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| Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate |
| Abdelfattah Z M Salem, German Buendía-Rodríguez, Mona M M Elgh, our, María A Mariezcurrena Berasain, Francisco J Peña Jiménez, Alberto B Pliego, Juan C V Chagoyán, María A Cerrillo, Miguel A Rodríguez |
1、Faculty of Veterinary Medicine and Animal Science, Autonomous University of the State of Mexico, Toluca P.O. 50000, Mexico
2、National Center of Disciplinary Research in Animal Physiology and Improvement, INIFAP Ajuchitlán, Querétaro 76280, Mexico
3、Institute of Agricultural Sciences, Autonomous University of Hidalgo, Ex-Hda. of Aquetzalpa, Tulancingo, Hidalgo P.O. 43600,
Mexico
4、Faculty of Veterinary Medicine and Animal Science, Juárez University of the Durango State, Durango, P.O. 34280, Mexico |
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摘要 An in vitro gas production (GP) technique was used to investigate the effects of combining different doses of Salix babylonica extract (SB) with exogenous fibrolytic enzymes (EZ) based on xylanase (X) and cellulase (C), or their mixture (XC; 1:1 v/v) on in vitro fermentation characteristics of a total mixed ration of corn silage and concentrate mixture (50:50, w/w) as substrate. Four levels of SB (0, 0.6, 1.2 and 1.8 mL g–1 dry matter (DM)) and four supplemental styles of EZ (1 μL g–1 DM; control (no enzymes), X, C and XC (1:1, v/v) were used in a 4×4 factorial arrangement. In vitro GP (mL g–1 DM) were recorded at 2, 4, 6, 8, 10, 12, 24, 36, 48 and 72 h of incubation. After 72 h, the incubation process was stopped and supernatant pH was determined, and then filtered to determine dry matter degradability (DMD). Fermentation parameters, such as the 24 h gas yield (GY24), in vitro organic matter digestibility (OMD), metabolizable energy (ME), short chain fatty acid concentrations (SCFA), and microbial crude protein production (MCP) were also estimated. Results indicated that there was a SB´EZ interaction (P<0.0001) for the asymptotic gas production (b), the rate of gas production (c), GP from 6 to 72 h, GP2 (P=0.0095), and GP4 (P=0.02). The SB and different combination of enzymes supplementation influenced (P<0.001) in vitro GP parameters after 12 h of incubation; the highest doses of SB (i.e., 1.8 mL g–1 DM), in the absence of any EZ, quadratically increased (P<0.05) the initial delay before GP begins (L) and GP at different incubation times, with lowering b (quadratic effect, P<0.0001) and c (quadratic effect, P<0.0001; linear effect, P=0.0018). The GP was the lowest (P<0.05) when the highest SB level was combined with cellulose. There were SB´EZ interactions (P<0.001) for OMD, ME, the partitioning factor at 72 h of incubation (PF72), GY24, SCFA, MCP (P=0.0143), and pH (P=0.0008). The OMD, ME, GY24 and SCFA with supplementation of SB extract at 1.8 mL g–1 DM were higher (P<0.001) than the other treatments, however,PF72 was lower (quadratic effect, P=0.0194) than the other levels. Both C and X had no effect (P>0.05) on OMD, pH, ME, GY24, SCFA and MP. The combination of SB with EZ increased (P<0.001) OMD, ME, SCFA, PF72 and GP24, whereas there was no impact on pH. It could be concluded that addition of SB extract, C, and X effectively improved the in vitro rumen fermentation, and the combination of enzyme with SB extract at the level of 1.2 mL g–1 was more effective than the other treatments.
