism. The greatest population density of parasitoids and parasitism rate were in the intercropping pattern of 2 rows of potatoes with 3 rows of maize. These data indicate that the host/non-host intercropping patterns can be used as a biological control tactic against PTM by enhancing the density of natural enemies in the agro-ecosystems.

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Effects of planting patterns on yield, quality, and defoliation in machine-harvested cotton

WANG Fang-yong, HAN Huan-yong, LIN Hai, CHEN Bing, KONG Xian-hui, NING Xin-zhu, WANG Xu-wen, YU Yu, LIU Jing-de

2019, 18 (9): 2019-2028. DOI: 10.1016/S2095-3119(19)62604-3

Abstract （122） PDF in ScienceDirect

The aim of this study was to elucidate the effects of different machine-harvested cotton-planting patterns on defoliation, yield, and fiber quality in cotton and to provide support for improving the quality of machine-harvested cotton. In the 2015 and 2016 growing seasons, the Xinluzao 45 (XLZ45) and Xinluzao 62 (XLZ62) cultivars, which are primarily cultivated in northern Xinjiang, were used as study materials. Conventional wide-narrow row (WNR), wide and ultra-narrow row (UNR), wide-row spacing with high density (HWR), and wide-row spacing with low density (LWR) planting patterns were used to assess the effects of planting patterns on defoliation, yield, and fiber quality. Compared with WNR, the seed cotton yields were significantly decreased by 2.06–5.48% for UNR and by 2.50–6.99% for LWR, respectively. The main cause of reduced yield was a reduction in bolls per unit area. The variation in HWR yield was –1.07–1.07% with reduced bolls per unit area and increased boll weight, thus demonstrating stable production. In terms of fiber quality indicators, the planting patterns only showed significant effects on the micronaire value, with wide-row spacing patterns showing an increase in the micronaire values. The defoliation and boll-opening results showed that the number of leaves and dried leaves in HWR was the lowest among the four planting patterns. Prior to the application of defoliating agent and before machine-harvesting, the numbers of leaves per individual plant in HWR were decreased by 14.45 and 25.00% on average, respectively, compared with WNR, while the number of leaves per unit area was decreased by 27.44 and 36.21% on average, respectively. The rates of boll-opening and defoliation in HWR were the highest. Specifically, the boll-opening rate before defoliation and machine-harvesting in HWR was 44.54 and 5.94% higher on average than in WNR, while the defoliation rate prior to machine-harvesting was 3.45% higher on average than in WNR. The numbers of ineffective defoliated leaves and leaf trash in HWR were the lowest, decreased by 33.40 and 32.43%, respectively, compared with WNR. In conclusion, the HWR planting pattern is associated with a high and stable yield, does not affect fiber quality, promotes early maturation, and can effectively decrease the amount of leaf trash in machine-picked seed cotton, and thus its use is able to improve the quality of machine-harvested cotton.

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miR-10b promotes porcine immature Sertoli cell proliferation by targeting the DAZAP1 gene

WENG Bo, RAN Mao-liang, Cao Rong, PENG Fu-zhi, LUO Hui, GAO Hu, TANG Xiang-wei, Yang An-qi, CHEN Bin

2019, 18 (8): 1924-1935. DOI: 10.1016/S2095-3119(19)62564-5

Abstract （160） PDF in ScienceDirect

MicroRNAs (miRNAs) have been widely identified in porcine testicular tissues and implicated as crucial regulators of proliferation, apoptosis, and differentiation in porcine spermatogenesis related cells. However, the function roles of most of the miRNAs that have been identified in Sertoli cells are poorly understood. In the present study, six experiments were conducted to study the regulatory role of miR-10b in porcine immature Sertoli cells. In experiment 1, the results showed that the relative mRNA expression level of miR-10b in porcine testicular tissues decreased quadratically (P<0.001) with increasing age, while the relative mRNA expression level of DAZAP1 gene increased (P<0.001). In addition, the mRNA expression of miR-10b was negatively (P<0.01) correlated with DAZAP1 mRNA expression (r=–0.550). In experiment 2, the results from the bioinformatic analysis and a luciferase reporter assay demonstrated that miR-10b directly targeted the DAZAP1 gene in porcine immature Sertoli cells. DAZAP1 mRNA and protein expressions were both regulated (P<0.05) by miR-10b. In experiments 3 to 5, the over-expression of miR-10b or the siRNA-mediated knockdown of the DAZAP1 gene promoted (P<0.05) porcine immature Sertoli cell proliferation, as determined by the Cell Counting Kit-8 (CCK-8) assay and the 5-Ethynyl-2´-deoxyuridine (EdU) assay. However, an annexin V-FITC/PI staining assay and the expression of cell survival-related genes indicated that over-expression of miR-10b or knockdown of DAZAP1 had no effect (P>0.05) on porcine immature Sertoli cell apoptosis. In experiment 6, the co-transfection treatment results showed that miR-10b promoted (P<0.05) porcine immature Sertoli cell proliferation by targeting DAZAP1 gene. Overall, these experiments demonstrated that miR-10b promotes porcine immature Sertoli cell proliferation by targeting the DAZAP1 gene.

