|
|
|
Interactions of water and nitrogen addition on soil microbial community composition and functional diversity depending on the inter-annual precipitation in a Chinese steppe |
SUN Liang-jie, QI Yu-chun, DONG Yun-she, HE Ya-ting, PENG Qin, LIU Xin-chao, JIA Jun-qiang, GUO Shu-fang, CAO Cong-cong |
1、Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, P.R.China
2、University of Chinese Academy of Sciences, Beijing 100049, P.R.China
3、Key Laboratory of Crop Nutrition and Fertilization, Ministry of Agriculture/Institute of Agricultural Resources and RegionalPlanning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
|
|
摘要 Water and nitrogen are primary limiting factors in semiarid grassland ecosystems. Our knowledge is still poor regarding the interactive effects of water and N addition on soil microbial communities, although this information is crucial to reveal the mechanisms of the terrestrial ecosystem response to global changes. We addressed this problem by conducting a field experiment with a 15% surplus of the average rainfall under three levels of N addition (50, 100, and 200 kg N ha–1 yr–1) in two consecutive years in Inner Mongolia, China. Microbial community composition and functional diversity were analyzed based on phospholipid fatty acids (PLFA) and BIOLOG techniques, respectively. The results showed that water addition did not affect the soil microbial community composition, but much more yearly precipitation generally decreased the PLFA concentration, which implied a fast response of soil microbes to changes of water condition. Soil fungi was depressed only by N addition at the high level (200 kg N ha–1 yr–1) and without hydrologic leaching, while Gram-negative bacteria was suppressed probably by plant competition at high level N addition but with hydrologic leaching. The study found unilateral positive/negative interactions between water and N addition in affecting soil microbial community, however, climate condition (precipitation) could be a significant factor in disturbing the interactions. This study highlighted that: (1) The sustained effect of pulsed water addition was minimal on the soil microbial community composition but significant on the microbial community functional diversity and (2) the complex interaction between water and N addition on soil microbial community related to the inter-annual variation of the climate and plant response.
Abstract Water and nitrogen are primary limiting factors in semiarid grassland ecosystems. Our knowledge is still poor regarding the interactive effects of water and N addition on soil microbial communities, although this information is crucial to reveal the mechanisms of the terrestrial ecosystem response to global changes. We addressed this problem by conducting a field experiment with a 15% surplus of the average rainfall under three levels of N addition (50, 100, and 200 kg N ha–1 yr–1) in two consecutive years in Inner Mongolia, China. Microbial community composition and functional diversity were analyzed based on phospholipid fatty acids (PLFA) and BIOLOG techniques, respectively. The results showed that water addition did not affect the soil microbial community composition, but much more yearly precipitation generally decreased the PLFA concentration, which implied a fast response of soil microbes to changes of water condition. Soil fungi was depressed only by N addition at the high level (200 kg N ha–1 yr–1) and without hydrologic leaching, while Gram-negative bacteria was suppressed probably by plant competition at high level N addition but with hydrologic leaching. The study found unilateral positive/negative interactions between water and N addition in affecting soil microbial community, however, climate condition (precipitation) could be a significant factor in disturbing the interactions. This study highlighted that: (1) The sustained effect of pulsed water addition was minimal on the soil microbial community composition but significant on the microbial community functional diversity and (2) the complex interaction between water and N addition on soil microbial community related to the inter-annual variation of the climate and plant response.
|
Received: 20 February 2014
Accepted:
|
Fund: This work was financially supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-EW-302), the National Natural Science Foundation of China (41330528, 41373084 and 41203054), and the Special Fund for Agro-Scientific Research in the Public Interest, China (201203012). |
Corresponding Authors:
DONG Yun-she, Tel: +86-10-64856500,E-mail: dongys@igsnrr.ac.cn
E-mail: dongys@igsnrr.ac.cn
|
About author: SUN Liang-jie, E-mail: sunlj-2007@hotmail.com; |
Cite this article:
SUN Liang-jie, QI Yu-chun, DONG Yun-she, HE Ya-ting, PENG Qin, LIU Xin-chao, JIA Jun-qiang, GUO Shu-fang, CAO Cong-cong.
