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| Comparative genetic mapping revealed powdery mildew resistance gene MlWE4 derived from wild emmer is located in same genomic region of Pm36 and Ml3D232 on chromosome 5BL |
| ZHANG Dong, OUYANG Shu-hong, WANG Li-li, CUI Yu, WU Qiu-hong, LIANG Yong, WANG Zhen-zhong, XIE Jing-zhong, ZHANG De-yun, WANG Yong, CHEN Yong-xing, LIU Zhi-yong |
| State Key Laboratory for Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, P.R.China |
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摘要 Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most devastating wheat diseases. Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is a promising source of disease resistance for wheat. A powdery mildew resistance gene conferring resistance to B. graminis f. sp. tritici isolate E09, originating from wild emmer wheat, has been transferred into the hexaploid wheat line WE4 through crossing and backcrossing. Genetic analyses indicated that the powdery mildew resistance was controlled by a single dominant gene, temporarily designated MlWE4. By mean of comparative genomics and bulked segregant analysis, a genetic linkage map of MlWE4 was constructed, and MlWE4 was mapped on the distal region of chromosome arm 5BL. Comparative genetic linkage maps showed that genes MlWE4, Pm36 and Ml3D232 were co-segregated with markers XBD37670 and XBD37680, indicating they are likely the same gene or alleles in the same locus. The co-segregated markers provide a starting point for chromosome landing and map-based cloning of MlWE4, Pm36 and Ml3D232.
Abstract Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most devastating wheat diseases. Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is a promising source of disease resistance for wheat. A powdery mildew resistance gene conferring resistance to B. graminis f. sp. tritici isolate E09, originating from wild emmer wheat, has been transferred into the hexaploid wheat line WE4 through crossing and backcrossing. Genetic analyses indicated that the powdery mildew resistance was controlled by a single dominant gene, temporarily designated MlWE4. By mean of comparative genomics and bulked segregant analysis, a genetic linkage map of MlWE4 was constructed, and MlWE4 was mapped on the distal region of chromosome arm 5BL. Comparative genetic linkage maps showed that genes MlWE4, Pm36 and Ml3D232 were co-segregated with markers XBD37670 and XBD37680, indicating they are likely the same gene or alleles in the same locus. The co-segregated markers provide a starting point for chromosome landing and map-based cloning of MlWE4, Pm36 and Ml3D232.
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Received: 26 February 2014
Accepted:
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| Fund: This work was financially supported by the National High- Tech R&D Program of China (2011AA100104), the National Basic Research Program of China (2013CB127705), the National Natural Science Foundation of China (31030056, 31210103902), and the Introducing Talents of Disciplines to Universities, Ministry of Education (MOE) of China (111-02-3). |
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Corresponding Authors:
LIU Zhi-yong, Tel: +86-10-62731211,E-mail: zhiyongliu@cau.edu.cn
E-mail: zhiyongliu@cau.edu.cn
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Cite this article:
ZHANG Dong, OUYANG Shu-hong, WANG Li-li, CUI Yu, WU Qiu-hong, LIANG Yong, WANG Zhen-zhong, XIE Jing-zhong, ZHANG De-yun, WANG Yong, CHEN Yong-xing, LIU Zhi-yong.
2015.
Comparative genetic mapping revealed powdery mildew resistance gene MlWE4 derived from wild emmer is located in same genomic region of Pm36 and Ml3D232 on chromosome 5BL. Journal of Integrative Agriculture, 14(4): 603-609.
