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Journal of Integrative Agriculture  2026, Vol. 25 Issue (7): 3094-3098    DOI: 10.1016/j.jia.2025.11.026
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Identification of a novel linezolid resistance gene, cfr(F), in Riemerella anatipestifer

Yihua Zhang1, 2, 3, 4, Zhishuang Yang1, 2, 3, 4, Mingshu Wang1, 2, 3, 4, 5, Renyong Jia1, 2, 3, 4, 5, Shun Chen1, 2, 3, 4, 5, Mafeng Liu1, 2, 3, 4, 5, Xinxin Zhao1, 2, 3, 4, 5, Qiao Yang1, 2, 3, 4, 5, Ying Wu1, 2, 3, 4, 5, Shaqiu Zhang1, 2, 3, 4, 5, Juan Huang1, 2, 3, 4, 5, Xumin Ou1, 2, 3, 4, 5, Di Sun1, 2, 3, 4, 5, Bin Tian1, 2, 3, 4, 5, Yu He1, 2, 3, 4, 5, Zhen Wu1, 2, 3, 4, 5, Anchun Cheng1, 4, 5, 6#, Dekang Zhu1, 2, 3, 4, 5#

1 Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

2 Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu 611130, China

3 Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Chengdu 611130, China

4 International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China

5 Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education, Chengdu 611130, China

6 Institute of Veterinary Immunology and Green Drugs/Veterinary Department, College of Animal Science/State Key Laboratory of Green Pesticide, Guizhou University, Guiyang 550025, China

 Highlights 
A novel linezolid resistance gene, cfr(F), was identified in Riemerella anatipestifer.
cfr(F) confers cross-resistance to PhLOPSA antibiotics, including linezolid.
Riemerella anatipestifer is a potential reservoir for linezolid resistance gene dissemination.
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摘要  

抗生素耐药性仍是人类与兽医学领域面临的最严峻挑战之一。鸭疫里默氏菌一种重要的禽类病原,已被证实具有多重耐药性。本研究通过全基因组测序,从鸭疫里默氏菌临床菌株RCAD1242中鉴定并表征了一个新型利奈唑胺耐药基因cfr(F)。通过将cfr(F)克隆至敏感株鸭疫里默氏菌ATCC 11845与多杀性巴氏杆菌DY120818进行药敏试验证实该基因可介导对酰胺醇类、林可酰胺类、恶唑烷酮类、截短侧耳素及A型链阳菌素的交叉耐药,即PhLOPSA表型其氨基酸序列与已报道的cfr基因的相似性仅为32.9%-43.8%。重组菌株对氟苯尼考、利奈唑胺等抗生素的最小抑菌浓度MIC提升2-1024倍。目前cfr(F)基因已在中国、韩国和美国被检出,呈现跨地域传播的趋势。鉴于鸭疫里默氏菌在禽广泛流行并具备可移动遗传元件交换能力,该菌可能成为恶唑烷酮类耐药性向临床重要人类病原传播的潜在储存库,构成公共卫生威胁。本研究强调加强“同一健康(One Health”框架下的耐药性监测体系,并在动物环境人类领域推行科学、审慎的抗菌药物使用策略,以遏制耐药基因的跨物种传播。



Received: 07 June 2025   Accepted: 23 October 2025 Online: 14 November 2025  
Fund: This work was supported by the National Key Research and Development Program of China (2023YFD1800200), the Key Program of the National Natural Science Foundation of China (U24A20451), the Earmarked Fund for China Agriculture Research System (CARS-42-17), and the Sichuan Veterinary Medicine and the Drug Innovation Group of China Agricultural Research System (SCCXTD-2025-18).
About author:  Yihua Zhang, E-mail: zyh18881577639@163.com; #Correspondence Dekang Zhu, E-mail: zdk24@sicau.edu.cn; Anchun Cheng, E-mail: chenganchun@vip.163.com

Cite this article: 

Yihua Zhang, Zhishuang Yang, Mingshu Wang, Renyong Jia, Shun Chen, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Di Sun, Bin Tian, Yu He, Zhen Wu, Anchun Cheng, Dekang Zhu. 2026. Identification of a novel linezolid resistance gene, cfr(F), in Riemerella anatipestifer. Journal of Integrative Agriculture, 25(7): 3094-3098.

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