2015.
Interactions of water and nitrogen addition on soil microbial community composition and functional diversity depending on the inter-annual precipitation in a Chinese steppe. Journal of Integrative Agriculture, 14(4): 788-799.
|
Achermann B, Bobbink R. 2003. Empirical Critical Loads forNitrogen. Swiss agency for Environment, Forests andLandscape, Berlin.Albrizio R, Todorovic M, Matic T, Stellacci A M. 2010. Comparingthe interactive effects of water and nitrogen on durum wheatand barley grown in a Mediterranean environment. FieldCrops Research, 115, 179-190Bardgett R D, Freeman C, Ostle N J 2008 Microbialcontributions to climate change through carbon cyclefeedbacks. The ISME Journal, 2, 805-814Bell C W, Tissue D T, Loik M E, Wallenstein M D, Acosta-Martinez V, Erickson R A, Zak J C. 2014. Soil microbialand nutrient responses to 7 years of seasonally alteredprecipitation in a Chihuahuan Desert grassland. GlobalChange Biology, 20, 1657-1673Bi J, Zhang N, Liang Y, Yang H, Ma K. 2012. Interactive effectsof water and nitrogen addition on soil microbial communitiesin a semiarid steppe. Journal of Plant Ecology, 5, 320-329Budge K, Leifeld J, Egli M, Fuhrer J. 2011. Soil microbialcommunities in (sub)alpine grasslands indicate a moderate shift towards new environmental conditions 11 years aftersoil translocation. Soil Biology and Biochemistry, 43,1148-1154Chen S, Lin G, Huang J, Jenerette G D. 2009. Dependenceof carbon sequestration on the differential responses ofecosystem photosynthesis and respiration to rain pulses ina semiarid steppe. Global Change Biology, 15, 2450-2461Cusack D F, Silver W L, Torn M S, Burton S D, Firestone M K.2011. Changes in microbial community characteristics andsoil organic matter with nitrogen additions in two tropicalforests. Ecology, 92, 621-632Demoling F, Nilsson L O, Bååth E. 2008. Bacterial and fungalresponse to nitrogen fertilization in three coniferous forestsoils. Soil Biology and Biochemistry, 40, 370-379Dise N B, Stevens J. 2005. Nitrogen deposition and reduction ofterrestrial biodiversity: evidence from temperate grasslands.Science in China (Series C: Life Sciences), 48, 720-728Docherty K M, Balser T C, Bohannan B J, Gutknecht J L.2012. Soil microbial responses to fire and interactingglobal change factors in a California annual grassland.Biogeochemistry, 109, 63-83Donnison L M, Griffith G S, Bardgett R D. 2000. Determinantsof fungal growth and activity in botanically diversehaymeadows: effects of litter type and fertilizer additions.Soil Biology and Biochemistry, 32, 289-294Dore M H. 2005. Climate change and changes in globalprecipitation patterns: What do we know? EnvironmentInternational, 31, 1167-1181Drenovsky R, Vo D, Graham K, Scow K. 2004. Soil water contentand organic carbon availability are major determinants ofsoil microbial community composition. Microbial Ecology,48, 424-430Drijber R A, Doran J W, Parkhurst A M, Lyon D. 2000. Changesin soil microbial community structure with tillage underlong-term wheat-fallow management. Soil Biology andBiochemistry, 32, 1419-1430Feng L, Zhou T, Wu B, Li T, Luo J. 2011. Projection of futureprecipitation change over China with a high-resolution globalatmospheric model. Advances in Atmospheric Sciences,28, 464-476Fenn M E, Haeuber R, Tonnesen G S, Baron J S, Grossman-Clarke S, Hope D, Jaffe D A, Copeland S, Geiser L, RuethH M. 2003. Nitrogen emissions, deposition, and monitoringin the western United States. BioScience, 53, 391-403Fierer N, Schimel J, Holden P. 