Please wait a minute...
Journal of Integrative Agriculture  2026, Vol. 25 Issue (7): 3090-3093    DOI: 10.1016/j.jia.2026.03.022
Letter Advanced Online Publication | Current Issue | Archive | Adv Search |
From elite germplasm to transformation platform: Breaking recalcitrance in Tartary buckwheat

Zhen Wang1*, Tong Su1*, Kaixuan Zhang1, Yuqi He1, Zhirong Wang1, Alexander Betekhtin2, Meiliang Zhou1#

1 State Key Laboratory of Crop Gene Resources and Breeding/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

2 Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice 40-032, Poland

 Highlights 
An elite germplasm, G253, with superior morphogenic callus induction and proliferation capacity was identified.
A stable Agrobacterium-mediated transformation platform enabling transgenic plant generation was established.
An efficient protoplast transient transformation system was developed from the morphogenic callus.
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  

本研究选取了来自不同群系的20份苦荞种质,并系统评估了其未成熟合子胚的原胚细胞复合体(PECCs)诱导和增殖能力,从而筛选出了具有卓越的PECCs诱导和增殖能力的种质G253。基于G253的形态发生愈伤(MC),成功建立了一个稳定的农杆菌介导的遗传转化平台,获得的愈伤转化系中T-DNA整合的阳性率超过90%,并通过PECCs再生体系获得了转基因植株。此外,还基于苦荞MC开发了一个高效的原生质体瞬时转化系统。本研究为克服苦荞遗传转化的瓶颈、加速其分子育种进程提供了关键的种质资源和技术平台。



Received: 09 January 2026   Accepted: 06 February 2026 Online: 10 March 2026  
Fund: 

This study was supported by the National Natural Science Foundation of China (32241042, 32161143005, 32301794, 32401816, 32572314, 32501861, and W2512019), the National Key Research and Development Program of China (2023YFF1002500) and the National Science Centre of Poland, Research Project SONATA BIS 10 (Reg. 2020/38/E/NZ9/00033 awarded to AB).  

About author:  #Correspondence Meiliang Zhou, E-mail: zhoumeiliang@caas.cn * These authors contributed equally to this study.

Cite this article: 

Zhen Wang, Tong Su, Kaixuan Zhang, Yuqi He, Zhirong Wang, Alexander Betekhtin, Meiliang Zhou. 2026. From elite germplasm to transformation platform: Breaking recalcitrance in Tartary buckwheat. Journal of Integrative Agriculture, 25(7): 3090-3093.

Betekhtin A, Rojek M, Jaskowiak J, Milewska-Hendel A, Kwasniewska J, Kostyukova Y, Kurczynska E, Rumyantseva N, Hasterok R. 2017. Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn. PLoS ONE12, e0173537.

Chen Z, Debernardi J M, Dubcovsky J, Gallavotti A. 2022. Recent advances in crop transformation technologies. Nature Plants8, 1343–1351.

Dong Q, Zhao H, Zhao X, Lv B, Li Q, Wang X, Chen H, Li C, Wu Q. 2019. Callus induction of tartary buckwheat and enhancement of its flavonoids via FtCHS1 overexpression. PeerJ7, e27862v27861.

He Y, Zhang K, Li S, Lu X, Zhao H, Guan C, Huang X, Shi Y, Kang Z, Fan Y, Li W, Chen C, Li G, Long O, Chen Y, Hu M, Cheng J, Xu B, Chapman M A, Georgiev M I, et al. 2023. Multiomics analysis reveals the molecular mechanisms underlying virulence in Rhizoctonia and jasmonic acid-mediated resistance in Tartary buckwheat (Fagopyrum tataricum). Plant Cell35, 27732798.

He Y, Zhang K, Shi Y, Lin H, Huang X, Lu X, Wang Z, Li W, Feng X, Shi T, Chen Q, Wang J, Tang Y, Chapman M A, Germ M, Luthar Z, Kreft I, Janovska D, Meglic V, Woo S H, et al. 2024. Genomic insight into the origin, domestication, dispersal, diversification and human selection of Tartary buckwheat. Genome Biology25, 61.

Kostyukova Y A, Rumyantseva N I. 2018. The influence of explant cell differentiation on induction of embryogenic calli of Tartary Buckwheat. In: Book of Proceedings of the All-Russian Scientific Conference with International Participation and Schools of Young Scientists “Mechanisms of Resistance of Plants and Microorganisms to Unfavorable Environmental” (Parts I II). Siberian Institute of Plant Physiology and Biochemistry, Irkutsk. pp. 1280–1284.

Pinski A, Betekhtin A. 2023. Efficient Agrobacterium-mediated transformation and genome editing of Fagopyrum tataricumFrontiers in Plant Science8, 1270150.

Rumyantseva N I, Ensikat H J, Salnikov V V, Kostyukova Y A, Baluska F. 2003. Changes in the extracellular matrix surface network during cyclic reproduction of proembryonic cell complexes in the Fagopyrum tataricum (L.) Gaertn callus. Doklady Biological Sciences391, 375378.

Tomasiak A, Zhou M, Betekhtin A. 2022. Buckwheat in tissue culture research: Current status and future perspectives. International Journal of Molecular Sciences23, 2298.

Zaranek M, Perez-Perez R, Milewska-Hendel A, Betekhtin A, Grzebelus E. 2023. Promotive effect of phytosulfokine-peptide growth factor-on protoplast cultures development in Fagopyrum tataricum (L.) Gaertn. BMC Plant Biology23, 385.

Zhang K, He M, Fan Y, Zhao H, Gao B, Yang K, Li F, Tang Y, Gao Q, Lin T, Quinet M, Janovska D, Meglic V, Kwiatkowski J, Romanova O, Chrungoo N, Suzuki T, Luthar Z, Germ M, Woo S H, et al. 2021. Resequencing of global Tartary buckwheat accessions reveals multiple domestication events and key loci associated with agronomic traits. Genome Biology22, 23.

No related articles found!
No Suggested Reading articles found!