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| The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Ras?n Area of North Korea |
| JIA Li-jun, ZHANG Shou-fa, CAO Shi-nuo, QIAN Nian-chao, YU Long-zheng , XUAN Xue-nan |
1 Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji 133002, P.R.China
2 National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan |
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摘要 The objective of this study was to investigate the epidemiology of bovine Piroplasms infections in the Ras?n area of North Korea. The survey was carried out by light microscopic examination of Giemsa-stained blood smears, PCR, and phylogenetic evolution analysis of 128 blood samples collected from the Ras?n area. The results showed that the infection rates of the small and large parasites were about 2.5 and 1.5% on average, respectively, in all Theileria sergenti and Babesia ovatapositive blood smears by microscopic examination of blood smears. The detection rate of T. sergenti Giemsa-stained smears was 43.75%, while that with PCR was 67.97%. The detection rate of B. ovata Giemsa-stained smears was 49.21%, while that with PCR was 71.88%. The sequence and phylogenetic analysis of DNA showed 98.84% homology between the 18S rRNA gene sequences of T. sergenti isolates from North Korean and that of Yanbian state from China, indicating the closest genetic relationship between both of them. Moreover, 100% homology was shown between the 18S rRNA gene sequence of B. ovata isolates from North Korea and the published sequence AY081192 of GenBank, indicating the closest genetic relationship between both of them. This survey confirmed that Ras?n is the endemic area of T. sergenti and B. ovata in North Korea.
Abstract The objective of this study was to investigate the epidemiology of bovine Piroplasms infections in the Ras?n area of North Korea. The survey was carried out by light microscopic examination of Giemsa-stained blood smears, PCR, and phylogenetic evolution analysis of 128 blood samples collected from the Ras?n area. The results showed that the infection rates of the small and large parasites were about 2.5 and 1.5% on average, respectively, in all Theileria sergenti and Babesia ovatapositive blood smears by microscopic examination of blood smears. The detection rate of T. sergenti Giemsa-stained smears was 43.75%, while that with PCR was 67.97%. The detection rate of B. ovata Giemsa-stained smears was 49.21%, while that with PCR was 71.88%. The sequence and phylogenetic analysis of DNA showed 98.84% homology between the 18S rRNA gene sequences of T. sergenti isolates from North Korean and that of Yanbian state from China, indicating the closest genetic relationship between both of them. Moreover, 100% homology was shown between the 18S rRNA gene sequence of B. ovata isolates from North Korea and the published sequence AY081192 of GenBank, indicating the closest genetic relationship between both of them. This survey confirmed that Ras?n is the endemic area of T. sergenti and B. ovata in North Korea.
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Received: 08 April 2012
Accepted:
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| Fund: National Natural Science Foundation of China (30560112, 30960278), and Natural Science Foundation of Jilin Province of China (201115230). |
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Corresponding Authors:
Correspondence ZHANG Shou-fa, E-mail: shoufazhang@sina.com
E-mail: shoufazhang@sina.com
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| About author: JIA Li-jun, Mobile: 13844705029, E-mail: ljjia@ybu.edu.cn |
Cite this article:
JIA Li-jun, ZHANG Shou-fa, CAO Shi-nuo, QIAN Nian-chao, YU Long-zheng , XUAN Xue-nan.
2013.
The Finding and Phylogenetic Evolution Analysis of Bovine Piroplasms in the Ras?n Area of North Korea. Journal of Integrative Agriculture, 12(10): 1847-1854.