Abstract An in vitro gas production (GP) technique was used to investigate the effects of combining different doses of Salix babylonica extract (SB) with exogenous fibrolytic enzymes (EZ) based on xylanase (X) and cellulase (C), or their mixture (XC; 1:1 v/v) on in vitro fermentation characteristics of a total mixed ration of corn silage and concentrate mixture (50:50, w/w) as substrate. Four levels of SB (0, 0.6, 1.2 and 1.8 mL g–1 dry matter (DM)) and four supplemental styles of EZ (1 μL g–1 DM; control (no enzymes), X, C and XC (1:1, v/v) were used in a 4×4 factorial arrangement. In vitro GP (mL g–1 DM) were recorded at 2, 4, 6, 8, 10, 12, 24, 36, 48 and 72 h of incubation. After 72 h, the incubation process was stopped and supernatant pH was determined, and then filtered to determine dry matter degradability (DMD). Fermentation parameters, such as the 24 h gas yield (GY24), in vitro organic matter digestibility (OMD), metabolizable energy (ME), short chain fatty acid concentrations (SCFA), and microbial crude protein production (MCP) were also estimated. Results indicated that there was a SB´EZ interaction (P<0.0001) for the asymptotic gas production (b), the rate of gas production (c), GP from 6 to 72 h, GP2 (P=0.0095), and GP4 (P=0.02). The SB and different combination of enzymes supplementation influenced (P<0.001) in vitro GP parameters after 12 h of incubation; the highest doses of SB (i.e., 1.8 mL g–1 DM), in the absence of any EZ, quadratically increased (P<0.05) the initial delay before GP begins (L) and GP at different incubation times, with lowering b (quadratic effect, P<0.0001) and c (quadratic effect, P<0.0001; linear effect, P=0.0018). The GP was the lowest (P<0.05) when the highest SB level was combined with cellulose. There were SB´EZ interactions (P<0.001) for OMD, ME, the partitioning factor at 72 h of incubation (PF72), GY24, SCFA, MCP (P=0.0143), and pH (P=0.0008). The OMD, ME, GY24 and SCFA with supplementation of SB extract at 1.8 mL g–1 DM were higher (P<0.001) than the other treatments, however,PF72 was lower (quadratic effect, P=0.0194) than the other levels. Both C and X had no effect (P>0.05) on OMD, pH, ME, GY24, SCFA and MP. The combination of SB with EZ increased (P<0.001) OMD, ME, SCFA, PF72 and GP24, whereas there was no impact on pH. It could be concluded that addition of SB extract, C, and X effectively improved the in vitro rumen fermentation, and the combination of enzyme with SB extract at the level of 1.2 mL g–1 was more effective than the other treatments.
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Received: 04 November 2013
Accepted:
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| Fund: the financial support from the IAEA, Vienna, Austria, Research Contract number MEX16307 within the D3.10.27 Coordinated Research Project. |
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Corresponding Authors:
Abdelfattah Z M Salem, Tel/Fax: +1-521-7162695171, E-mail: asalem70@yahoo.com
E-mail: asalem70@yahoo.com
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Cite this article:
Abdelfattah Z M Salem, German Buendía-Rodríguez, Mona M M Elgh, our , María A Mariezcurrena Berasain, Francisco J Pe?a Jiménez, Alberto B Pliego, Juan C V Chagoyán, María A Cerrillo, Miguel A Rodríguez.
2015.
Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate. Journal of Integrative Agriculture, 14(1): 131-139.
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| AOAC (Association of Official Analytical Chemists). 1997.Official Methods of Analysis. 