2003. Influence of dryingrewettingfrequency on soil bacterial community structure.Microbial Ecology, 45, 63-71Frey S D, Knorr M, Parrent J L, Simpson R T. 2004. Chronicnitrogen enrichment affects the structure and function of thesoil microbial community in temperate hardwood and pineforests. Forest Ecology and Management, 196, 159-171Frostegård Å, Bååth E. 1996. The use of phospholipid fatty acidanalysis to estimate bacterial and fungal biomass in soil.Biology and Fertility of Soils, 22, 59-65Frostegård Å, Tunlid A, Bååth E. 1991. Microbial biomassmeasured as total lipid phosphate in soils of different organiccontent. Journal of Microbiological Methods, 14, 151-163Frostegård Å, Tunlid A, Bååth E. 1993. Phospholipid fatty acidcomposition, biomass, and activity of microbial communitiesfrom two soil types experimentally exposed to differentheavy metals. Applied and Environmental Microbiology,59, 3605-3617Galloway J N, Dentener F J, Capone D G, Boyer E W, HowarthR W, Seitzinger S P, Asner G P, Cleveland C, Green P,Holland E. 2004. Nitrogen cycles: past, present, and future.Biogeochemistry, 70, 153-226Galloway J N, Townsend A R, Erisman J W, Bekunda M, CaiZ, Freney J R, Martinelli L A, Seitzinger S P, Sutton M A.2008. Transformation of the nitrogen cycle: recent trends,questions, and potential solutions. Science, 320, 889-892Grayston S, Campbell C, Bardgett R, Mawdsley J, Clegg C,Ritz K, Griffiths B, Rodwell J, Edwards S, Davies W. 2004.Assessing shifts in microbial community structure across arange of grasslands of differing management intensity usingCLPP, PLFA and community DNA techniques. Applied SoilEcology, 25, 63-84Gruber N, Galloway J N. 2008. An earth-system perspective ofthe global nitrogen cycle. Nature, 451, 293-296Gutknecht J L M, Field C B, Balser T C. 2012. Microbialcommunities and their responses to simulated globalchange fluctuate greatly over multiple years. Global ChangeBiology, 18, 2256-2269Harpole W S, Potts D L, Suding K N. 2007. Ecosystemresponses to water and nitrogen amendment in a Californiagrassland. Global Change Biology, 13, 2341-2348Henriksen T, Breland T. 1999. Nitrogen availability effects oncarbon mineralization, fungal and bacterial growth, andenzyme activities during decomposition of wheat straw insoil. Soil Biology and Biochemistry, 31, 1121-1134Hill G, Mitkowski N, Aldrich-Wolfe L, Emele L, Jurkonie D,Ficke A, Maldonado-Ramirez S, Lynch S, Nelson E. 2000.Methods for assessing the composition and diversity of soilmicrobial communities. Applied Soil Ecology, 15, 25-36Hobbie S E, Eddy W C, Buyarski C R, Adair E C, Ogdahl ML, Weisenhorn P. 2012. Response of decomposing litterand its microbial community to multiple forms of nitrogenenrichment. Ecological Monographs, 82, 389-405Hooper D U, Johnson L. 1999. Nitrogen limitation in drylandecosystems: responses to geographical and temporalvariation in precipitation. Biogeochemistry, 46, 247-293IPCC. 2007. Climate Change 2007: Impacts, Adaptation andVulnerability. Contribution of Working Group II to the FourthAssessment Report of the Intergovernmental PanelonClimate Change, UK.Johnson N C, Rowland D L, Corkidi L, Egerton-Warburton LM, Allen E B. 2003. Nitrogen enrichment alters mycorrhizalallocation at five mesic to semiarid grasslands. Ecology,84, 1895-1908Kandeler E, Mosier A R, Morgan J A, Milchunas D G, King J Y,Rudolph S, Tscherko D. 2008. Transient elevation of carbondioxide modifies the microbial community composition ina semi-arid grassland. Soil Biology and Biochemistry, 