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miR-34c inhibits proliferation and enhances apoptosis in immature porcine Sertoli cells by targeting the SMAD7 gene

RAN Mao-liang, WENG Bo, CAO Rong, PENG Fu-zhi, LUO Hui, GAO Hu, CHEN Bin

2019, 18 (2): 449-459. DOI: 10.1016/S2095-3119(19)62612-2

Abstract （245） PDF （4350KB）（284）

MicroRNAs (miRNAs) are implicated in swine spermatogenesis via their regulations of cell proliferation, apoptosis, and differentiation. Recent studies indicated that miR-34c is indispensable in the late steps of spermatogenesis. However, whether miR-34c plays similar important roles in immature porcine Sertoli cells remain unknown. In the present study, we conducted two experiments using a completely randomised design to study the function roles of miR-34c. The results from experiment I demonstrated that the relative expression level of miR-34c in swine testicular tissues increased (P=0.0017) quadratically with increasing age, while the relative expression level of SMAD family member 7 (SMAD7 ) decreased (P=0.0009) with curve. Furthermore, miR-34c expression levels showed a significant negative correlation (P=0.013) with SMAD7 gene expression levels. The results from experiment II indicated that miR-34c directly targets the SMAD7 gene using a luciferase reporter assay, and suppresses (P<0.05) SMAD7 mRNA and protein expressions in immature porcine Sertoli cells. Overexpression of miR-34c inhibited (P<0.05) proliferation and enhanced (P<0.05) apoptosis in the immature porcine Sertoli cells, which was supported by the results from the Cell Counting Kit-8 (CCK-8) assay, the 5-Ethynyl-2´-deoxyuridine (EdU) assay, and the Annexin V-FITC/PI staining assay. Furthermore, knockdown of SMAD7 via small interfering RNA (siRNA) gave a similar result. It is concluded that miR-34c inhibits proliferation and enhances apoptosis in immature porcine Sertoli cells by targeting the SMAD7 gene.

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The effects of soil moisture and salinity as functions of groundwater depth on wheat growth and yield in coastal saline soils

ZHANG He, LI Yan1, MENG Ya-li, CAO Nan, LI Duan-sheng, ZHOU Zhi-guo, CHEN Bing-lin, DOU Fu-gen

2019, 18 (11): 2472-2482. DOI: 10.1016/S2095-3119(19)62713-9

Abstract （121） PDF in ScienceDirect

In the coastal saline soils, moisture and salinity are the functions of groundwater depth affecting crop growth and yield. Accordingly, the objectives of this study were to: 1) investigate the combined effects of moisture and salinity stresses on wheat growth as affected by groundwater depth, and 2) find the optimal groundwater depth for wheat growth in coastal saline soils. The groundwater depths (0.7, 1.1, 1.5, 1.9, 2.3, and 2.7 m during 2013–2014 (Y1) and 0.6, 1.0, 1.4, 1.8, 2.2, and 2.6 m during 2014–2015 (Y2)) of the field experiment were maintained by soil columns. There was a positive correlation between soil moisture and salinity. Water logging with high salinity (groundwater depth at 0.7 m in Y1 and 0.6 m in Y2) showed a greater decline towards wheat growth than that of slight drought with medium (2.3 m in Y1) or low salinity (2.7 m in Y1, 2.2 and 2.6 m in Y2). The booting stage was the most sensitive stage of wheat crop under moisture and salinity stresses. Data showed the most optimal rate of photosynthesis, grain yield, and flour quality were obtained under the groundwater depth (ditch depth) of 1.9 m (standard soil moisture with medium salinity) and 2.3 m (slight drought with medium salinity) in Y1 and 1.8 m (standard soil moisture with medium salinity) and 2.2 m (slight drought with low salinity) in Y2. The corresponding optimal soil relative moisture content and conductivity with the 1:5 distilled water/soil dilution, in the depth of 0–20 cm and 20–40 cm in coastal saline soils, were equal to 58.67–63.07% and 65.51–72.66% in Y1, 63.09–66.70% and 69.75–74.72% in Y2; 0.86–1.01 dS m^–1 and 0.63–0.77 dS m^–1 in Y1, 0.57–0.93 dS m^–1 and 0.40–0.63 dS m^–1 in Y2, respectively.