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| [1]Ahmed J S, Rothert M, Steuber S, Schein E. 1989. In vitro proliferative and cytotoxic responses of PBL from Theileria annulata-immune cattle. Zentralbl Veterinarmed (B), 36, 584-592[2]Aktas M, Altay K, Dumanli N A. 2006. Molecular survey of bovine Theileria parasites among apparently healthy cattle and with a note on the distribution of ticks in eastern Turkey. Veterinary Parasitology, 138, 179-185[3]Allsopp M T, Cavalier-Smith T, de Waal D T, Allsopp B A. 1994. Phylogeny and evolution of the piroplasms. Parasitology, 108, 147-152[4]Altangerel K, Sivakumar T, Inpankaew T, Jittapalapong S, Terkawi M, Akio U, Xuan X, Igarashi I, Yokoyama N. 2011. Molecular prevalence of different genotypes of Theileria orientalis detected from cattle and water buffaloes in Thailand. Journal of Parasitology, 97, 1075- 1079. Altangerel K B, Battsetseg B, Battur B, Sivakumar T, Batmagnai E, Javkhlan G, Tuvshintulga B, Igarashi I, Matsumoto K, Inokuma H. 2011. The first survey of Theileria orientalis infection in Mongolian cattle. Veterinary Parasitology, 182, 343-348[5]Aparna M, Ravindran R, Vimalkumar M B, Lakshmanan B, Rameshkumar P, Ajith Kumar K G, Promod K, Ajithkumar S, Ravishankar C, Devada K. 2011. Molecular characterization of Theileria orientalis causing fatal infection in crossbred adult bovines of South India. Parasitology International, 60, 524-529[6]Assadian O, Stanek G. 2002. Theobald Smith - the discoverer of ticks as vectors of disease. Wiener Klinische Wochenschrift, 114, 479-481[7]Baek B K, Kim B S, Lee H I. 1990. Fine structures of Theileria sergenti in Korean native cattle. Korean Journal of Veterinary Research, 30, 465-471[8]Bai Q, Liu G Y, Han G F, Hui Y. 1994. The separation and additional spread experiment of Babesia bovis in Zhangjiachuan, Gansu. Chinese Journal of Veterinary Science and Technology, 24, 9-10 (in Chinese) [9]Bai Q, Liu G Y, Zhang L, Zhou J Y. 1990. The discovery and separation of Babesia ovata in China. Chinese Journal of Veterinary Medicine, 16, 2-4[10](in Chinese) Cassini R, Marcer F, di Regalbono A F, Cancrini G, Gabrielli S, Moretti A, Galuppi R, Tampieri M P, Pietrobelli M. 2012. New insights into the epidemiology of bovine piroplasmoses i n I t a l y . Ve ter inary Parasitology, 184, 77-82[11]Chae J, Lee J, Kwon O, Holman P J, Waghela S D, Wagner G G. 1998. Nucleotide sequence heterogeneity in the small subunit ribosomal RNA gene variable (V4) region among and within geographic isolates of Theileria from cattle, elk and white-tailed deer. Veterinary Parasitology, 75, 41-52[12]Cossio-Bayagur R, Pillars R, Schlater J, Holman P. 2002. Theileria buffeli infection of a Michigan cow confirmed by small subunit ribosomal RNA gene analysis. Veterinary Parasitology, 105, 105-110[13]Ellis J, Hefford C, Baverstock P R, Dalrymple B P, Johnson A M. 1992. Ribosomal DNA sequence of Babesia and Thelleria. Molecular and Biochemical Parasitology, 54, 96-97[14]Ghaemi P, Hoghooghi-Rad N, Shayan P, Eckert B. 2012. Detection of Theileria orientalis in Iran by semi-nested PCR. Parasitology Research, 110, 527-531[15]Govaerts M M, Voet M, Volckaert G, Goddeeris B M. 1998. PCR amplification and sequence of the p33 piroplasm surface antigen gene of a Theileria species isolated from cattle in west Java, Indonesia. Annals of the New York Academy of Sciences, 849, 126-136[16]Higuchi S, Tazawa K, Matuda K, Kawamura S, Yasuda Y. 1989. Morphological observations of Babesia ovata in bovine lymphocyte. The Kitasato Archives of Experimental Medicine, 62, 135-138[17]Islam M K, Jabbar A, Campbell B E, Cantacessi C, Gasser R B. 2011. Bovine theileriosis - an emerging problem in south-eastern Australia. Infection Genetics and Evolution, 11, 2095-2097[18]Jin C, Jia L J, Wang Y, Wang N, Mo T T, Zhang S F. 2011. The comparison of PCR methods of detecting