40, 162-171Knapp A K, Fay P A, Blair J M, Collins S L, Smith M D, CarlisleJ D, Harper C W, Danner B T, Lett M S, McCarron J K.2002. Rainfall variability, carbon cycling, and plant speciesdiversity in a mesic grassland. Science, 298, 2202-2205Landesman W J, Dighton J. 2011. Shifts in microbial biomassand the bacteria: fungi ratio occur under field conditionswithin 3 h after rainfall. Microbial Ecology, 62, 228-236Li Q, Bai H, Liang W, Xia J, Wan S, van der Putten W H. 2013.Nitrogen addition and warming independently influence thebelowground micro-food web in a temperate steppe. PLOSONE, 8, e60441.Liang C, Jesus E D, Duncan D S, Jackson R D, Tiedje J M,Balser T C. 2012. Soil microbial communities under modelbiofuel cropping systems in southern Wisconsin, USA:Impact of crop species and soil properties. Applied SoilEcology, 54, 24-31Lin Y C, Hu Y G, Ren C Z, Guo L C, Wang C L, Jiang Y, WangX J, Phendukani H, Zeng Z H. 2013. Effects of nitrogenapplication on chlorophyll fluorescence parameters andleaf gas exchange in naked oat. Journal of IntegrativeAgriculture, 12, 2164-2171Liu X, Duan L, Mo J, Du E, Shen J, Lu X, Zhang Y, Zhou X, HeC, Zhang F. 2011. Nitrogen deposition and its ecologicalimpact in China: an overview. Environmental Pollution,159, 2251-2264Liu Y, Li X, Zhang Q, Guo Y, Gao G, Wang J. 2010. Simulation ofregional temperature and precipitation in the past 50 yearsand the next 30 years over China. Quaternary International,212, 57-63Lou Y, Liang W, Xu M, He X, Wang Y, Zhao K. 2011. Strawcoverage alleviates seasonal variability of the topsoilmicrobial biomass and activity. Catena, 86, 117-120Lü F M, Lü X T, Liu W, Han X, Zhang G M, Kong D L, Han XG. 2011. Carbon and nitrogen storage in plant and soil asrelated to nitrogen and water amendment in a temperatesteppe of northern China. Biology and Fertility of Soils, 47,187-196Lü X, Kong D, Pan Q, Simmons M E, Han X. 2012. Nitrogenand water availability interact to affect leaf stoichiometry ina semi-arid grassland. Oecologia, 168, 301-310Lü X T, Wei C Z, Cui Q, Zhang Y H, Han X G. 2010. Interactiveeffects of soil nitrogen and water availability on leaf massloss in a temperate steppe. Plant and Soil, 331, 497-504Macnaughton S J, Jenkins T L, Wimpee M H, Cormiér M R,White D C. 1997. Rapid extraction of lipid biomarkersfrom pure culture and environmental samples usingpressurized accelerated hot solvent extraction. Journal ofMicrobiological Methods, 31, 19-27Manzoni S, Schimel J P, Porporato A. 2012. Responses ofsoil microbial communities to water stress: results from ameta-analysis. Ecology, 93, 930-938Menge D N, Field C B. 2007. Simulated global changes alterphosphorus demand in annual grassland. Global ChangeBiology, 13, 2582-2591Nesci A, Etcheverry M, Magan N. 2004. Osmotic and matricpotential effects on growth, sugar alcohol and sugaraccumulation by Aspergillus section Flavi strains fromArgentina. Journal of Applied Microbiology, 96, 965-972Niu S, Yang H, Zhang Z, Wu M, Lu Q, Li L, Han X, Wan S.2009. Non-additive effects of water and nitrogen additionon ecosystem carbon exchange in a temperate steppe.Ecosystems, 12, 915-926Qi Y, Liu X, Dong Y, Peng Q, He Y, Sun L, Jia J, Cao C.2014. Differential responses of short-term soil respirationdynamics to the experimental addition of nitrogen and waterin the temperate semi-arid steppe of Inner Mongolia, China.Journal of Environmental Sciences, 26, 834-845Rousk J, Brookes P C, Bååth E. 2011. Fungal and bacterialgrowth responses to N fertilization and pH