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Genome-wide association study for starch content and constitution in sorghum (Sorghum bicolor (L.) Moench)

CHEN Bing-ru, WANG Chun-yu, WANG Ping, ZHU Zhen-xing, XU Ning, SHI Gui-shan, YU Miao, WANG Nai, LI Ji-hong, HOU Jia-ming, LI Shu-jie, ZHOU Yu-fei, GAO Shi-jie, LU Xiao-chun, HUANG Rui

2019, 18 (11): 2446-2456. DOI: 10.1016/S2095-3119(19)62631-6

Abstract （124） PDF in ScienceDirect

Starch is the most important component in endosperm of sorghum grain. Usually, two types of starch are present: amylose (AM) and amylopectin (AP). The levels of AM and AP contents play a significant role in the appearance, structure, and quality of sorghum grains and in marketing applications. In the present study, a panel of 634 sorghum (Sorghum bicolor (L.) Moench) accessions were evaluated for starch, AM, and AP contents of grain, which included a mini core collection of 242 accessions from the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) in India, and 252 landraces and 140 cultivars from China. The average starch content was 67.64% and the average AM and AP contents were 20.19 and 79.81%, respectively. We developed a total of 260 000 high-confidence single nucleotide polymorphism (SNP) markers in the panel of 634 accessions of S. bicolor using specific locus amplified fragment sequencing (SLAF-seq). We performed genome-wide association studies (GWAS) of starch, AM, and AM/AP of grain and SNP markers based on a mixed linear model (MLM). In total, 70 significant association signals were detected for starch, AM, and AM/AP ratio of grain with P<4.452×10^–7, of which 10 SNPs were identified with significant starch, 51 SNPs were associated with AM, and nine SNPs were associated with the AM/AP ratio. The Gene Ontology (GO) analysis identified 12 candidate genes at five QTLs associated with starch metabolism within the 200-kb intervals, located on chromosomes 1, 5, 6, and 9. Of these genes, Sobic.006G036500.1 encodes peptidyl-prolyl cis-trans-isomerase CYP38 responsible for hexose monophosphate shunt (HMS) and Sobic.009G071800 encodes 6-phospho-fructokinase (PFK), which is involved in the embden-meyerhof pathway (EMP). Kompetitive allele specific PCR (KASP) markers were developed to validate the GWAS results. The C allele is correlated with a high starch content, while the T allele is linked with a low level of starch content, and provides reliable haplotypes for MAS in sorghum quality improvement.

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Relationships between temperature-light meteorological factors and seedcotton biomass per boll at different boll positions

WU You, ZHAO Wen-qing, MENG Ya-li, WANG You-hua, CHEN Bing-lin, ZHOU Zhi-guo

2018, 17 (06): 1315-1326. DOI: 10.1016/S2095-3119(17)61820-3

Abstract （452） PDF in ScienceDirect

Cotton growth and development are determined and influenced by cultivars, meteorological conditions, and management practices. The objective of this study was to quantify the optimum of temperature-light meteorological factors for seedcotton biomass per boll with respect to boll positions. Field experiments were conducted using two cultivars of Kemian 1 and Sumian 15 with three planting dates of 25 April (mean daily temperature (MDT) was 28.0 and 25.4°C in 2010 and 2011, respectively), 25 May (MDT was 22.5 and 21.2°C in 2010 and 2011, respectively), and 10 Jun (MDT was 18.7 and 17.9°C in 2010 and 2011, respectively), and under three shading levels (crop relative light rates (CRLR) were 100, 80, and 60%) during 2010 and 2011 cotton boll development period (from anthesis to boll open stages). The main meteorological factors (temperature and light) affected seedcotton biomass per boll differently among different boll positions and cultivars. Mean daily radiation (MDR) affected seedcotton biomass per boll at all boll positions, except fruiting branch 2 (FB2) fruting node 1 (FN1). However, its influence was less than temperature factors, especially growing degree-days (GDD). Optimum mean daily maximum temperature (MDTmax) for seedcotton biomass per boll at FB11FN3 was 29.9–32.4°C, and the optimum MDR at aforementioned position was 15.8–17.5 MJ m^–2. Definitely, these results can contribute to future cultural practices such as rational cultivars choice and distribution, simplifying field managements and mechanization to acquire more efficient and economical cotton management.