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| Allen G C, Flores-Vergara M A, Krasynanski S, Kumar S. 2006.A modified protocol for rapid DNA isolation from planttissues using cetyltrimethylammonium bromide. NatureProtocols, 1, 2320-2325Ben-David R, Xie W, Peleg Z, Saranga Y, Dinoor A, FahimaT. 2010. Identification and mapping of PmG16, a powderymildew resistance gene derived from wild emmer wheat.Theoretical and Applied Genetics, 121, 499-510Blanco A, Gadaleta A, Cenci A, Carluccio A V, Abdelbacki A M,Simeone R. 2008. Molecular mapping of the novel powderymildew resistance gene Pm36 introgressed from Triticumturgidum var. dicoccoides in durum wheat. Theoretical andApplied Genetics, 117, 135-142Brenchley R, Spannagl M, Pfeifer M, Barker G L, D’Amore R,Allen A M, McKenzie N, Kramer M, Kerhornou A, BolserD, Kay S, Waite D, Trick M, Bancroft I, Gu Y, Huo N, LuoM C, Sehgal S, Gill B, Kianian S, et al. 2012. Analysis ofthe bread wheat genome using whole-genome shotgunsequencing. Nature, 491, 705-710Devos K M, Gale M D. 1997. Comparative genetics in thegrasses. Plant Molecular Biology Reporter, 35, 3-15Faricelli M E, Valarik M, Dubcovsky J. 2010. Control of floweringtime and spike development in cereals: the earliness per seEps-1 region in wheat, rice, and Brachypodium. Functional& Integrative Genomics, 10, 293-306Faris J D, Zhang Z C, Lu H J, Lu S, Reddy L, Cloutier S, FellersJ P, Meinhardt S W, Rasmussen J B, Xu S S, Oliver R P,Simons K J, Friesen T L. 2010. A unique wheat diseaseresistance-like gene governs effector-triggered susceptibilityto necrotrophic pathogens. Proceedings of the NationalAcademy of Sciences of the United States of America,107, 13544-13549Hua W, Liu Z J, Zhu J, Xie C J, Yang T M, Zhou Y L, DuanX Y, Sun Q X, Liu Z Y. 2009. Identification and geneticmapping of pm42, a new recessive wheat powdery mildewresistance gene derived from wild emmer (Triticum turgidumvar. dicoccoides). Theoretical and Applied Genetics, 119,223-230International Brachypodium Initiative. 2010. Genomesequencing and analysis of the model grass Brachypodiumdistachyon. Nature, 463, 763-768International Rice Genome Sequencing Project. 2005. The mapbasedsequence of the rice genome. Nature, 436, 793-800Ji X L, Xie C J, Ni Z F, Yang T M, Nevo E, Fahima T, Liu ZY, Sun Q X. 2008. Identification and genetic mapping of apowdery mildew resistance gene in wild emmer (Triticumdicoccoides) accession IW72 from Israel. Euphytica, 159,385-390Jia J, Zhao S, Kong X, Li Y, Zhao G, He W, Appels R, PfeiferM, Tao Y, Zhang X, Jing R, Zhang C, Ma Y, Gao L, GaoC, Spannagl M, Mayer K F, Li D, Pan S, Zheng F, et al.2013. Aegilops tauschii draft genome sequence reveals agene repertoire for wheat adaptation. Nature, 496, 91-95Lander E S, Green P, Abrahamson J, Barlow A, Daly M J,Lincoln S E, Newberg L A. 1987. MAPMAKER: an interactivecomputer package for constructing primary genetic linkagemaps of experimental and natural populations. Genomics, 1, 174-181Li G Q, Fang T L, Zhang H T, Xie C J, Li H, Yang T M, Nevo E,Fahima T, Sun Q X, Liu Z Y. 2009. Molecular identificationof a new powdery mildew resistance gene Pm41 onchromosome 3BL derived from wild emmer (Triticumturgidum var. dicoccoides). Theoretical and AppliedGenetics, 119, 531-539Ling H Q, Zhao S, Liu D, Wang J, Sun H, Zhang C, Fan H, Li D,Dong L, Tao Y, Gao C, Wu H, Li Y, Cui Y, Guo X, Zheng S,Wang B, Yu K, Liang Q, Yang W, et al. 2013. Draft genomeof the wheat A-genome progenitor Triticum urartu. Nature,496, 87-90Liu Z J, Zhu J, Cui Y, Liang Y, Wu H, Song W, Liu Q, Yang TM, Sun Q X, Liu Z Y. 2012. Identification and comparativemapping of a powdery mildew resistance gene derivedfrom wild emmer (Triticum turgidum var. dicoccoides) onchromosome 2BS. Theoretical and Applied Genetics, 124,1041-1049Liu Z Y, Sun Q X, Ni Z F, Nevo E, Yang T M. 2002. Molecularcharacterization of a novel powdery mildew resistance genePm30 in wheat originating from wild emmer. Euphytica,123, 21-29Lu H J, Faris J D. 2006. Macro- and microcolinearity betweenthe genomic region of wheat chromosome 5B containing theTsn1 gene and the rice genome. Functional & IntegrativeGenomics, 6, 90-103McIntosh R A, Yamazaki Y, Dubcovsky