in the 150-year‘Park Grass’ UK grassland experiment. FEMS MicrobiologyEcology, 76, 89-99Schimel J, Balser T C, Wallenstein M. 2007. Microbial stressresponsephysiology and its implications for ecosystemfunction. Ecology, 88, 1386-1394Sheppard L J, Leith I D, Leeson S, Mizunuma T, de Bakker R,Elustondo D, Garcia-Gomez H. 2013. PK additions modifythe effects of N dose and form on species composition,species litter chemistry and peat chemistry in a Scottishpeatland. Biogeochemistry, 116, 39-53Steenwerth K, Jackson L, Calderon F, Scow K, Rolston D. 2005.Response of microbial community composition and activityin agricultural and grassland soils after a simulated rainfall.Soil Biology and Biochemistry, 37, 2249-2262Vestal J R, White D C. 1989. Lipid analysis in microbial ecology.BioScience, 39, 535-541de Vries F T, Bloem J, van Eekeren N, Brusaard L, HofflandE. 2007. Fungal biomass in pastures increases with ageand reduced N input. Soil Biology and Biochemistry, 39,1620-1630de Vries F T, Hoffland E, van Eekeren N, Brussaard L, Bloem J.2006. Fungal/bacterial ratios in grasslands with contrastingnitrogen management. Soil Biology and Biochemistry, 38,2092-2103Wang M, Shi S, Lin F, Hao Z, Jiang P, Dai G. 2012. Effects of soilwater and nitrogen on growth and photosynthetic responseof manchurian ash (Fraxinus mandshurica) seedlings innortheastern China. PLoS ONE, 7, e30754.Williams M A, Rice C W. 2007. Seven years of enhanced wateravailability influences the physiological, structural, andfunctional attributes of a soil microbial community. AppliedSoil Ecology, 35, 535-545Wu F, Bao W, Zhou Z, Li F. 2012. Appropriate nitrogen supplycould improve soil microbial and chemical characteristicswith Sophora davidii seedlings cultivated in water stressconditions. Acta Agriculturae Scandinavica (Section B-Soil& Plant Science), 62, 49-58Xiang S, Doyle A, Holden P A, Schimel J P. 2008. Drying andrewetting effects on C and N mineralization and microbialactivity in surface and subsurface California grassland soils.Soil Biology and Biochemistry, 40, 2281-2289Yevdokimov I V, Gattinger A, Buegger F, Schloter M, Munch J C. 2012. Changa in the structure and activity of a soil microbialcommunity caused by inorganic nitrogen fertilization.Microbiology, 81, 743-749Zak D R, Ringelberg D B, Pregitzer K S, Randlett D L, WhiteD C, Curtis P S. 1996. Soil microbial communities beneathPopulus grandidentata grown under elevated atmosphericCO2. Ecological Applications, 6, 257-262Zak D R, Tilman D, Parmenter R R, Rice C W, Fisher F M,Vose J, Milchunas D, Martin C W. 1994. Plant productionand soil microorganisms in late-successional ecosystems:a continental-scale study. Ecology, 75, 2333-2347Zelles L. 1999. Fatty acid patterns of phospholipids andlipopolysaccharides in the characterisation of microbialcommunities in soil: a review. Biology and Fertility of Soils,29, 111-129Zhang C, Li S, Zhang L, Xin X, Liu X. 2013. Effects of speciesand low sose nitrogen addition on litter decomposition ofthree dominant grasses in Hulun Buir Meadow Steppe.Journal of Resources and Ecology, 4, 20-26Zhang N, Xu W, Yu X, Lin D, Wan S, Ma K. 2013. Impact oftopography, annual burning, and nitrogen addition on soilmicrobial communities in a semiarid grassland. Soil ScienceSociety of America Journal, 77, 1214-1224Zhang Y, Dore A, Liu X, Zhang F. 2011. Simulation of nitrogendeposition in the North China Plain by the FRAME model.Biogeosciences, 8, 3319-3329 |
No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|