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Effects of planting dates and shading on carbohydrate content, yield, and fiber quality in cotton with respect to fruiting positions

ZHAO Wen-qing, WU You, Zahoor Rizwan, WANG You-hua, MA Yi-na, CHEN Bing-lin, MENG Ya-li, ZHOU Zhi-guo

2018, 17 (05): 1106-1119. DOI: 10.1016/S2095-3119(17)61797-0

Abstract （473） PDF in ScienceDirect

Two cotton (Gossypium hirsutum L.) cultivars, Kemian 1 (cool temperature-tolerant) and Sumian 15 (cool temperature-sensitive) were used to study the effects of cool temperature on carbohydrates, yield, and fiber quality in cotton bolls located at different fruiting positions (FP). Cool temperatures were created using late planting and low light. The experiment was conducted in 2010 and 2011 using two planting dates (OPD, the optimized planting date, 25 April; LPD, the late planting date, 10 June) and two shading levels of crop relative light rate (CRLR, 100 and 60%). Compared with fruiting position 1 (FP1), cotton yield and yield components (fiber quality, leaf sucrose and starch content, and fiber cellulose) were all decreased on FP3 under all treatments. Compared with OPD-CRLR 100%, other treatments (OPD-CRLR 60%, LPD-CRLR 100%, and LPD-CRLR 60%) had significantly decreased lint yield at both FPs of both cultivars, but especially at FP3 and in Sumian 15; this decrease was mainly caused by a large decline in boll number. All fiber quality indices decreased under late planting and shading except fiber length at FP1 with OPD-CRLR 60%, and a greater reduction was observed at FP3 and in Sumian 15. Sucrose content of the subtending leaf and fiber increased under LPD compared to OPD, whereas it decreased under CRLR 60% compared to CRLR 100%, which led to decreased fiber cellulose content. Therefore, shading primarily decreased the “source” sucrose content in the subtending leaf whereas late planting diminished translocation of sucrose towards cotton fiber. Notably, as planting date was delayed and light was decreased, more carbohydrates were distributed to leaf and bolls at FP1 than those at FP3, resulting in higher yield and better fiber quality at FP1, and a higher proportion of bolls and carbohydrates allocated at FP3 of Kemian 1 compared to that of Sumian 15. In conclusion, cotton yield and fiber quality were reduced less at FP1 compared to those at FP3 under low temperature and low light conditions. Thus, reduced cotton yield and fiber quality loss can be minimized by selecting low temperature tolerant cultivars under both low temperature and light conditions.

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Susceptible time window and endurable duration of cotton fiber development to high temperature stress

XU Bo, ZHOU Zhi-guo, GUO Lin-tao, XU Wen-zheng, ZHAO Wen-qin, CHEN Bing-lin, MENG Ya-li, WANG You-hua

2017, 16 (09): 1936-1945. DOI: 10.1016/S2095-3119(16)61566-6

Abstract （796） PDF in ScienceDirect

The development of the cotton fiber is very sensitive to temperature variation, and high temperature stress often causes reduced fiber yield and fiber quality. Short-term high temperature stress often occurs during cotton production, but little is known about the specific timing and duration of stress that affects fiber development. To make this clear, pot experiments were carried in 2014 and 2015 in a climate chamber using cotton cultivars HY370WR (less sensitive variety) and Sumian 15 (heat sensitive variety), which present different temperature sensitivities. Changes of the most important fiber quality indices (i.e., fiber length, fiber strength and marcironaire) and three very important fiber development components (i.e., cellulose, sucrose and callose) were analyzed to define the time window and critical duration to the high temperature stress at 34°C (max38°C/min30°C). When developing bolls were subjected to 5 days of high temperature stress at different days post-anthesis (DPA), the changes (Δ%) of fiber length, strength and micronire, as a function of imposed time followed square polynomial eq. as y=a+bx+cx2, and the time around 15 DPA was the most sensitive period for fiber quality development in response to heat stress. When 15 DPA bolls were heat-stressed for different durations (2, 3, 4, 5, 6, 7 days), the changes (Δ%) of fiber length, strength and micronire, as a function of stress duration followed logistic equations

. Referred to that 5, 10 and 15% are usually used as criteria to decide whether techniques are effective or changes are significant in crop culture practice and reguard to the fiber quality indices change range, we suggested that 5% changes of the major fiber quality indices (fiber length, fiber strength and micronaire) and 10% changes of fiber development components (cellulose, sucrose and callose) could be taken as criteria to judge whether fiber development and fiber quality have been significantly affected by high temperature stress. The key time window for cotton fiber development in response to the high temperature stress was 13–19 DPA, and the critical duration was about 5 days.