Theileria sergenti based on different target gene. Animal Husbandry and Veterinary Medicine, 43, 1-3 (in Chinese) [19]Jin C M. 2005. Cloning of P33 major surface protein gene of Theileria sergenti and the establishment of PCR detection method. MSc thesis, Yanbian University, China. (in Chinese) [20]Jiang J S. 2000. Animal Protozoosis. China Agricultural University Press, Beijing, China. pp. 88-91 (in Chinese) [21]Jian Z J, Huang J Y, Ma S Z, Yuan J L, Su G C, Miao Z Q, Shen J Y, Zhang L J, Li X J. 2008. Cloning and expression of Tams1 gene from the bovine Theileria annulata in Xinjiang. Chinese Journal of Preventive Veterinary Medicine, 30, 784-789 (in Chinese) [22]Khukhuu A, Lan D T, Long P T, Ueno A, Li Y, Luo Y, Macedo A C, Matsumoto K, Inokuma H, Kawazu S. 2011. Molecular epidemiological survey of Theileria orientalis in Thua Thien Hue Province, Vietnam. Journal of Veterinary Medical Science, 73, 701-705[23]Liu A H, Guan G Q, Liu J L, Li Y Q, Ma M L, Niu Q L, Ren Q Y, Yin H, Luo J X. 2009. Studies on comparison of 18S rRNA gene sequences of Theileria species infective to cattle. Acta Veterinaria et Zootechnica Sinica, 40, 1063-1068 (in Chinese) [24]Liu J L. 2007. Sequencing and Application of Ribosomal RNA Intenal Transcribed Spacer of Bovine Piroplasma. Chinese Academy of Agricultural Sciences, China. (in Chinese) [25]Luo J X, Yin H, Guan G, Yang D, Liu A H, Ma M, Liu Z J, Dang Z S, Liu G Y, Bai Q A. 2005a. A comparison of small-subunit ribosomal RNA gene sequences of bovine Babesia species transmited by Haemaphysalis spp. in China. Parasitology Research, 95, 145-149[26]Luo J X, Yin H, Liu G Y, Guan G Q, Liu Z J, Liu A H, Dang Z S, Ma M L, Lu B Y, Sun C Q. 2005b. The comparison of 18S rRNA gene sequence in bovine Babesia. Acta Veterinaria et Zootechnica Sinica, 36, 906- 911. (in Chinese) [27]Ma T, Zhou J L. 2006. Advances in molecular biology of piroplasmosis. Chinese Journal of Veterinary Parasitology, 14, 22-27 (in Chinese) [28]Minami T, Fujinaga T, Furuya K, Ishihara T. 1980. Clinicohematologic and serological comparison of Japanese and Russian strains of Theileria sergenti. National Institute of Animal Health Quarterly, 20, 44-52[29]Wagland B M, Roberts J A, Sutherst R W. 1979. Growth of Haemaphysalis (Kaiseriana) longicornis on cattle. International Journal for Parasitology, 9, 177-182[30]Weber G. 1980. Ultrastructure and cytochemistry of the pellicle and apical complexes of the kinete of Babesia bigemina and Babesia ovis in the hemolymph and oavry of the tick. Journal Protozoology, 27, 59-71[31]Xu Y, Zhang S, Huang X, Bayin C, Xuan X, Igarashi I, Fujisaki K, Kabeya H, Maruyama S, Mikami T. 2003. Seroepidemiologic studies on Babesia equi and Babesia caballi infections in horses in Jilin Province of China. Journal of Veterinary Medical Science, 65, 1015-1017[32]Yang P, Liu H E, Zhu Y D, Wang X W. 1964. The survey report on Theileriosis in dairy cattle. Journal of Gansu Agricultural University, 1, 38. (in Chinese) [33]Yin H, Liu G Y, Luo J X, Guan G Q, Li Y Q. 2004. Phylogenetic analysis of Theileria species transmitted by Haemaphysalis qinghariensis. Parasitology Research, 92, 36-42[34]Yokoyama N, Ueno A, Mizuno D, Kuboki N, Khukhuu A, Igarashi I, Miyahara T, Shiraishi T, Kudo R, Oshiro M. 2011. Genotypic diversity of Theileria orientalis detected from cattle grazing in Kumamoto and Okinawa prefectures of Japan. Journal of Veterinary Medical Science, 73, 305- 312. [35]Zhang S F, Xu Y T, Liang W F, Jiang T. 2003. The diagnosis of Theileria segenti by indirect fluorescent antibody. Chinese Journal of Veterinary Science and Technology, 33, 59-61[36](in Chinese) Zhao J L, Liu Z L. 1991. The research on the pathogen of buffalo babesiasis and the culture of Babesia bovis. Acta Veterinaria et Zootechnica Sinica, 22, 334-339. (in Chinese) |
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