J, Rogers J, MorrisC, Appels R, Xia X C. 2013. Catalogue of gene symbolsfor wheat. In: Proceedings of the 12th International WheatGenetics Symposium. Yokohama, Japan.Michelmore R W, Paran I, Kesseli R V. 1991. Identificationof markers linked to disease-resistance genes by BulkedSegregant Analysis - A rapid method to detect markers inspecific genomic regions by using segregating populations.Proceedings of the National Academy of Sciences of theUnited States of America, 88, 9828-9832Mohler V, Zeller F J, Wenzel G, Hsam L K. 2005. Chromosomallocation of genes for resistance to powdery mildew incommon wheat (Triticum aestivum L. em Thell.). 9. GeneMlZec1 from the Triticum dicoccoides-derived wheat lineZecoi-1. Euphytica, 142, 161-167Paterson A H, Bowers J E, Bruggmann R, Dubchak I, GrimwoodJ, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A,Schmutz J, Spannagl M, Tang H, Wang X, Wicker T, BhartiA K, Chapman J, Feltus F A, Gowik U, Grigoriev I V, et al.2009. The Sorghum bicolor genome and the diversificationof grasses. Nature, 457, 551-556Reader S M, Miller T E. 1991. The introduction into bread wheatof a major gene for resistance to powdery mildew from wildemmer wheat. Euphytica, 53, 57-60Rong J K, Millet E, Manisterski J, Feldman M. 2000. A newpowdery mildew resistance gene: Introgression from wildemmer into common wheat and RFLP-based mapping.Euphytica, 115, 121-126Schnable P S, Ware D, Fulton R S, Stein J C, Wei F, PasternakS, Liang C, Zhang J, Fulton L, Graves T A, Minx P, ReilyA D, Courtney L, Kruchowski S S, Tomlinson C, StrongC, Delehaunty K, Fronick C, Courtney B, Rock S M, et al.2009. The B73 maize genome: complexity, diversity, anddynamics. Science, 326, 1112-1115Spielmeyer W, Singh R P, McFadden H, Wellings C R, Huerta-Espino J. 2008. Fine scale genetic and physical mappingusing interstitial deletion mutants of Lr34/Yr18: a diseaseresistance locus effective against multiple pathogens inwheat. Theoretical and Applied Genetics, 116, 481-490Wiebe K, Harris N S, Faris J D, Clarke J M, Knox R E, Taylor GJ, Pozniak C J. 2010. Targeted mapping of Cdu1, a majorlocus regulating grain cadmium concentration in durumwheat (Triticum turgidum L. var. durum). Theoretical andApplied Genetics, 121, 1047-1058Xie W, Ben-David R, Zeng B, Distelfeld A, Roder M S, DinoorA, Fahima T. 2012. Identification and characterization of anovel powdery mildew resistance gene PmG3M derivedfrom wild emmer wheat, Triticum dicoccoides. Theoreticaland Applied Genetics, 124, 911-922Xue F, Ji W, Wang C, Zhang H, Yang B. 2012. High-densitymapping and marker development for the powdery mildewresistance gene PmAS846 derived from wild emmer wheat(Triticum turgidum var. dicoccoides). Theoretical andApplied Genetics, 124, 1549-1560You F M, Huo N, Gu Y Q, Luo M C, Ma Y, Hane D, Lazo GR, Dvorak J, Anderson O D. 2008. BatchPrimer3: a highthroughput web application for PCR and sequencing primerdesign. BMC Bioinformatics, 9, 253.Yu G T, Cai X W, Harris M O, Gu Y Q, Luo M C, Xu S S.2009. Saturation and comparative mapping of the genomicregion harboring Hessian fly resistance gene H26 in wheat.Theoretical and Applied Genetics, 118, 1589-1599Zhang H T, Guan H Y, Li J T, Zhu J, Xie C J, Zhou Y L,Duan X Y, Yang T, Sun Q X, Liu Z Y. 2010. Genetic andcomparative genomics mapping reveals that a powderymildew resistance gene Ml3D232 originating from wildemmer co-segregates with an NBS-LRR analog in commonwheat (Triticum aestivum L.). Theoretical and AppliedGenetics, 121, 1613-1621Zhang W J, Olson E, Saintenac C, Rouse M, Abate Z, Jin Y,Akhunov E, Pumphrey M, Dubcovsky J. 2010. Genetic mapsof stem rust resistance gene Sr35 in diploid and hexaploidwheat. Crop Science, 50, 2464-2474Zhang Z C, Friesen T L, Xu S S, Shi G J, Liu Z H, RasmussenJ B, Faris J D. 2011. Two putatively homoeologous wheatgenes mediate recognition of SnTox3 to confer effectortriggeredsusceptibility to Stagonospora nodorum. The PlantJournal, 65, 27-38Zhou R, Zhu Z, Kong X, Huo N, Tian Q, Li C, Jin P, Dong Y,Jia J. 2005. Development of wheat near-isogenic linesfor powdery mildew resistance. Theoretical and AppliedGenetics, 110, 640-648 |
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