16th ed. Association of OfficialAnalytical Chemists, Arlington, Virginia, USA.Baah J, Shelford J A, Hristov A N, McAllister T A, Cheng KJ. 2005. Effects of Tween80 and fibrolytic enzymes onruminal fermentation and digestibility of feeds in Holsteincows. Asian-Australasian Journal of Animal Sciences, 18,816–824.Beauchemin K A, Colombatto D, Morgavi D P, Yang W Z, RodeL M. 2004. Mode of action of exogenous cell wall degradingenzymes for ruminants. Canadian Journal of AnimalScience, 84, 13–22.Beauchemin K A, Colombatto D, Morgavi D P, Yang W Z.2003. Use of fibrolytic enzymes to improve feed utilizationby ruminants. Journal of Animal Science, 81, E37–E47.Beauchemin K A, Yang W Z, Rode L M. 1999. Effects of grainsource and enzyme additive on site and extent of nutrientdigestion in dairy cows. Journal of Dairy Science, 82,378–390.Bhat M K, Hazlewood G P. 2001. Enzymology and othercharacteristics of cellulases and xylanases. In: Bedford MR, Partridge G G, eds., Enzymes Farm Animal Nutrition.CABI Publishing, Wallingford, UK. pp. 11–60.Blümmel M, Steingass H, Becker K. 1997. The relationshipbetween in vitro gas production, in vitro microbial biomassyield and 15N incorporation and its implications for theprediction of voluntary feed intake of roughages. BritishJournal of Nutrition, 77, 911–921.Busquet M, Calsamiglia S, Ferret A, Kamel C. 2006. Plantextracts affect in vitro rumen microbial fermentation. Journalof Dairy Science, 89, 761–771.Calsamiglia S, Busquet M, Cardozo P W, Castillejos L, FerretA. 2007. Essential oils as modifiers of rumen microbialfermentation. Journal of Dairy Science, 90, 2580–2595.Carro M D, Giraldo L A, Mohamed A H, Ranilla M J, Tejido M L. 2007. In vitro ruminal fermentation of low-quality foragesas influenced by the treatment with exogenous fibrolyticenzymes. In: Priolo A, Biondi L, Ben Salem H, Morand-Fehr P, eds., Advanced Nutrition and Feeding Strategiesto Improve Sheep and Goat. CIHEAM, Zaragoza. pp.263–267.Chen W, Ohmiya K, Shimizu S, Kawakami H. 1988. Isolationand characterization of an anaerobic dehydrodivanillindegradingbacterium. Applied and EnvironmentalMicrobiology, 54, 1254–1257.Colombatto D, Morgavi D P, Furtado A F, Beauchemin K A.2003. Screening of exogenous enzymes for ruminant diets:relationship between biochemical characteristics and invitro ruminal degradation. Journal of Animal Science, 81,2628–2638.Cowan M M. 1999. Plant products as antimicrobial agents.Clinical Microbiology Reviews, 12, 564–582.Eun J S, Beauchemin K A, Schulze H. 2006. Use of in vitrofermentation bioassay to evaluate improvements indegradation of alfalfa hay due to exogenous feed enzymes.Animal Feed Science and Technology, 135, 315–328.France J, Dijkstra J, Dhanoa M S, Lopez S, Bannink A. 2000.Estimating the extent of degradation of ruminant feedsfrom a description of their gas production profiles observedin vitro: Derivation of models and other mathematicalconsiderations. Clinical Microbiology Reviews, 83, 143–150.Gashe B A. 1992. Cellulase production and activity byTrichoderma sp. A-001 Journal of Applied Bacteriology,73, 79–82Getachew G, Makkar H P S, Becker K. 2002. Tropical browses:Contents of phenolic compounds, in vitro gas productionand stoichiometric relationship between short chain fattyacid and in vitro gas production. Journal of AgriculturalScience, 139, 341–352.Giraldo L A, Ranilla M J, Tejido M L, Carro M D. 2004. Effectsof enzyme application method on the in vitro rumenfermentation of tropical forages. Journal of Animal and FeedSciences, 13, 63–66.Goering M K, Van Soest P J. 1970. Forage fiber analysis(apparatus, reagents, procedures and some applications).In: Agriculture Handbook, No. 379. Agricultural ResearchService, USDA, Washington, USA.Jiménez-Peralta F S, Salem A Z M, Mejia-Hernández P,González-Ronquillo M, Albarrán-Portillo P, Rojo-RubioR, Tinoco-Jaramillo J L. 2011. Influence of individual andmixed extracts of two tree species on in vitro gas productionkinetics of high-concentrate diet fed to growing lambs.Livestock Science, 136, 192–200.Khattab H M, Gado H M, Kholif A E, Mansour A M, Kholif AM. 2011. The potential of feeding goats sun dried rumencontents with or without bacterial inoculums as replacementfor berseem clover and the effects on milk production andanimal health. International Journal of Dairy Science, 6,267–277.Khattab H M, Gado H M, Salem A Z M, Camacho L M, El-SayedM M, Kholif A M, El-Shewy A A, Kholif A E. 2013. Chemicalcomposition and in vitro digestibility of Pleurotus ostreatusspent rice straw. Animal Nutrition and Feed Technology,13, 507–516.Kim W, Gamo Y, Yahaya M S, Yimiti W, Goto M. 2005.Pretreatment of surfactant Tween 80 and fibrolytic enzymeinfluencing