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The effects of sowing date on cottonseed properties at different fruiting-branch positions

HU Wei, CHEN Mei-li, ZHAO Wen-qing, CHEN Bing-lin, WANG You-hua, WANG Shan-shan, MENG Ya-li, ZHOU Zhi-guo

2017, 16 (06): 1322-1330. DOI: 10.1016/S2095-3119(16)61537-X

Abstract （1236） PDF in ScienceDirect

A two-year field experiment was conducted to illustrate the effects of sowing date on cottonseed properties at different fruiting-branch positions (FBPs). Two cotton cultivars (Kemian 1 and Sumian 15) were sowed on 25 April, 25 May, and 10 June in 2010 and 2011, respectively. The boll maturation period increased with the delaying of sowing date. Normal sowing treatment (25 April) had higher seed weight, embryo weight, embryo oil content and protein content than late sowing treatments (25 May and 10 June). The flowering date, seed weight, embryo weight, embryo oil and protein contents, and the dynamic changes of embryo oil and protein contents were altered by different FBPs. A significant interaction of sowing date×FBP was observed on embryo weight, embryo oil content, embryo protein content and the dynamic changes of embryo oil and protein contents, but was not observed on seed weight. Seed weight, embryo weight, embryo oil and protein content had significant positive correlations with the mean daily temperature (MDT), mean daily maximum temperature (MDT_max), mean daily minimum temperature (MDT_min), and mean daily solar radiation (MDSR), indicating that temperature and light resources were the main reasons for different sowing dates affecting the cottonseed properties at different FBPs. Moreover, the difference in MDT was the main difference in climatic factors among different sowing dates.

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Modeling Fiber Fineness, Maturity, and Micronaire in Cotton (Gossypium hirsutum L.)

ZHAO Wen-qing, ZHOU Zhi-guo, MENG Ya-li, CHEN Bing-lin, WANG You-hua

2013, 12 (1): 67-79. DOI: 10.1016/S2095-3119(13)60206-3

Abstract （1483） PDF in ScienceDirect

Crop performance is determined by the combined effects of the genotype of the crop and the environmental conditions of the production system. This study was undertaken to develop a dynamic model for simulating environmental (temperature and solar radiation) and N supply effects on fiber fineness, maturity and micronaire. Three different experiments involving genotypes, sowing dates, and N fertilization rates were conducted to support model development and model evaluation. The growth and development duration of fiber fineness, maturity, and micronaire were scaled by using physiological development time of secondary wall synthesis (PDTSWSP), which was determined based on the constant ratio of SWSP/ BMP. PTP (product of relative thermal effectiveness (RTE) and photosynthetically active radiation (PAR), MJ m-2) and subtending leaf N content per unit area (NA, g m-2) and critical subtending leaf N content per unit area (CNA, g m-2) of cotton boll were calculated or simulated to evaluate effects of temperature and radiation, and N supply. Besides, the interactions among temperature, radiation and N supply were also explained by piecewise function. The overall performance of the model was calibrated and validated with independent data sets from three field experiments with two sowing dates, three or five flowering dates and three or four N fertilization rates for three subsequent years (2005, 2007, and 2009) at three ecological locations. The average RMSE and RE for fiber fineness, maturity, and micronaire predictions were 372 m g-1 and 5.0%, 0.11 m g-1 and 11.4%, 0.3 m g-1 and 12.3%, respectively, indicating a good fit between the simulated and observed data. It appears that the model can give a reliable prediction for fiber fineness, maturity and micronaire formation under various growing conditions.

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Relationship Between the N Concentration of the Leaf Subtending Boll and the Cotton Fiber Quality

WANG You-hua, ZHAO Xin-hua, CHEN Bing-lin, GAO Xiang-bin , ZHOU Zhi-guo

2012, 12 (12): 2013-2019. DOI: 10.1016/S1671-2927(00)8738

Abstract （1089） PDF in ScienceDirect

This experiments were conducted in Nanjing (118º50´E, 32º02´N) and Xuzhou (117°11´E, 34°15´N), Jiangsu Province, China, to study the response of fiber quality to the N concentration of the leaf subtending boll in cotton (Gossypium hirsutum L.). Results suggested that the N dilution curve of the leaf subtending boll can accurately indicate the stagespecific plant N status for fiber development. Fiber strength is likely to be the most variable fiber quality index responding to the leaf N variation which is different in cultivars. Fiber length was the most stable index among strength, length, micronaire, and elongation. There existed an optimum leaf N concentration for fiber strength development in each stage. The optimum leaf N regression curve was very close to the N dilution curve in the middle positional fruiting branches under the 240 kg N ha-1 soil N application rate.

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