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Journal of Integrative Agriculture  2026, Vol. 25 Issue (8): 3508-3510    DOI: 10.1016/j.jia.2025.12.042
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Coronavirus-like particles package the negative-strand genome of coronavirus

Xinyu Yang*, Wenzhen Qin*, Ning Kong*, Yuchang Liu, Guangzhi Tong, Tongling Shan#

Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China

 Highlights 
The particles of coronavirus package the negative-sense genome of coronavirus into the coronavirus-like particles.
In PEDV particles purified by sucrose density gradient centrifugation and treated with antibodies and nuclease, approximately 20 to 46% contain negative-sense genomes.
Coronavirus-like particles are capable of adsorption and internalization.
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摘要  

冠状病毒是单链正义 RNA 病毒。在 PEDV 吸附和内化后及病毒负链 RNA 合成前,我们同时检测到了冠状病毒的正链和负链序列。为解释上述发现,本研究证实α、β和δ冠状病毒属的冠状病毒病毒粒子中包裹病毒的负链基因组。通过蔗糖密度梯度离心纯化,并经抗体和核酸酶处理的 PEDV 颗粒中,约 20% 至 46% 含有负链基因组,且这些病毒颗粒具备吸附和内化的能力。该研究解释了冠状病毒在吸附和内化后检测到病毒的负链RNA的发现,也表明了冠状病毒在组装过程中不仅包装病毒正链基因组,也会同时包装了病毒负链基因组。



Received: 28 September 2025   Accepted: 25 November 2025 Online: 22 December 2025  
Fund: 

This work was supported by the National Key Research and Development Programs of China (2023YFD1801300) and the National Natural Science Foundation of China (32272999).

About author:  #Correspondence Tongling Shan, E-mail: shantongling@shvri.ac.cn * These authors contributed equally to this study.

Cite this article: 

Xinyu Yang, Wenzhen Qin, Ning Kong, Yuchang Liu, Guangzhi Tong, Tongling Shan. 2026. Coronavirus-like particles package the negative-strand genome of coronavirus. Journal of Integrative Agriculture, 25(8): 3508-3510.

Maier H, Bickerton E. 2020. Coronaviruses Methods and ProtocolsMethods and Protocols. Humana Press, New York, NY.

Malone B, Urakova N, Snijder E J, Campbell E A. 2022. Structures and functions of coronavirus replication-transcription complexes and their relevance for SARS-CoV-2 drug design. Nature Reviews Molecular Cell Biology23, 21–39.

Shan T, Yang S, Wang H, Wang H, Zhang J, Gong G, Xiao Y, Yang J, Wang X, Lu J, Zhao M, Yang Z, Lu X, Dai Z, He Y, Chen X, Zhou R, Yao Y, Kong N, Zeng J, et al. 2022. Virome in the cloaca of wild and breeding birds revealed a diversity of significant viruses. Microbiome10, 60.

Sola I, Almazán F, Zúñiga S, Enjuanes L. 2015. Continuous and discontinuous RNA synthesis in coronaviruses. Annual Review of Virology, 2, 265–288.

Thi Nhu Thao T, Labroussaa F, Ebert N, V’Kovski P, Stalder H, Portmann J, Kelly J, Steiner S, Holwerda M, Kratzel A, Gultom M, Schmied K, Laloli L, Hüsser L, Wider M, Pfaender S, Hirt D, Cippà V, Crespo-Pomar S, Schröder S, et al. 2020. Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform. Nature582, 561–565.

Walker P J, Siddell S G, Lefkowitz E J, Mushegian A R, Adriaenssens E M, Alfenas-Zerbini P, Dempsey D M, Dutilh B E, García M L, Curtis Hendrickson R, Junglen S, Krupovic M, Kuhn J H, Lambert A J, Łobocka M, Oksanen H M, Orton R J, Robertson D L, Rubino L, Sabanadzovic S, et al. 2022. Recent changes to virus taxonomy ratified by the International Committee on Taxonomy of Viruses. Archives of Virology167, 2429–2440.

Woo P C Y, de Groot R J, Haagmans B, Lau S K P, Neuman B W, Perlman S, Sola I, van der Hoek L, Wong A C P, Yeh S H. 2023. ICTV virus taxonomy profile: Coronaviridae. Journal of General Virology104, 001843.

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