volatile fatty acid and methane production of atotal mixed ration by mixed rumen microorganisms at invitro. Grassland Science, 51, 145–152.Krueger N, Staples C R, Littell R C, Dean D B, Krueger W,Adesogan A T. 2003. The influence of enzymes containinghigh esterase, cellulose, and endogalacturonase activityon the digestibility of mature, C4 grass hays. Tropical andSubtropical Agroecosystems, 3, 201–204.Kung Jr L, Treacher R J, Nauman G A, Smagala A M, EndresK M, Cohen M A. 2000. The effect of treating forageswith fibrolytic enzymes on its nutritive value and lactationperformance of dairy cows. Journal of Dairy Science, 83,115–122.Lewis G E, Sanchez W K, Hunt C W, Guy M A, Pritchard GT, Swanson B I, Treacher R J. 1999. Effect of direct-fedfibrolytic enzymes on the lactational performance of dairycows. Journal of Dairy Science, 82, 611–617.Makkar H P S, Sen S, Blummel M, Becker K. 1998. Effectsof fractions containing saponins from Yucca schidigera,Quillaja saponaria, and Acacia auriculoformison rumenfermentation. Journal of Agricultural and Food Chemistry,46, 4324–4328.Martinez S, Madrid J, Hernandez F, Megias M D, SotomayorJ A, Jordan M J. 2006. Effect of thyme essential oils(Thymus hyemalis and Thymus zygis) and monensin on invitro ruminal degradation and volatile fatty acid production.Journal of Agricultural and Food Chemistry, 54, 6598–6602.Menke K H, Raab L, Salewski A, Steingass H, Fritz D,Schneider W. 1979. The estimation of the digestibilityand metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubatedwith rumen liquor in vitro. Journal of Agricultural Science(Cambridge), 93, 217–222.Mitsumori M, Sun W. 2008. Control of rumen microbialfermentation for mitigating methane emissions from therumen. Asian-Australasian Journal of Animal Sciences,21, 144–154.Morgavi D P, Beauchemin K A, Nsereko V L, Rode L M, IwaasaA D, Yang W Z, McAllister T A, Wang Y. 2000. Synergybetween ruminal fibrolytic enzymes and enzymes fromTrichoderma longibrachiatum. Journal of Dairy Science,83, 1310–1321.NRC (National Research Council). 2001. Nutrient Requirementsof Dairy Cattle. 7th ed. National Academy of Sciences,National Research Council, Washington, USA.Nsereko V L, Beauchemin K A, Morgavi D P, Rode L M, FurtadoA F, McAllister T A, Iwaasa A D, Yang W Z, Wang Y. 2002.Effect of a fibrolytic enzyme preparation from Trichodermalongibrachiatum on the rumen microbial population of dairycows. Canadian Journal of Microbiology, 48, 14–20.Ørskov E R, McDonald L. 1979. The estimation of protein degradability in the rumen from incubationmeasurements weighted according to the rate of passage.Journal of Agricultural Science (Cambridge), 92, 499–503.Rezaei N, Pour H A. 2012. Evaluation effect of thyme extract ondegradability of soybean meal with gas product technique.Advances in Bioresearch, 3, 45–47.Salem A Z M, López S, Robinson P H. 2012. Plant bioactivecompounds in ruminant agriculture- Impacts andopportunities. Animal Feed Science and Technology, 176,1–4.Salem A Z M, Zhou C S, Tan Z L, Mellado M, Odongo N E,Cipriano-Salazar M, Elghandour M M Y. 2013. In vitrodigestibility, chemical composition, and kinetics of gasproduction of foliage from four fodder trees ensiled withor without molasses and urea. Journal of IntegrativeAgriculture, 12, 1234–1242.SAS Institute. 2002. SAS/STAT User’s Guide. 9th ed. SASInstitute, Cary, North Carolina.van Soest P J. 1994. Nutritional Ecology of the Ruminant.Cornell University Press, Ithaca, NY, USA.van Soest P J, Robertson J B, Lewis B A. 1991. Methodsfor dietary fiber, neutral detergent fiber and non-starchpolysaccharides in relation to animal nutrition. Journal ofDairy Science, 74, 3583–3597.Theodorou M K, Williams B A, Dhanoa M S, McAllan A B,France J. 1994. A simple gas production method using apressure transducer to determine the fermentation kineticsof ruminant feeds. Animal Feed Science and Technology,48, 185–197.Yang W Z, Beauchemin K A, Rode L M. 2000. A comparison ofmethods of adding fibrolytic enzymes to lactating cow diets.Journal of Dairy Science, 83, 2512–2520. |
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